Construct: ORF ccsbBroad304_13922
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010879.1_s304c1, BRDN0000398667
- Derived from:
- ccsbBroadEn_13922
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR2K (5440)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13922
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5440 | POLR2K | RNA polymerase II subunit K | NM_005034.4 | 99.4% | 98.2% | 173G>C |
| 2 | mouse | 17749 | Polr2k | polymerase (RNA) II (DNA di... | NM_023127.3 | 91.9% | 96.5% | (many diffs) |
| 3 | mouse | 102641086 | LOC102641086 | DNA-directed RNA polymerase... | XM_006536721.2 | 91.3% | 94.8% | (many diffs) |
| 4 | mouse | 100862456 | LOC100862456 | DNA-directed RNA polymerase... | XM_006537451.2 | 91.3% | 94.8% | (many diffs) |
| 5 | mouse | 17749 | Polr2k | polymerase (RNA) II (DNA di... | NM_001039368.2 | 53.8% | 56.5% | (many diffs) |
| 6 | mouse | 108167692 | Gm46136 | predicted gene, 46136 | XR_001779328.1 | 1.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 240
- ORF length:
- 174
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cacccagaag gacgttcaac ctccaaagca gcaaccaatg atatatatct 121 gtggagagtg tcacacagaa aatgaaataa aatctaggga tccaatcaga tgcagagaat 181 gtggatacag aataatgtac aagaaaagga ctaaaagatt ggtcgttttt gatgctccat 241 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 301 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 361 ggatta