Construct: ORF ccsbBroad304_14764

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018977.1_s304c1, BRDN0000459460
Derived from:
ccsbBroadEn_14764
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PI4KA (5297)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14764
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5297 PI4KA phosphatidylinositol 4-kina... XM_017028830.1 57.5% 15.1% (many diffs)
2 human 728233 PI4KAP1 phosphatidylinositol 4-kina... NR_003563.1 52.9% (many diffs)
3 human 375133 PI4KAP2 phosphatidylinositol 4-kina... NR_003700.1 50.4% (many diffs)
4 human 5297 PI4KA phosphatidylinositol 4-kina... NM_001362862.1 40.8% 10.7% (many diffs)
5 human 5297 PI4KA phosphatidylinositol 4-kina... NM_001362863.1 40.6% 10.7% (many diffs)
6 human 5297 PI4KA phosphatidylinositol 4-kina... NM_058004.4 40.2% 10.6% (many diffs)
7 human 5297 PI4KA phosphatidylinositol 4-kina... XR_937868.1 35.8% (many diffs)
8 human 5297 PI4KA phosphatidylinositol 4-kina... XM_005261635.1 26.9% 10.4% (many diffs)
9 mouse 224020 Pi4ka phosphatidylinositol 4-kina... XM_006522037.2 64.6% 18.6% (many diffs)
10 mouse 224020 Pi4ka phosphatidylinositol 4-kina... NM_001001983.2 37% 10.7% (many diffs)
11 mouse 224020 Pi4ka phosphatidylinositol 4-kina... XM_006522034.1 36% 10.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
777
ORF length:
711
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg ggagatggca ggggcctggc acatgacggt ggagcagaaa tttggcctgt
121tttctgctga gataaaggaa gcagaccccc tggctgcctc ggaagcaagt caacccaaac
181cctgtccccc cgaagtgacc ccccactaca tctggatcga cttcctggtg cagcggtttg
241agatcgccaa gtactgcagc tctgaccaag tggagatctt ctccagcctg ctgcagcgct
301ccatgtccct gaacatcggc ggggccaagg ggagcatgaa ccggcacgtg gcggccatcg
361ggccccgctt caagctgctg accctggggc tgtccctcct gcatgccgat gtggttccaa
421atgcaaccat ccgcaatgtg cttcgcgaga agatctactc cactgccttt gactacttca
481gctgtccccc aaagttccct actcaaggag agaagcggct gcgtgaagac ataagcatca
541tgattaaatt ttggaccgcc atgttctcag ataagaagta cctgaccgcc agccagcttg
601ttcccccaga taatcaggac acccggagca acctggacat aactgtcggc tctcggcaac
661aagccaccca aggctggatc aacacatacc ccctgtccag cggcatgtcc accatctcca
721agaaatcagc atgtctaaga aaaccaaccg gggctcccag ctgcacaaat actacatgaa
781gcgcagacgc tgctgctgtc cctgctggcc actgagatcg agcgtctcat cacatggtac
841acccgctgtc agccccggaa ctggaactag acaaggccga aaaagaacag ccgtggccac
901tgggagattc taagtacatc agcctgagtg agaagcagtg gaaggacaac gtgaacctcg
961cctggagcat ctctccctac ctagccgtgc agctgcctgc caggtttaag aacacagaag
1021ccattgggaa cgaagtgacc cgtctcgttc ggttggaccc gggagccgtt agtgatgtgc
1081ctgaagcaat caagttcctg gtcacctggc acaccatcga cgccgatgct ccagagctca
1141gccatgtgct gtgctgggcg cccacggacc cacccacagg cctctcctac ttctccagca
1201tgtacccgcc gcaccctctc acggcgcagt acggggtgaa agtcctgcgg tccttccctc
1261cggacgccat cctcttctac atcccccaga ttgtgcaggc cctcaggtac gacaagatgg
1321gctatgtgcg ggagtatatt ctgtgggcag cgtctaaatc ccagcttctg gcacaccagt
1381tcatctggaa catgaagact aacatttatc tagatgaaga gggccaccag aaagaccctg
1441acatcggcga cctcctggat cagttggtag aggagatcac aggctccttg tccggcccag
1501cgaaggactt ttaccagcgg gagtttgatt tctttaacaa gatcaccaac gtgtcggcta
1561tcatcaagcc ctaccctaaa ggcgacgaga gaaagaaggc ttgtctgtcg gccctgtctg
1621aagtgaaggt gcagccgggc tgctacctgc ccagcaaccc tgaggccatt gtgctggaca
1681tcgactacaa gtctgggacc ccgatgcaga gtgctgcaaa agccccatat ctggccaagt
1741tcaaggtgaa gcgatgtgga gttagtgaac ttgaaaaaga aggtctgcgg tgccgctcag
1801actccgagga tgagtcagca agcagaagcc cgacgccaga aagatttctg gcaggcagca
1861tcttcaaagg tgggagacga ctgccggcaa gacatgctgg ccctgcagat catcgacctc
1921ntcaaagaac atctcccagg ctgtcggcct ggacctcttt gtttttcccn taccgcgtgg
1981tggccactgc ccctgggtgc ggggtgatcg agtgcatccc cgactgcacc tcccgggacc
2041agctgggccg ccagacagac ttcggcatgt acgactactt cacacgccag tacggggatg
2101agtccactct ggccttccag caggcccgct acaacttcat ccgaagcatg gccgcctaca
2161gcctcctgct gttcctgctg cagatcaagg acagacacaa cggcaacatt atgctggaca
2221agaagggtca tatcatccac atcgactttg gcttcatgtt tgaaagctcg ccgggcggca
2281atctcggctg ggaacccgac atcaagctga cggatgagat ggtgatgatc atggggggca
2341agatggaggc cacacccttc aagtggttca tggagatgtg tgtccgaggc tacctggctg
2401tgcggcccta catggacgcg gtcgtctccc tggtcactct catgttggac acgggcctgc
2461cctgttttcg cggccagaca atcaagctct tgaagcacag gtttagcccc aacatgactg
2521agcgcgaggc tgcaaatttc atcatgaagg tcatccagag ctgcttcctc agcaacagga
2581gccggaccta cgacatgatc cagtactatc agaatgacat cccctacttg ccaactttct
2641tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
2701aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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