Construct: ORF ccsbBroad304_14553
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018930.1_s304c1, BRDN0000459414
- Derived from:
- ccsbBroadEn_14553
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BRAF (673)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14553
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_004333.6 | 93.1% | 26.3% | (many diffs) |
| 2 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001354609.2 | 92.7% | 26.3% | (many diffs) |
| 3 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001374244.1 | 88.5% | 25% | (many diffs) |
| 4 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001374258.1 | 88.1% | 25% | (many diffs) |
| 5 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XM_017012559.1 | 82.9% | 25.3% | (many diffs) |
| 6 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XR_001744857.1 | 44.8% | (many diffs) | |
| 7 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XR_001744858.1 | 20.5% | (many diffs) | |
| 8 | mouse | 109880 | Braf | Braf transforming gene | XM_006505359.3 | 77% | 22.4% | (many diffs) |
| 9 | mouse | 109880 | Braf | Braf transforming gene | XM_006505358.3 | 76.7% | 22.4% | (many diffs) |
| 10 | mouse | 109880 | Braf | Braf transforming gene | XM_011241139.2 | 76.4% | 22.6% | (many diffs) |
| 11 | mouse | 109880 | Braf | Braf transforming gene | XM_011241138.2 | 75.4% | 22.2% | (many diffs) |
| 12 | mouse | 109880 | Braf | Braf transforming gene | NM_139294.5 | 75.4% | 19.3% | (many diffs) |
| 13 | mouse | 109880 | Braf | Braf transforming gene | XM_011241136.2 | 75.1% | 19.4% | (many diffs) |
| 14 | mouse | 109880 | Braf | Braf transforming gene | XM_011241137.1 | 74.5% | 18.8% | (many diffs) |
| 15 | mouse | 109880 | Braf | Braf transforming gene | XM_006505355.3 | 73.3% | 21.4% | (many diffs) |
| 16 | mouse | 109880 | Braf | Braf transforming gene | XM_011241135.2 | 73.1% | 21.6% | (many diffs) |
| 17 | mouse | 109880 | Braf | Braf transforming gene | XM_006505353.3 | 72.4% | 21.1% | (many diffs) |
| 18 | mouse | 109880 | Braf | Braf transforming gene | XM_011241134.2 | 72.1% | 21.3% | (many diffs) |
| 19 | mouse | 109880 | Braf | Braf transforming gene | XM_011241140.2 | 51.7% | 27.2% | (many diffs) |
| 20 | mouse | 109880 | Braf | Braf transforming gene | XR_001785085.1 | 38.3% | (many diffs) | |
| 21 | mouse | 109880 | Braf | Braf transforming gene | XR_001785086.1 | 34.9% | (many diffs) | |
| 22 | mouse | 109880 | Braf | Braf transforming gene | XR_001785087.1 | 33.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 696
- ORF length:
- 627
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgctg agcggtggcg gtggtggcgg cgcggagccg ggccaggctc 121 tgttcaacgg ggacatggag cccgaggccg gcgccggcgc cggcgccgcg gcctcttcgg 181 ctgcggaccc tgccattccg gaggaggtgt ggaatatcaa acaaatgatt aagttgacac 241 aggaacatat agaggcccta ttggacaaat ttggtgggga gcataatcca ccatcaatat 301 atctggaggc ctatgaagaa tacaccagca agctagatgc actccaacaa agagaacaac 361 agttattgga atctctgggg aacggaactg atttttctgt ttctagctct gcatcaatgg 421 ataccgttac atcttcttcc tcttctagcc tttcagtgct accttcatct ctttcagttt 481 ttcaaaatcc cacagatgtg gcacggagca accccaagtc accacaaaaa cctatcgtta 541 gagtcttcct gcccaacaaa cagaggacag tggtacctgc aaggtgtgga gttacagtcc 601 gagacagtct aaagaaagca ctgatgatga gaggtctaat cccagagtgc tgtgctgttt 661 acagaattca tgatgggtct tttctggagc tgacttgaag aaaagagtac atctctttac 721 cctgctgttt gtccaagagt gatacattta tttgggagag aagttccact gatgtgtgtt 781 aattatgacc aacttgattt gctgtttgtc tccaagttct ttgaacacca cccaatacca 841 caggaagagg cgtccttagc agagactgcc ctaacatctg gatcatcccc ttccgcaccc 901 gcctcggact ctattgggcc ccaaattctc accagtccgt ctccttcaaa atccattcca 961 attccacagc ccttccgacc agcagatgaa gatcatcgaa atcaatttgg gcaacgagac 1021 cgatcctcat cagctcccaa tgtgcatata aacacaatag aacctgtcaa tattgatgac 1081 ttgattagag accaaggatt tcgtggtgat ggaggatcaa ccacaggttt gtctgctacc 1141 ccccctgcct cattacctgg ctcactaact aacgtgaaag ccttacagaa atctccagga 1201 cctcagcgag aaaggaagtc atcttcatcc tcagaagaca ggaatcgaat gaaaacactt 1261 ggtagacggg actcgagtga tgattgggag attcctgatg ggcagattac agtgggacaa 1321 agaattggat ctggatcatt tggaacagtc tacaagggaa agtggcatgg tgatgtggca 1381 gtgaaaatgt tgaatgtgac agcacctaca cctcagcagt tacaagcctt caaaaatgaa 1441 gtaggagtac tcaggaaaac acgacatgtg aatatcctac tcttcatggg ctattccaca 1501 aagccacaac tggctattgt tacccagtcg tgtgaaggac tcccagcttg tatcaccatc 1561 tccatatcat tgagaccaaa tttgagatga tcaaacttat agatattgca cgacagactg 1621 cacagggcat ggattactta cacgccaagt caatcatcca cagagacctc aagagtaata 1681 atatatttct tcatgaagac ctcacagtaa aaataggtga ttttggtcta gctacagtga 1741 aatctcgatg gagtgggtcc catcagtttg aacagttgtc tggatccatt ttgtggatgg 1801 caccagaagt catcagaatg caagataaaa atccatacag ctttcagtca gatgtatatg 1861 catttggaat tgttctgtat gaattgatga ctggacagtt accttattca aacatcaaca 1921 acagggacca gataattttt atggtgggac gaggatacct gtctccagat ctcagtaagg 1981 tacggagtaa ctgtccaaaa gccatgaaga gattaatggc agagtgcctc aaaaagaaaa 2041 gagatgagag accactcttt ccccaaattc tcgcctctat tgagctgctg gcccgctcat 2101 tgccaaaaat tcaccgcagt gcatcagaac cctccttgaa tcgggctggt ttccaaacag 2161 aggattttag tctatatgct tgtgcttctc caaaaacacc catccaggca gggggatatg 2221 gtgcgtttcc tgtccacttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 2281 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2341 agtcgacaat caacctctgg atta