Construct: ORF ccsbBroadEn_14553

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018930.1_s300c1, BRDN0000458226
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
BRAF (673)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 673 BRAF B-Raf proto-oncogene, serin... NM_004333.6 93.1% 26.3% (many diffs)
2 human 673 BRAF B-Raf proto-oncogene, serin... NM_001354609.2 92.7% 26.3% (many diffs)
3 human 673 BRAF B-Raf proto-oncogene, serin... NM_001374244.1 88.5% 25% (many diffs)
4 human 673 BRAF B-Raf proto-oncogene, serin... NM_001374258.1 88.1% 25% (many diffs)
5 human 673 BRAF B-Raf proto-oncogene, serin... XM_017012559.1 82.9% 25.3% (many diffs)
6 human 673 BRAF B-Raf proto-oncogene, serin... XR_001744857.1 44.8% (many diffs)
7 human 673 BRAF B-Raf proto-oncogene, serin... XR_001744858.1 20.5% (many diffs)
8 mouse 109880 Braf Braf transforming gene XM_006505359.3 77% 22.4% (many diffs)
9 mouse 109880 Braf Braf transforming gene XM_006505358.3 76.7% 22.4% (many diffs)
10 mouse 109880 Braf Braf transforming gene XM_011241139.2 76.4% 22.6% (many diffs)
11 mouse 109880 Braf Braf transforming gene XM_011241138.2 75.4% 22.2% (many diffs)
12 mouse 109880 Braf Braf transforming gene NM_139294.5 75.4% 19.3% (many diffs)
13 mouse 109880 Braf Braf transforming gene XM_011241136.2 75.1% 19.4% (many diffs)
14 mouse 109880 Braf Braf transforming gene XM_011241137.1 74.5% 18.8% (many diffs)
15 mouse 109880 Braf Braf transforming gene XM_006505355.3 73.3% 21.4% (many diffs)
16 mouse 109880 Braf Braf transforming gene XM_011241135.2 73.1% 21.6% (many diffs)
17 mouse 109880 Braf Braf transforming gene XM_006505353.3 72.4% 21.1% (many diffs)
18 mouse 109880 Braf Braf transforming gene XM_011241134.2 72.1% 21.3% (many diffs)
19 mouse 109880 Braf Braf transforming gene XM_011241140.2 51.7% 27.2% (many diffs)
20 mouse 109880 Braf Braf transforming gene XR_001785085.1 38.3% (many diffs)
21 mouse 109880 Braf Braf transforming gene XR_001785086.1 34.9% (many diffs)
22 mouse 109880 Braf Braf transforming gene XR_001785087.1 33.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
98
ORF end:
725
ORF length:
627
Sequence:
1AAAGGATTTT TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT
61TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GCGGCGCTGA GCGGTGGCGG
121TGGTGGCGGC GCGGAGCCGG GCCAGGCTCT GTTCAACGGG GACATGGAGC CCGAGGCCGG
181CGCCGGCGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCT GCCATTCCGG AGGAGGTGTG
241GAATATCAAA CAAATGATTA AGTTGACACA GGAACATATA GAGGCCCTAT TGGACAAATT
301TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC TATGAAGAAT ACACCAGCAA
361GCTAGATGCA CTCCAACAAA GAGAACAACA GTTATTGGAA TCTCTGGGGA ACGGAACTGA
421TTTTTCTGTT TCTAGCTCTG CATCAATGGA TACCGTTACA TCTTCTTCCT CTTCTAGCCT
481TTCAGTGCTA CCTTCATCTC TTTCAGTTTT TCAAAATCCC ACAGATGTGG CACGGAGCAA
541CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG CCCAACAAAC AGAGGACAGT
601GGTACCTGCA AGGTGTGGAG TTACAGTCCG AGACAGTCTA AAGAAAGCAC TGATGATGAG
661AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAT GATGGGTCTT TTCTGGAGCT
721GACTTGAAGA AAAGAGTACA TCTCTTTACC CTGCTGTTTG TCCAAGAGTG ATACATTTAT
781TTGGGAGAGA AGttccactg atgtgtgtta attatgacca acttgatttg ctgtttgtct
841ccaagttctt tgaacaccac ccaataccac aggaagaggc gtccttagca gagactgccc
901taacatctgg atcatcccct tccgcacccg cctcggactc tattgggccc caaattctca
961ccagtccgtc tccttcaaaa tccattccaa ttccacagcc cttccgacca gcagatgaag
1021atcatcgaaa tcaatttggg caacgagacc gatcctcatc agctcccaat gtgcatataa
1081acacaataga acctgtcaat attgatgact tgattagaga ccaaggattt cgtggtgatg
1141gaggatcaac cacaggtttg tctgctaccc cccctgcctc attacctggc tcactaacta
1201acgtgaaagc cttacagaaa tctccaggac ctcagcgaga aaggaagtca tcttcatcct
1261cagaagacag gaatcgaatg aaaacacttg gtagacggga ctcgagtgat gattgggaga
1321ttcctgatgg gcagattaca gtgggacaaa gaattggatc tggatcattt ggaacagtct
1381acaagggaaa gtggcatggt gatgtggcag tgaaaatgtt gaatgtgaca gcacctacac
1441ctcagcagtt acaagccttc aaaaatgaag taggagtact caggaaaaca cgacatgtga
1501atatcctact cttcatgggc tattccacaa agccacaact ggctattgtt acccagtCGT
1561GTGAAGGACT CCCAGCTTGT ATCACCATCT CCATATCATT GAGACCAAAT TTGAGATGAT
1621CAAACTTATA GATATTGCAC GACAGACTGC ACAGGGCATG GATTACTTAC ACGCCAAGTC
1681AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT CATGAAGACC TCACAGTAAA
1741AATAGGTGAT TTTGGTCTAG CTACAGTGAA ATCTCGATGG AGTGGGTCCC ATCAGTTTGA
1801ACAGTTGTCT GGATCCATTT TGTGGATGGC ACCAGAAGTC ATCAGAATGC AAGATAAAAA
1861TCCATACAGC TTTCAGTCAG ATGTATATGC ATTTGGAATT GTTCTGTATG AATTGATGAC
1921TGGACAGTTA CCTTATTCAA ACATCAACAA CAGGGACCAG ATAATTTTTA TGGTGGGACG
1981AGGATACCTG TCTCCAGATC TCAGTAAGGT ACGGAGTAAC TGTCCAAAAG CCATGAAGAG
2041ATTAATGGCA GAGTGCCTCA AAAAGAAAAG AGATGAGAGA CCACTCTTTC CCCAAATTCT
2101CGCCTCTATT GAGCTGCTGG CCCGCTCATT GCCAAAAATT CACCGCAGTG CATCAGAACC
2161CTCCTTGAAT CGGGCTGGTT TCCAAACAGA GGATTTTAGT CTATATGCTT GTGCTTCTCC
2221AAAAACACCC ATCCAGGCAG GGGGATATGG TGCGTTTCCT GTCCACTTGC CAACTTTCTT
2281GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC
2341TATCAGTCAA AATAAAATCA TATTCCATCC AGCTAGGCTC AG

Download FASTA (ORF) (Full)

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