Construct: ORF ccsbBroad304_14237
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002714.1_s304c1, BRDN0000400250
- Derived from:
- ccsbBroadEn_14237
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTBP2 (58155)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14237
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | NM_001300989.2 | 99.9% | 100% | 1584C>A |
| 2 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | NM_021190.3 | 99.7% | 99.8% | 1466_1467insAAG;1581C>A |
| 3 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | NM_001300985.2 | 99% | 99% | 903_917del;1599C>A |
| 4 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | NM_001300988.1 | 98.8% | 98.8% | 903_917del;1481_1482insAAG;1596C>A |
| 5 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | NM_001300986.2 | 98.4% | 98.5% | 8_31del;1608C>A |
| 6 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XM_011541875.2 | 98.2% | 98.3% | 8_31del;1490_1491insAAG;1605C>A |
| 7 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XM_011541876.2 | 97.5% | 97.6% | 8_31del;927_941del;1623C>A |
| 8 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XM_011541874.2 | 97.3% | 97.4% | (many diffs) |
| 9 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | NM_001300987.2 | 95.8% | 95.2% | (many diffs) |
| 10 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XM_017001951.1 | 95.8% | 95.2% | (many diffs) |
| 11 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | NM_001300990.2 | 90.1% | 90.2% | 0_1ins156;1428C>A |
| 12 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XR_001737347.2 | 49.9% | (many diffs) | |
| 13 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XR_001737346.2 | 49.7% | (many diffs) | |
| 14 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XR_946723.3 | 49.2% | (many diffs) | |
| 15 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XR_946722.2 | 49.1% | (many diffs) | |
| 16 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XR_946721.2 | 49% | (many diffs) | |
| 17 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | XR_946720.2 | 48.9% | (many diffs) | |
| 18 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | NR_125356.1 | 45.6% | (many diffs) | |
| 19 | human | 58155 | PTBP2 | polypyrimidine tract bindin... | NR_125357.2 | 12.9% | (many diffs) | |
| 20 | mouse | 56195 | Ptbp2 | polypyrimidine tract bindin... | NM_019550.2 | 93.6% | 99.6% | (many diffs) |
| 21 | mouse | 56195 | Ptbp2 | polypyrimidine tract bindin... | NM_001310711.1 | 93.4% | 99.4% | (many diffs) |
| 22 | mouse | 56195 | Ptbp2 | polypyrimidine tract bindin... | XM_006501731.1 | 92.9% | 98.6% | (many diffs) |
| 23 | mouse | 56195 | Ptbp2 | polypyrimidine tract bindin... | XM_006501732.1 | 92.7% | 98.5% | (many diffs) |
| 24 | mouse | 56195 | Ptbp2 | polypyrimidine tract bindin... | XR_001783719.1 | 41.3% | (many diffs) | |
| 25 | mouse | 56195 | Ptbp2 | polypyrimidine tract bindin... | XR_375550.2 | 40.4% | (many diffs) | |
| 26 | mouse | 56195 | Ptbp2 | polypyrimidine tract bindin... | XR_001783718.1 | 38.5% | (many diffs) | |
| 27 | mouse | 56195 | Ptbp2 | polypyrimidine tract bindin... | XR_375549.1 | 38.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cggaatcgtc actgaggttg cagttggcgt gaagagagga tctgacgaac 121 tactctcagg cagtgttctc agtagtccga actctaatat gagcagcatg gtagttacag 181 ccaatggtaa tgatagtaaa aaatttaaag gagaagataa aatggatggt gctccttctc 241 gtgtacttca tattcgaaaa ttacctgggg aagtaacaga aactgaagtt attgctttag 301 gcttaccttt tggtaaggtg accaacatcc ttatgctgaa aggaaaaaat caggcatttt 361 tggaactagc aaccgaggaa gcagctatta ctatggttaa ttactattct gctgtgacac 421 ctcatcttcg taaccaacca atatatatcc agtactcgaa tcacaaagaa ctaaagacag 481 ataatacatt aaaccaacgt gctcaggcag ttcttcaagc tgtgacagct gtccagacag 541 caaatactcc tcttagtggc accacagtta gcgagagtgc agtgactcca gcccagagtc 601 cagtacttag aataattatt gacaacatgt actaccctgt aacacttgat gttcttcacc 661 aaatattttc taagtttggt gctgtattga agataatcac atttacaaaa aataaccagt 721 ttcaagcttt gctccagtat ggtgatccag taaatgctca acaagcaaaa ctagccctag 781 atggtcagaa tatttataat gcctgctgta ccctaaggat tgatttttcc aaacttgtga 841 atttgaatgt aaaatacaac aatgataaaa gtagggatta tactcgacct gatcttccat 901 ctggggatgg acaacctgca ttggacccag ctattgctgc agcatttgcc aaggagacat 961 ccctcttagc tgttccagga gctctgagtc ctttggccat tccaaatgct gctgcagcag 1021 ctgctgcagc tgctgctggc cgagtgggta tgcctggagt ctcagctggt ggcaatacag 1081 tcctgttggt tagcaattta aatgaagaga tggttacgcc ccaaagtctg tttaccctct 1141 tcggtgttta tggagatgtg cagcgtgtga agattttata caataagaaa gacagcgctc 1201 taatacagat ggctgatgga aaccaatcac aacttgccat gaatcatctt aatggacaga 1261 aaatgtatgg aaaaattatt cgtgttactc tgtctaaaca tcagactgta cagctacctc 1321 gagagggact tgatgatcaa gggctaacaa aagattttgg taattcccca ttgcatcgtt 1381 ttaagaaacc tggatccaaa aattttcaaa acatttttcc tccttctgcc acccttcacc 1441 tatctaatat ccctccatca gtagcagaag aggatctacg aacactgttc gctaacactg 1501 ggggcactgt gaaagcattt aagttttttc aaagagatca caaaatggct cttcttcaga 1561 tggcaacagt ggaagaagct attcaggcct tgattgatct tcataattat aaccttggag 1621 aaaaccatca tctgagagtg tctttctcaa agtcaacaat ttaccaactt tcttgtacaa 1681 agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgagc 1741 tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta