Construct: ORF ccsbBroad304_14312

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF007769.1_s304c1, BRDN0000402024
• Derived from: ccsbBroadEn_14312
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: CAPS2 (84698)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_14312
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	84698	CAPS2	calcyphosine 2	XM_017020039.1	58.1%	58%	1_438del;454G>A
2	human	84698	CAPS2	calcyphosine 2	NM_001286548.3	53.3%	53.1%	1_534del;550G>A
3	human	84698	CAPS2	calcyphosine 2	NM_001355030.2	53.3%	53.1%	1_534del;550G>A
4	human	84698	CAPS2	calcyphosine 2	NM_001355031.2	53.3%	53.1%	1_534del;550G>A
5	human	84698	CAPS2	calcyphosine 2	NM_001355032.2	53.3%	53.1%	1_534del;550G>A
6	human	84698	CAPS2	calcyphosine 2	NM_001355033.2	53.3%	53.1%	1_534del;550G>A
7	human	84698	CAPS2	calcyphosine 2	XM_017020036.1	53.3%	53.1%	1_534del;550G>A
8	human	84698	CAPS2	calcyphosine 2	NM_001355026.2	42.6%	42.5%	1_819del;835G>A
9	human	84698	CAPS2	calcyphosine 2	XM_005269194.3	41.8%	41.7%	1_438del;454G>A;879_879delTins172
10	human	84698	CAPS2	calcyphosine 2	XM_005269192.1	40%	39.9%	1_912del;928G>A
11	human	84698	CAPS2	calcyphosine 2	XM_011538888.1	40%	39.8%	1_915del;931G>A
12	human	84698	CAPS2	calcyphosine 2	NM_001355025.2	38.5%	38.4%	1_972del;988G>A
13	human	84698	CAPS2	calcyphosine 2	XM_011538887.2	37.4%	37.3%	1_1020del;1036G>A
14	human	84698	CAPS2	calcyphosine 2	NM_001355024.2	36.1%	35.9%	1_1080del;1096G>A
15	human	84698	CAPS2	calcyphosine 2	XM_024449226.1	35.9%	35.8%	1_1086del;1102G>A
16	human	84698	CAPS2	calcyphosine 2	NM_001355023.2	35.7%	35.6%	1_1098del;1114G>A
17	human	84698	CAPS2	calcyphosine 2	XM_011538886.2	35.3%	35.2%	1_1116del;1132G>A
18	human	84698	CAPS2	calcyphosine 2	XM_006719649.2	34.9%	34.8%	1_1134del;1150G>A
19	human	84698	CAPS2	calcyphosine 2	XM_011538878.1	33.8%	33.7%	1_1194del;1210G>A
20	human	84698	CAPS2	calcyphosine 2	XM_011538889.3	33.8%	33.7%	1_1194del;1210G>A
21	human	84698	CAPS2	calcyphosine 2	XM_006719648.2	33.1%	33%	1_1230del;1246G>A
22	human	84698	CAPS2	calcyphosine 2	NM_001355027.2	27.7%	27.6%	1_972del;988G>A;1413_1413delTins172
23	human	84698	CAPS2	calcyphosine 2	NM_001286547.3	27.5%	27.4%	1_984del;1000G>A;1425_1425delTins172
24	human	84698	CAPS2	calcyphosine 2	NM_032606.5	23.8%	23.7%	1_1230del;1246G>A;1671_1671delTins172
25	human	84698	CAPS2	calcyphosine 2	XR_944789.1	17%		1_1427del;1443G>A;2040_3585del
26	human	84698	CAPS2	calcyphosine 2	NR_149156.2	14.5%		1_681del;697G>A;1294_4207del
27	human	84698	CAPS2	calcyphosine 2	NR_149155.2	14.4%		1_709del;725G>A;1322_4235del
28	human	84698	CAPS2	calcyphosine 2	NR_149161.2	14.2%		1_755del;771G>A;1368_4281del
29	human	84698	CAPS2	calcyphosine 2	NR_149159.2	13.2%		1_1085del;1101G>A;1698_4611del
30	human	84698	CAPS2	calcyphosine 2	NR_149158.2	13.2%		(many diffs)
31	human	84698	CAPS2	calcyphosine 2	NR_149157.2	13.1%		1_1113del;1129G>A;1726_4639del
32	human	84698	CAPS2	calcyphosine 2	NR_149160.2	13%		1_1145del;1161G>A;1758_4671del

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

69

ORF end:

681

ORF length:

612

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcaccat ggaacaggag gataatataa tcattcaaga aaccaatgat aggctggtct
121	tcaaagcaat tcaagatgtg ctaaaagaaa aactacataa aagaggtgtt cgtattttga
181	ctggattggg aaaatatttt caacagttgg acaaggaagg aaatggactt ttagataagg
241	cagattttaa gcaagctcta aaagtgtttc acttagaagt gtctgaaaag gattttgagt
301	ctgcatggct aattctgaat gacaatggca atggcaaggt tgattatgga gaattcaaac
361	gtggtattat tggtgaaatg aatgaataca ggaaatcata tgttcgaaag gcctttatga
421	aactggattt caacaaaagt ggcagtgtgc ctattataaa cataagaaaa tgttactgtg
481	caaagaagca ttctcaagta atttcaggcc attcaacaga ggaagaaatc aaatcatcct
541	ttctagaaac attaaaagtt gcctgcagca agtctgatga agtgtcatat ggtgaatttg
601	aagattacta tgaaggttta agtataggaa tagtagatga tgaagacttt gttaacatct
661	tacgtactcc atgggggatt ttgccaactt tcttgtacaa agtggttggt aagcctatcc
721	ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
781	ttaagtcgac aatcaacctc tggatta

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