Construct: ORF ccsbBroad304_14553

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018930.1_s304c1, BRDN0000459414
Derived from:
ccsbBroadEn_14553
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
BRAF (673)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14553
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 673 BRAF B-Raf proto-oncogene, serin... NM_004333.6 93.1% 26.3% (many diffs)
2 human 673 BRAF B-Raf proto-oncogene, serin... NM_001354609.2 92.7% 26.3% (many diffs)
3 human 673 BRAF B-Raf proto-oncogene, serin... NM_001374244.1 88.5% 25% (many diffs)
4 human 673 BRAF B-Raf proto-oncogene, serin... NM_001374258.1 88.1% 25% (many diffs)
5 human 673 BRAF B-Raf proto-oncogene, serin... XM_017012559.1 82.9% 25.3% (many diffs)
6 human 673 BRAF B-Raf proto-oncogene, serin... XR_001744857.1 44.8% (many diffs)
7 human 673 BRAF B-Raf proto-oncogene, serin... XR_001744858.1 20.5% (many diffs)
8 mouse 109880 Braf Braf transforming gene XM_006505359.3 77% 22.4% (many diffs)
9 mouse 109880 Braf Braf transforming gene XM_006505358.3 76.7% 22.4% (many diffs)
10 mouse 109880 Braf Braf transforming gene XM_011241139.2 76.4% 22.6% (many diffs)
11 mouse 109880 Braf Braf transforming gene XM_011241138.2 75.4% 22.2% (many diffs)
12 mouse 109880 Braf Braf transforming gene NM_139294.5 75.4% 19.3% (many diffs)
13 mouse 109880 Braf Braf transforming gene XM_011241136.2 75.1% 19.4% (many diffs)
14 mouse 109880 Braf Braf transforming gene XM_011241137.1 74.5% 18.8% (many diffs)
15 mouse 109880 Braf Braf transforming gene XM_006505355.3 73.3% 21.4% (many diffs)
16 mouse 109880 Braf Braf transforming gene XM_011241135.2 73.1% 21.6% (many diffs)
17 mouse 109880 Braf Braf transforming gene XM_006505353.3 72.4% 21.1% (many diffs)
18 mouse 109880 Braf Braf transforming gene XM_011241134.2 72.1% 21.3% (many diffs)
19 mouse 109880 Braf Braf transforming gene XM_011241140.2 51.7% 27.2% (many diffs)
20 mouse 109880 Braf Braf transforming gene XR_001785085.1 38.3% (many diffs)
21 mouse 109880 Braf Braf transforming gene XR_001785086.1 34.9% (many diffs)
22 mouse 109880 Braf Braf transforming gene XR_001785087.1 33.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
696
ORF length:
627
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggcgctg agcggtggcg gtggtggcgg cgcggagccg ggccaggctc
121tgttcaacgg ggacatggag cccgaggccg gcgccggcgc cggcgccgcg gcctcttcgg
181ctgcggaccc tgccattccg gaggaggtgt ggaatatcaa acaaatgatt aagttgacac
241aggaacatat agaggcccta ttggacaaat ttggtgggga gcataatcca ccatcaatat
301atctggaggc ctatgaagaa tacaccagca agctagatgc actccaacaa agagaacaac
361agttattgga atctctgggg aacggaactg atttttctgt ttctagctct gcatcaatgg
421ataccgttac atcttcttcc tcttctagcc tttcagtgct accttcatct ctttcagttt
481ttcaaaatcc cacagatgtg gcacggagca accccaagtc accacaaaaa cctatcgtta
541gagtcttcct gcccaacaaa cagaggacag tggtacctgc aaggtgtgga gttacagtcc
601gagacagtct aaagaaagca ctgatgatga gaggtctaat cccagagtgc tgtgctgttt
661acagaattca tgatgggtct tttctggagc tgacttgaag aaaagagtac atctctttac
721cctgctgttt gtccaagagt gatacattta tttgggagag aagttccact gatgtgtgtt
781aattatgacc aacttgattt gctgtttgtc tccaagttct ttgaacacca cccaatacca
841caggaagagg cgtccttagc agagactgcc ctaacatctg gatcatcccc ttccgcaccc
901gcctcggact ctattgggcc ccaaattctc accagtccgt ctccttcaaa atccattcca
961attccacagc ccttccgacc agcagatgaa gatcatcgaa atcaatttgg gcaacgagac
1021cgatcctcat cagctcccaa tgtgcatata aacacaatag aacctgtcaa tattgatgac
1081ttgattagag accaaggatt tcgtggtgat ggaggatcaa ccacaggttt gtctgctacc
1141ccccctgcct cattacctgg ctcactaact aacgtgaaag ccttacagaa atctccagga
1201cctcagcgag aaaggaagtc atcttcatcc tcagaagaca ggaatcgaat gaaaacactt
1261ggtagacggg actcgagtga tgattgggag attcctgatg ggcagattac agtgggacaa
1321agaattggat ctggatcatt tggaacagtc tacaagggaa agtggcatgg tgatgtggca
1381gtgaaaatgt tgaatgtgac agcacctaca cctcagcagt tacaagcctt caaaaatgaa
1441gtaggagtac tcaggaaaac acgacatgtg aatatcctac tcttcatggg ctattccaca
1501aagccacaac tggctattgt tacccagtcg tgtgaaggac tcccagcttg tatcaccatc
1561tccatatcat tgagaccaaa tttgagatga tcaaacttat agatattgca cgacagactg
1621cacagggcat ggattactta cacgccaagt caatcatcca cagagacctc aagagtaata
1681atatatttct tcatgaagac ctcacagtaa aaataggtga ttttggtcta gctacagtga
1741aatctcgatg gagtgggtcc catcagtttg aacagttgtc tggatccatt ttgtggatgg
1801caccagaagt catcagaatg caagataaaa atccatacag ctttcagtca gatgtatatg
1861catttggaat tgttctgtat gaattgatga ctggacagtt accttattca aacatcaaca
1921acagggacca gataattttt atggtgggac gaggatacct gtctccagat ctcagtaagg
1981tacggagtaa ctgtccaaaa gccatgaaga gattaatggc agagtgcctc aaaaagaaaa
2041gagatgagag accactcttt ccccaaattc tcgcctctat tgagctgctg gcccgctcat
2101tgccaaaaat tcaccgcagt gcatcagaac cctccttgaa tcgggctggt ttccaaacag
2161aggattttag tctatatgct tgtgcttctc caaaaacacc catccaggca gggggatatg
2221gtgcgtttcc tgtccacttg ccaactttct tgtacaaagt ggttggtaag cctatcccta
2281accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
2341agtcgacaat caacctctgg atta

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