Construct: ORF ccsbBroad304_14807
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018842.1_s304c1, BRDN0000459326
- Derived from:
- ccsbBroadEn_14807
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP2K1 (5604)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14807
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5604 | MAP2K1 | mitogen-activated protein k... | NM_002755.3 | 99.9% | 36.3% | 413_414insG |
| 2 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_011521783.3 | 93.6% | 29.5% | (many diffs) |
| 3 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_017022411.2 | 93.3% | 39.2% | 413_414insG;438_439ins78 |
| 4 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_017022412.1 | 87% | 31.8% | (many diffs) |
| 5 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_017022413.1 | 53.3% | 1.6% | (many diffs) |
| 6 | mouse | 26395 | Map2k1 | mitogen-activated protein k... | NM_008927.3 | 90.5% | 36.3% | (many diffs) |
| 7 | mouse | 26395 | Map2k1 | mitogen-activated protein k... | XM_006511196.3 | 84.7% | 29.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 555
- ORF length:
- 486
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccaagaag aagccgacgc ccatccagct gaacccggcc cccgacggct 121 ctgcagttaa cgggaccagc tctgcggaga ccaacttgga ggccttgcag aagaagctgg 181 aggagctaga gcttgatgag cagcagcgaa agcgccttga ggcctttctt acccagaagc 241 agaaggtggg agaactgaag gatgacgact ttgagaagat cagtgagctg ggggctggca 301 atggcggtgt ggtgttcaag gtctcccaca agccttctgg cctggtcatg gccagaaagc 361 taattcatct ggagatcaaa cccgcaatcc ggaaccagat cataagggag ctgcaggttc 421 tgcatgagtg caactctccg tacatcgtgg gcttctatgg tgcgttctac agcgatggcg 481 aggatcagta tctgcatgga gcacatggat ggaggttctc tggatcaagt cctgaagaaa 541 gctggaagaa ttcctgaaca aattttagga aaagttagca ttgctgtaat aaaaggcctg 601 acatatctga gggagaagca caagatcatg cacagagatg tcaagcccTC CAACATCCTA 661 GTCAACTCCC GTGGGGAGAT CAAGCTCTGT GACTTTGGGG TCAGCGGGCA GCTCATCGAC 721 TCCATGGCCA ACTCCTTCGT GGGCACAAGG TCCTACATGT CGCCAGAAAG ACTCCAGGGG 781 ACTCATTACT CTGTGCAGTC AGACATCTGG AGCATGGGAC TGTCTCTGGT AGAGATGGCG 841 GTTGGGAGGT ATCCCATCCC TCCTCCAGAT GCCAAGGAGC TGGAGCTGAT GTTTGGGTGC 901 CAGGTGGAAG GAGATGCGGC TGAGACCCCA CCCAGGCCAA GGACCCCCGG GAGGCCCCTT 961 AGCTCATACG GAATGGACAG CCGACCTCCC ATGGCAATTT TTGAGTTGTT GGATTACATA 1021 GTCAACGAGC CTCCTCCAAA ACTGCCCAGT GGAGTGTTCA GTCTGGAATT TCAAGATTTT 1081 GTGAATAAAT GCTTAATAAA AAACCCCGCA GAGAGAGCAG ATTTGAAGCA ACTCATGGTT 1141 CATGCTTTTA TCAAGAGATC TGATGCTGAG GAAGTGGATT TTGCAGGTTG GCTCTGCTCC 1201 ACCATCGGCC TTAACCAGCC CAGCACACCA ACCCATGCTG CTGGCGTCTT GCCAACTTTC 1261 TTGTACaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag 1321 taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta