Construct: ORF ccsbBroad304_14517

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004738.1_s304c1, BRDN0000403214
Derived from:
ccsbBroadEn_14517
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
GPR89A (653519)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14517
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 653519 GPR89A G protein-coupled receptor 89A NM_001097612.2 99.8% .8% 12C>T;14delT
2 human 51463 GPR89B G protein-coupled receptor 89B NM_001350180.1 99.8% .8% 12G>T;14delT
3 human 51463 GPR89B G protein-coupled receptor 89B NM_016334.5 99.8% .8% 12G>T;14delT
4 human 653519 GPR89A G protein-coupled receptor 89A XM_006711492.4 99.8% .8% 12C>T;14delT
5 human 653519 GPR89A G protein-coupled receptor 89A NM_001097613.2 94.5% .9% 0_1ins74
6 human 51463 GPR89B G protein-coupled receptor 89B NM_001350181.1 94.5% .9% 0_1ins74
7 human 51463 GPR89B G protein-coupled receptor 89B NM_001350182.1 94.5% .9% 0_1ins74
8 human 51463 GPR89B G protein-coupled receptor 89B XM_005277402.4 94.5% .9% 0_1ins74
9 human 653519 GPR89A G protein-coupled receptor 89A XM_011509908.2 94.5% .9% 0_1ins74
10 human 653519 GPR89A G protein-coupled receptor 89A XM_011509909.2 94.5% .9% 0_1ins74
11 human 51463 GPR89B G protein-coupled receptor 89B XM_017001448.2 94.5% .9% 0_1ins74
12 human 51463 GPR89B G protein-coupled receptor 89B NM_001350183.1 73.6% 1.1% 0_1ins359
13 human 51463 GPR89B G protein-coupled receptor 89B NM_001350184.1 73.6% 1.1% 0_1ins359
14 human 653519 GPR89A G protein-coupled receptor 89A XM_006711493.3 73.6% 1.1% 0_1ins359
15 human 51463 GPR89B G protein-coupled receptor 89B XM_011509612.2 73.6% 1.1% 0_1ins359
16 human 653519 GPR89A G protein-coupled receptor 89A NR_036541.1 68% (many diffs)
17 human 51463 GPR89B G protein-coupled receptor 89B XR_001737221.1 62.2% (many diffs)
18 human 51463 GPR89B G protein-coupled receptor 89B XM_017001449.1 55% 1.5% (many diffs)
19 human 653519 GPR89A G protein-coupled receptor 89A XM_017002150.2 55% 1.5% (many diffs)
20 human 653519 GPR89A G protein-coupled receptor 89A XR_001737373.2 54.1% (many diffs)
21 human 51463 GPR89B G protein-coupled receptor 89B XM_011509613.2 53.6% 1.6% (many diffs)
22 human 653519 GPR89A G protein-coupled receptor 89A XM_011509910.3 53.6% 1.6% (many diffs)
23 mouse 67549 Gpr89 G protein-coupled receptor 89 NM_026229.4 88.3% .8% (many diffs)
24 mouse 67549 Gpr89 G protein-coupled receptor 89 XM_017319696.1 65.1% .8% (many diffs)
25 mouse 67549 Gpr89 G protein-coupled receptor 89 XR_375569.3 25.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
93
ORF length:
27
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag tttccttacg actccagcat catgattacc tcccagatac tattttttgg
121atttgggtgg cttttcttca tgcgccaatt gtttaaagac tatgagatac gtcagtatgt
181tgtacaggtg atcttctccg tgacgtttgc attttcttgc accatgtttg agctcatcat
241ctttgaaatc ttaggagtat tgaatagcag ctcccgttat tttcactgga aaatgaacct
301gtgtgtaatt ctgctgatcc tggttttcat ggtgcctttt tacattggct attttattgt
361gagcaatatc cgactactgc ataaacaacg actgcttttt tcctgtctct tatggctgac
421ctttatgtat ttcttctgga aactaggaga tccctttccc attctcagcc caaaacatgg
481gatcttatcc atagaacagc tcatcagccg ggttggtgtg attggagtga ctctcatggc
541tcttctttct ggatttggtg ctgtcaactg cccatacact tacatgtctt acttcctcag
601gaatgtgact gacacggata ttctagccct ggaacggcga ctgctgcaaa ccatggatat
661gatcataagc aaaaagaaaa ggatggcaat ggcacggaga acaatgttcc agaaggggga
721agtgcataac aaaccatcag gtttctgggg aatgataaaa agtgttacca cttcagcatc
781aggaagtgaa aatcttactc ttattcaaca ggaagtggat gctttggaag aattaagcag
841gcagcttttt ctggaaacag ctgatctata tgctaccaag gagagaatag aatactccaa
901aaccttcaag gggaaatatt ttaattttct tggttacttt ttctctattt actgtgtttg
961gaaaattttc atggctacca tcaatattgt ttttgatcga gttgggaaaa cggatcctgt
1021cacaagaggc attgagatca ctgtgaatta tctgggaatc caatttgatg tgaagttttg
1081gtcccaacac atttccttca ttcttgttgg aataatcatc gtcacatcca tcagaggatt
1141gctgatcact cttaccaagt tcttttatgc catctctagc agtaagtcct ccaatgtcat
1201tgtcctgcta ttagcacaga taatgggcat gtactttgtc tcctctgtgc tgctgatccg
1261aatgagtatg cctttagaat accgcaccat aatcactgaa gtccttggag aactgcagtt
1321caacttctat caccgttggt ttgatgtgat cttcctggtc agcgctctct ctagcatact
1381cttcctctat ttggctcaca aacaggcacc agagaagcaa atggcacctt gcccaacttt
1441cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg attctacgta
1501gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggatta

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