Construct: ORF ccsbBroad304_14067

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000733.1_s304c1, BRDN0000401985
Derived from:
ccsbBroadEn_14067
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
XRN2 (22803)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14067
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22803 XRN2 5'-3' exoribonuclease 2 NM_012255.5 99.9% 77.3% 2197_2198insA
2 human 22803 XRN2 5'-3' exoribonuclease 2 NM_001317960.1 91.6% 69.2% (many diffs)
3 human 22803 XRN2 5'-3' exoribonuclease 2 XM_017027723.2 55.3% 32.8% 0_1ins1272;925_926insA
4 human 22803 XRN2 5'-3' exoribonuclease 2 XM_017027722.1 49.4% 58.5% (many diffs)
5 human 22803 XRN2 5'-3' exoribonuclease 2 XM_017027724.1 39.3% 46.6% (many diffs)
6 mouse 24128 Xrn2 5'-3' exoribonuclease 2 NM_011917.2 92.2% 75.6% (many diffs)
7 mouse 24128 Xrn2 5'-3' exoribonuclease 2 XM_011239519.2 76% 92.4% (many diffs)
8 mouse 24128 Xrn2 5'-3' exoribonuclease 2 XM_006499450.3 75.4% 58.3% (many diffs)
9 mouse 24128 Xrn2 5'-3' exoribonuclease 2 XM_006499451.2 49.7% 31.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2328
ORF length:
2259
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggagtcccg gcgttcttcc gctggctcag ccgcaagtac ccgtccatca
121tagtcaactg cgtggaagag aagccaaaag aatgcaatgg tgtaaagatt ccagttgatg
181ccagtaaacc taatccaaat gatgtggagt ttgataatct gtatttggat atgaatggaa
241tcatccatcc ctgtactcat cctgaagaca aaccagcacc aaaaaatgaa gatgaaatga
301tggttgcaat ttttgagtac attgacagac ttttcagtat tgtaagacca agaagacttc
361tctacatggc aatagatgga gtggcaccac gtgctaaaat gaaccagcag cgttcaagga
421ggttcagggc atcaaaagaa ggaatggaag cagcagtcga gaagcagcga gtcagggaag
481aaatattggc aaaaggtggc tttcttcctc cagaagaaat aaaagaaaga tttgacagca
541actgtattac accaggaact gaattcatgg acaatcttgc taaatgcctt cgctattaca
601tagctgatcg tttaaataat gaccctgggt ggaaaaattt gacagttatt ttatctgatg
661ctagtgctcc tggtgaagga gaacataaaa tcatggatta cattagaagg caaagagccc
721agcctaacca tgacccaaat actcatcatt gtttatgtgg agcagatgct gatctcatta
781tgcttggcct tgccacacat gaaccgaact ttaccattat tagagaagaa ttcaaaccaa
841acaagcccaa accatgtggt ctttgtaatc agtttggaca tgaggtcaaa gattgtgaag
901gtttgccaag agaaaagaag ggaaagcatg atgaacttgc cgatagtctt ccttgtgcag
961aaggagagtt tatcttcctt cggcttaatg ttcttcgtga gtatttggaa agagaactca
1021caatggccag cctaccattc acatttgatg ttgagaggag cattgatgac tgggttttca
1081tgtgcttctt tgtgggaaat gacttcctcc ctcatttgcc atcgttagag attagggaaa
1141atgcaattga ccgtttggtt aacatataca aaaatgtggt acacaaaact gggggttacc
1201ttacagaaag tggttatgtc aatctgcaaa gagtacagat gatcatgtta gcagttggtg
1261aagttgagga tagcattttt aaaaagagaa aggatgatga ggacagtttt agaagacgac
1321agaaagaaaa aagaaagaga atgaagagag atcaaccagc tttcactcct agtggaatat
1381taactcctca tgccttgggt tcaagaaatt caccaggttc tcaagtagcc agtaatccga
1441gacaagcagc ctatgaaatg aggatgcaga ataactctag tccttcgata tctcctaata
1501cgagtttcac atctgatggc tccccgtctc cattaggagg aattaagcga aaagcagaag
1561acagtgacag tgaacctgag ccagaggata atgtcaggtt atgggaagct ggctggaagc
1621agcggtacta caagaacaaa tttgatgtgg atgcagctga tgagaaattc cgtcggaaag
1681ttgtgcagtc gtacgttgaa ggactttgct gggttcttag atattattac cagggctgtg
1741cttcctggaa gtggtattat ccatttcatt atgcaccatt tgcttcagac tttgaaggca
1801ttgcagacat gccatctgat tttgagaagg gtacgaaacc gtttaaacca ctagaacaac
1861ttatgggggt atttccagct gcaagtggta attttctacc tccatcatgg cggaagctca
1921tgagtgatcc tgattctagt ataattgact tctatcctga agattttgct attgatttga
1981atgggaagaa atatgcatgg caaggtgttg ctctcttgcc attcgtggat gagcgaaggc
2041tacgagctgc cctagaagag gtatacccag acctcactcc agaagagacc agaagaaaca
2101gccttggagg tgatgtctta tttgtgggga aacatcaccc actccatgac ttcattttag
2161agctgtacca gacaggttcc acagagccag tggaggtacc ccctgaacta tgtcatggga
2221ttcaaggaaa gttttctttg gatgaagaag ccattcttcc agatcaaaat agtatgttct
2281cctgttccta tgttaaggga tctgacacag aacactgtag tcagtattaa ttttaaagac
2341ccacagtttg ctgaagatta catttttaaa gctgtaatgc ttccaggagc aagaaagcca
2401gcagcagtac tgaaacctag tgactgggaa aaatccagca atggacggca gtggaagcct
2461cagcttggct ttaaccgtga ccggaggcct gtgcacctgg atcaggcagc cttcaggact
2521ttgggccatg tgatgccaag aggctcagga actggcattt acagcaatgc tgcaccacca
2581cctgtgactt accagggaaa cttatacagg ccgcttttga gaggacaagc ccagattcca
2641aaacttatgt caaatatgag gccccaggat tcctggcgag gtcctcctcc ccttttccag
2701cagcaaaggt ttgacagagg cgttggggct gaacctctgc tcccatggaa ccggatgctg
2761caaacccaga atgcagcctt ccagccaaac cagtaccaga tgctagctgg gcctggtggg
2821tatccaccca gacgagatga tcgtggaggg agacagggat atcccagaga aggaaggaaa
2881taccctttgc caccaccctc aggaagatac aattggaatt tgccaacttt cttgtacaaa
2941gtggttggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct
3001agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggatta

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