Construct: ORF ccsbBroad304_14743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018880.1_s304c1, BRDN0000459364
- Derived from:
- ccsbBroadEn_14743
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PFKFB2 (5208)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14743
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | NM_006212.2 | 98.6% | 48.8% | (many diffs) |
| 2 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_024447654.1 | 98.6% | 48.8% | (many diffs) |
| 3 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_024447655.1 | 98.6% | 48.8% | (many diffs) |
| 4 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_024447656.1 | 92.1% | 42.3% | (many diffs) |
| 5 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | NM_001018053.2 | 90.4% | 52.3% | (many diffs) |
| 6 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_024447657.1 | 90.4% | 52.3% | (many diffs) |
| 7 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_005273162.3 | 51% | 13.2% | (many diffs) |
| 8 | mouse | 18640 | Pfkfb2 | 6-phosphofructo-2-kinase/fr... | NM_008825.4 | 86.4% | 43.5% | (many diffs) |
| 9 | mouse | 18640 | Pfkfb2 | 6-phosphofructo-2-kinase/fr... | NM_001162416.1 | 82.7% | 45.4% | (many diffs) |
| 10 | mouse | 18640 | Pfkfb2 | 6-phosphofructo-2-kinase/fr... | NM_001162415.1 | 80.6% | 47.6% | (many diffs) |
| 11 | mouse | 18640 | Pfkfb2 | 6-phosphofructo-2-kinase/fr... | NR_027859.1 | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 879
- ORF length:
- 810
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctggggca tcttcctcag aacagaacaa caacagctat gaaaccaaaa 121 ccccaaatct tcgaatgtca gagaagaaat gttcatgggc ctcctacatg accaactccc 181 cgactctgat cgttatgatt ggtttgccag cccggggtaa aacctacgtg tccaagaaac 241 taacacgcta cctcaactgg attggagtcc ccaccaaagt gtttaatctt ggggtgtatc 301 ggcgtgaagc agtcaagtcc tataagtcct acgacttctt tcggcatgac aatgaggagg 361 ccatgaagat ccgcaaacag tgtgctctgg tggcgctgga agatgttaag gcgtatctca 421 ctgaggagaa tggtcagatt gcggtgtttg atgccaccaa tacaacccgg gagaggaggg 481 acatgatttt gaactttgct gaacagaatt ccttcaaggt attctttgtg gaatccgtct 541 gtgatgatcc tgatgtcatt gctgccaata ttctggaggt taaggtatca agccctgact 601 atcctgaaag gaatagagag aacgtgatgg aggacttcct gaagagaatt gaatgctaca 661 aagttaccta ccgacctctt gacccagaca actatgacaa ggatctttct ttcatcaaag 721 tgataaacgt gggccagcga tttttagtca acagagtcca ggactacatc cagagccaga 781 tagtctacta cctcatgaan atccacgtcc ancnctcgca ccatttacct ttnncggcat 841 ggannaagcg agttcnatct cttgggnaan atngggggtg actctggcct cctcggtgcg 901 gggnaagcag tttgcccaag ctctaaggaa atttctgagg gaacaggaaa taacagacct 961 caaagtgtgg acaagccagt gnaagaggac catacagact gctgaatctc tcggggtgcc 1021 ctatgagcag tggaagattc tgaatgagat tgatgctggt gtgtgtgaag agatgaccta 1081 tgcagagatt gagaaacggt acccagaaga gtttgcactt cgagatcaag agaagtatct 1141 gtatcgatat cctggtgggg agtcatacca ggacctggtg cagcggctgg agcctgtcat 1201 catggagctg gaacgtcagg gcaatgtcct cgtcatctcc caccaggctg tcatgcgctg 1261 cctcctggcc tacttcttgg ataagggcgc agatgagcta ccatacttga gatgccctct 1321 ccataccatc ttcaaactta ctcctgtggc ctatgggtgc aaagtggaaa caattaaact 1381 taacgtggag gctgtgaaca cgcaccgtga caagccaact aacaacttcc ccaagaacca 1441 aacccctgta aggatgagaa ggaacagctt tacgcctctg tccagttcga atacaataag 1501 gcgtccaaga aattacagtg ttgggagccg gcccctcaag cccctcagcc ctctccgtgc 1561 ccaggacatg caagaagggg ccgacttgcc aactttcttg tacaaagtgg ttggtaagcc 1621 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg 1681 gcgcgttaag tcgacaatca acctctggat ta