Transcript: Human XR_946722.2

PREDICTED: Homo sapiens polypyrimidine tract binding protein 2 (PTBP2), transcript variant X7, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PTBP2 (58155)
Length:
3131
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_946722.2
NBCI Gene record:
PTBP2 (58155)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_946722.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000001111 CCCTAGATGGTCAGAATATTT pLKO.1 803 3UTR 100% 15.000 21.000 N PTBP2 n/a
2 TRCN0000418376 GCAGCTATTACTATGGTTAAT pLKO_005 409 3UTR 100% 13.200 18.480 N PTBP2 n/a
3 TRCN0000001110 GCCACCCTTCACCTATCTAAT pLKO.1 1422 3UTR 100% 13.200 18.480 N PTBP2 n/a
4 TRCN0000435789 GTTCAATGTCATCACCTATTT pLKO_005 1691 3UTR 100% 13.200 18.480 N PTBP2 n/a
5 TRCN0000422572 TAAGAAAGACAGCGCTCTAAT pLKO_005 1178 3UTR 100% 13.200 18.480 N PTBP2 n/a
6 TRCN0000109231 GCTGTACCCTAAGGATTGATT pLKO.1 833 3UTR 100% 5.625 7.875 N Ptbp2 n/a
7 TRCN0000317485 GCTGTACCCTAAGGATTGATT pLKO_005 833 3UTR 100% 5.625 7.875 N Ptbp2 n/a
8 TRCN0000419376 CCTGTTGGTTAGCAATTTAAA pLKO_005 1110 3UTR 100% 15.000 10.500 N PTBP2 n/a
9 TRCN0000436348 CCAGTATGGTGATCCAGTAAA pLKO_005 762 3UTR 100% 13.200 9.240 N PTBP2 n/a
10 TRCN0000422912 AGCCCAGAGTCCAGTACTTAG pLKO_005 618 3UTR 100% 10.800 7.560 N PTBP2 n/a
11 TRCN0000001112 CATCTTCGTAACCAACCAATA pLKO.1 451 3UTR 100% 10.800 7.560 N PTBP2 n/a
12 TRCN0000001109 CTGTTATCATTCCTTGGTTAT pLKO.1 1789 3UTR 100% 10.800 7.560 N PTBP2 n/a
13 TRCN0000001113 CTCCTTCTCGTGTACTTCATA pLKO.1 260 3UTR 100% 5.625 3.938 N PTBP2 n/a
14 TRCN0000109230 GCTGTTATCATTCCTTGGTTA pLKO.1 1788 3UTR 100% 4.950 3.465 N Ptbp2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_946722.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14237 pDONR223 100% 49.1% None (many diffs) n/a
2 ccsbBroad304_14237 pLX_304 0% 49.1% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000473640 AAGAATCTGTTACTACTTCAAATT pLX_317 32.9% 49.1% V5 (not translated due to frame shift) (many diffs) n/a
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