Construct: ORF ccsbBroadEn_14553
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018930.1_s300c1, BRDN0000458226
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BRAF (673)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_004333.6 | 93.1% | 26.3% | (many diffs) |
| 2 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001354609.2 | 92.7% | 26.3% | (many diffs) |
| 3 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001374244.1 | 88.5% | 25% | (many diffs) |
| 4 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001374258.1 | 88.1% | 25% | (many diffs) |
| 5 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XM_017012559.1 | 82.9% | 25.3% | (many diffs) |
| 6 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XR_001744857.1 | 44.8% | (many diffs) | |
| 7 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XR_001744858.1 | 20.5% | (many diffs) | |
| 8 | mouse | 109880 | Braf | Braf transforming gene | XM_006505359.3 | 77% | 22.4% | (many diffs) |
| 9 | mouse | 109880 | Braf | Braf transforming gene | XM_006505358.3 | 76.7% | 22.4% | (many diffs) |
| 10 | mouse | 109880 | Braf | Braf transforming gene | XM_011241139.2 | 76.4% | 22.6% | (many diffs) |
| 11 | mouse | 109880 | Braf | Braf transforming gene | XM_011241138.2 | 75.4% | 22.2% | (many diffs) |
| 12 | mouse | 109880 | Braf | Braf transforming gene | NM_139294.5 | 75.4% | 19.3% | (many diffs) |
| 13 | mouse | 109880 | Braf | Braf transforming gene | XM_011241136.2 | 75.1% | 19.4% | (many diffs) |
| 14 | mouse | 109880 | Braf | Braf transforming gene | XM_011241137.1 | 74.5% | 18.8% | (many diffs) |
| 15 | mouse | 109880 | Braf | Braf transforming gene | XM_006505355.3 | 73.3% | 21.4% | (many diffs) |
| 16 | mouse | 109880 | Braf | Braf transforming gene | XM_011241135.2 | 73.1% | 21.6% | (many diffs) |
| 17 | mouse | 109880 | Braf | Braf transforming gene | XM_006505353.3 | 72.4% | 21.1% | (many diffs) |
| 18 | mouse | 109880 | Braf | Braf transforming gene | XM_011241134.2 | 72.1% | 21.3% | (many diffs) |
| 19 | mouse | 109880 | Braf | Braf transforming gene | XM_011241140.2 | 51.7% | 27.2% | (many diffs) |
| 20 | mouse | 109880 | Braf | Braf transforming gene | XR_001785085.1 | 38.3% | (many diffs) | |
| 21 | mouse | 109880 | Braf | Braf transforming gene | XR_001785086.1 | 34.9% | (many diffs) | |
| 22 | mouse | 109880 | Braf | Braf transforming gene | XR_001785087.1 | 33.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 725
- ORF length:
- 627
- Sequence:
-
1 AAAGGATTTT TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GCGGCGCTGA GCGGTGGCGG 121 TGGTGGCGGC GCGGAGCCGG GCCAGGCTCT GTTCAACGGG GACATGGAGC CCGAGGCCGG 181 CGCCGGCGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCT GCCATTCCGG AGGAGGTGTG 241 GAATATCAAA CAAATGATTA AGTTGACACA GGAACATATA GAGGCCCTAT TGGACAAATT 301 TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC TATGAAGAAT ACACCAGCAA 361 GCTAGATGCA CTCCAACAAA GAGAACAACA GTTATTGGAA TCTCTGGGGA ACGGAACTGA 421 TTTTTCTGTT TCTAGCTCTG CATCAATGGA TACCGTTACA TCTTCTTCCT CTTCTAGCCT 481 TTCAGTGCTA CCTTCATCTC TTTCAGTTTT TCAAAATCCC ACAGATGTGG CACGGAGCAA 541 CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG CCCAACAAAC AGAGGACAGT 601 GGTACCTGCA AGGTGTGGAG TTACAGTCCG AGACAGTCTA AAGAAAGCAC TGATGATGAG 661 AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAT GATGGGTCTT TTCTGGAGCT 721 GACTTGAAGA AAAGAGTACA TCTCTTTACC CTGCTGTTTG TCCAAGAGTG ATACATTTAT 781 TTGGGAGAGA AGttccactg atgtgtgtta attatgacca acttgatttg ctgtttgtct 841 ccaagttctt tgaacaccac ccaataccac aggaagaggc gtccttagca gagactgccc 901 taacatctgg atcatcccct tccgcacccg cctcggactc tattgggccc caaattctca 961 ccagtccgtc tccttcaaaa tccattccaa ttccacagcc cttccgacca gcagatgaag 1021 atcatcgaaa tcaatttggg caacgagacc gatcctcatc agctcccaat gtgcatataa 1081 acacaataga acctgtcaat attgatgact tgattagaga ccaaggattt cgtggtgatg 1141 gaggatcaac cacaggtttg tctgctaccc cccctgcctc attacctggc tcactaacta 1201 acgtgaaagc cttacagaaa tctccaggac ctcagcgaga aaggaagtca tcttcatcct 1261 cagaagacag gaatcgaatg aaaacacttg gtagacggga ctcgagtgat gattgggaga 1321 ttcctgatgg gcagattaca gtgggacaaa gaattggatc tggatcattt ggaacagtct 1381 acaagggaaa gtggcatggt gatgtggcag tgaaaatgtt gaatgtgaca gcacctacac 1441 ctcagcagtt acaagccttc aaaaatgaag taggagtact caggaaaaca cgacatgtga 1501 atatcctact cttcatgggc tattccacaa agccacaact ggctattgtt acccagtCGT 1561 GTGAAGGACT CCCAGCTTGT ATCACCATCT CCATATCATT GAGACCAAAT TTGAGATGAT 1621 CAAACTTATA GATATTGCAC GACAGACTGC ACAGGGCATG GATTACTTAC ACGCCAAGTC 1681 AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT CATGAAGACC TCACAGTAAA 1741 AATAGGTGAT TTTGGTCTAG CTACAGTGAA ATCTCGATGG AGTGGGTCCC ATCAGTTTGA 1801 ACAGTTGTCT GGATCCATTT TGTGGATGGC ACCAGAAGTC ATCAGAATGC AAGATAAAAA 1861 TCCATACAGC TTTCAGTCAG ATGTATATGC ATTTGGAATT GTTCTGTATG AATTGATGAC 1921 TGGACAGTTA CCTTATTCAA ACATCAACAA CAGGGACCAG ATAATTTTTA TGGTGGGACG 1981 AGGATACCTG TCTCCAGATC TCAGTAAGGT ACGGAGTAAC TGTCCAAAAG CCATGAAGAG 2041 ATTAATGGCA GAGTGCCTCA AAAAGAAAAG AGATGAGAGA CCACTCTTTC CCCAAATTCT 2101 CGCCTCTATT GAGCTGCTGG CCCGCTCATT GCCAAAAATT CACCGCAGTG CATCAGAACC 2161 CTCCTTGAAT CGGGCTGGTT TCCAAACAGA GGATTTTAGT CTATATGCTT GTGCTTCTCC 2221 AAAAACACCC ATCCAGGCAG GGGGATATGG TGCGTTTCCT GTCCACTTGC CAACTTTCTT 2281 GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC 2341 TATCAGTCAA AATAAAATCA TATTCCATCC AGCTAGGCTC AG