Construct: ORF ccsbBroad304_14984

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010680.2_s304c1, BRDN0000459546
Derived from:
ccsbBroadEn_14984
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TLK2 (11011)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14984
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11011 TLK2 tousled like kinase 2 NM_001284333.2 99.8% 99.8% 1_3delATG
2 human 11011 TLK2 tousled like kinase 2 XM_011524216.2 99.8% 99.8% 1_3delATG
3 human 11011 TLK2 tousled like kinase 2 XM_011524217.3 99.8% 99.8% 1_3delATG
4 human 11011 TLK2 tousled like kinase 2 XM_011524222.3 99.8% 99.8% 1_3delATG
5 human 11011 TLK2 tousled like kinase 2 XM_024450553.1 99.8% 99.8% 1_3delATG
6 human 11011 TLK2 tousled like kinase 2 XM_024450552.1 98.9% 98.9% 1_24del
7 human 11011 TLK2 tousled like kinase 2 NM_006852.4 97% 97% 1_3delATG;1121_1122ins66
8 human 11011 TLK2 tousled like kinase 2 XM_017024044.1 97% 97% 1_3delATG;1121_1122ins66
9 human 11011 TLK2 tousled like kinase 2 XM_017024046.1 97% 97% 1_3delATG;1121_1122ins66
10 human 11011 TLK2 tousled like kinase 2 XM_017024047.1 97% 97% 1_3delATG;1121_1122ins66
11 human 11011 TLK2 tousled like kinase 2 XM_011524215.2 95.8% 95.8% 1_3delATG;627_628ins93
12 human 11011 TLK2 tousled like kinase 2 XM_011524221.2 95.7% 95.7% 1_3delATG;266_267ins96
13 human 11011 TLK2 tousled like kinase 2 XM_017024048.2 95.7% 95.7% 1_3delATG;266_267ins96
14 human 11011 TLK2 tousled like kinase 2 XM_017024049.2 95.7% 95.7% 1_3delATG;266_267ins96
15 human 11011 TLK2 tousled like kinase 2 XM_011524218.2 93% 93% 1_3delATG;627_628ins93;1028_1029ins66
16 human 11011 TLK2 tousled like kinase 2 NM_001284363.1 92.8% 92.8% 1_3delATG;266_267ins96;1025_1026ins66
17 human 11011 TLK2 tousled like kinase 2 XM_017024051.2 92.8% 92.8% 1_3delATG;266_267ins96;1025_1026ins66
18 human 11011 TLK2 tousled like kinase 2 XM_017024052.2 92.8% 92.8% 1_3delATG;266_267ins96;1025_1026ins66
19 human 11011 TLK2 tousled like kinase 2 XM_017024053.2 92.8% 92.8% 1_3delATG;266_267ins96;1025_1026ins66
20 human 11011 TLK2 tousled like kinase 2 XM_011524220.2 91.7% 91.7% 1_3delATG;530_531ins189
21 human 11011 TLK2 tousled like kinase 2 XM_011524223.3 91.7% 91.7% 1_3delATG;266_267ins96;531_532ins93
22 human 11011 TLK2 tousled like kinase 2 XM_017024054.2 88.8% 88.8% 1_3delATG;530_531ins189;932_933ins66
23 human 11011 TLK2 tousled like kinase 2 XM_017024045.1 87.6% 87.6% 1_141del;404_405ins96;1163_1164ins66
24 human 11011 TLK2 tousled like kinase 2 XM_011524224.3 86.6% 84.5% 0_1ins169;50_51ins140
25 human 11011 TLK2 tousled like kinase 2 XM_011524225.3 86.6% 84.5% 0_1ins169;50_51ins140
26 human 11011 TLK2 tousled like kinase 2 XM_011524226.2 86.6% 84.5% 0_1ins169;50_51ins140
27 human 11011 TLK2 tousled like kinase 2 XM_011524227.2 86.6% 84.5% 0_1ins169;50_51ins140
28 human 11011 TLK2 tousled like kinase 2 XM_011524228.3 86.6% 84.5% 0_1ins169;50_51ins140
29 human 11011 TLK2 tousled like kinase 2 XM_017024055.2 84.7% 84.7% (many diffs)
30 human 11011 TLK2 tousled like kinase 2 XM_017024050.1 83.8% 83.8% (many diffs)
31 human 11011 TLK2 tousled like kinase 2 XM_017024056.2 83.7% 81.7% 0_1ins169;50_51ins140;809_810ins66
32 human 11011 TLK2 tousled like kinase 2 XM_017024057.1 83.7% 81.7% 0_1ins169;50_51ins140;809_810ins66
33 human 11011 TLK2 tousled like kinase 2 XM_011524229.2 80.8% 80.8% 0_1ins444
34 human 11011 TLK2 tousled like kinase 2 NM_001330418.2 77.9% 77.9% 0_1ins444;674_675ins66
35 human 11011 TLK2 tousled like kinase 2 XM_017024059.1 77.9% 77.9% 0_1ins444;674_675ins66
36 human 11011 TLK2 tousled like kinase 2 XM_017024060.1 69.7% 69.7% 0_1ins444;83_84ins189;485_486ins66
37 human 11011 TLK2 tousled like kinase 2 XM_011524231.1 66.6% 65.9% (many diffs)
38 human 101928451 LOC101928451 uncharacterized LOC101928451 XR_927955.2 41.7% (many diffs)
39 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001112705.2 90.8% 96.3% (many diffs)
40 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533312.3 90.8% 96.3% (many diffs)
41 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533313.3 90.8% 96.3% (many diffs)
42 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_017314550.1 88.5% 93.9% (many diffs)
43 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294331.1 86.7% 92.3% (many diffs)
44 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_011249014.2 86.7% 92.3% (many diffs)
45 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533314.3 84.8% 89.8% (many diffs)
46 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533315.3 84.5% 90% (many diffs)
47 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533316.3 84% 89.2% (many diffs)
48 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_017314552.1 82.9% 88.2% (many diffs)
49 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294334.1 82.5% 83.6% (many diffs)
50 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_017314551.1 80.9% 85.9% (many diffs)
51 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294329.1 78.2% 81.4% (many diffs)
52 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533320.3 78.2% 81.4% (many diffs)
53 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294330.1 74.7% 75.6% (many diffs)
54 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XR_001780005.1 27.3% (many diffs)
55 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XR_001780006.1 26.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2379
ORF length:
2313
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga agaattgcat agcctggacc cacgacggca ggaattattg gaggccaggt
121ttactggagt aggtgttagt aagggaccac ttaatagtga gtcttccaac cagagcttgt
181gcagcgtcgg atccttgagt gataaagaag tagagactcc cgagaaaaag cagaatgacc
241agcgaaatcg gaaaagaaaa gctgaaccat atgaaactag ccaagggaaa ggcactccta
301ggggacataa aattagtgat tactttgagt ttgctggggg aagcgcgcca ggaaccagcc
361ctggcagaag tgttccacca gttgcacgat cctcaccgca acattcctta tccaatccct
421taccgcgacg agtagaacag cccctctatg gtttagatgg cagtgctgca aaggaggcaa
481cggaggagca gtctgctctg ccaaccctca tgtcagtgat gctagcaaaa cctcggcttg
541acacagagca gctggcgcaa aggggagctg gcctctgctt cacttttgtt tcagctcagc
601aaaacagtcc ctcatctacg ggatctggca acacagagca ttcctgcagc tcccaaaaac
661agatctccat ccagcacaga cagacccagt ccgacctcac aatagaaaaa atatctgcac
721tagaaaacag taagaattct gacttagaga agaaggaggg aagaatagat gatttattaa
781gagccaactg tgatttgaga cggcagattg atgaacagca aaagatgcta gagaaataca
841aggaacgatt aaatagatgt gtgacaatga gcaagaaact ccttatagaa aagtcaaaac
901aagagaagat ggcgtgtaga gataagagca tgcaagaccg cttgagactg ggccacttta
961ctactgtccg acacggagcc tcatttactg aacagtggac agatggttat gcttttcaga
1021atcttatcaa gcaacaggaa aggataaatt cacagaggga agagatagaa agacaacgga
1081aaatgttagc aaagcggaaa cctcctgcca tgggtcaggc ccctcctgca accaatgagc
1141agaaacagcg gaaaagcaag accaatggag ctgaaaatga aacgccctct tctgggaata
1201cagagctaaa ggatacagcc ccagccttag gagcccacag tttacttagg ttaacgttag
1261cagaatacca tgaacaagaa gaaatcttca aactcagatt aggtcatctt aaaaaggagg
1321aagcagagat ccaggcagag ctggagagac tagaaagggt tagaaatcta catatcaggg
1381aactaaaaag gatacataat gaagataatt cacaatttaa agatcatcca acgctaaatg
1441acagatattt gttgttacat cttttgggta gaggaggttt cagtgaagtt tacaaggcat
1501ttgatctaac agagcaaaga tacgtagctg tgaaaattca ccagttaaat aaaaactgga
1561gagatgagaa aaaggagaat taccacaagc atgcatgtag ggaataccgg attcataaag
1621agctggatca tcccagaata gttaagctgt atgattactt ttcactggat actgactcgt
1681tttgtacagt attagaatac tgtgagggaa atgatctgga cttctacctg aaacagcaca
1741aattaatgtc ggagaaagag gcccggtcca ttatcatgca gattgtgaat gctttaaagt
1801acttaaatga aataaaacct cccatcatac actatgacct caaaccaggt aatattcttt
1861tagtaaatgg tacagcgtgt ggagagataa aaattacaga ttttggtctt tcgaagatca
1921tggatgatga tagctacaat tcagtggatg gcatggagct aacatcacaa ggtgctggta
1981cttattggta tttaccacca gagtgttttg tggttgggaa agaaccacca aagatctcaa
2041ataaagttga tgtgtggtcg gtgggtgtga tcttctatca gtgtctttat ggaaggaagc
2101cttttggcca taaccagtct cagcaagaca tcctacaaga gaatacgatt cttaaagcta
2161ctgaagtgca gttcccgcca aagccagtag taacacctga agcaaaggcg tttattcgac
2221gatgcttggc ctaccgaaag gaggaccgca ttgatgtcca gcagctggcc tgtgatccct
2281acttgttgcc tcacatccga aagtcagtct ctacaagtag ccctgctgga gctgctattg
2341catcaacctc tggggcgtcc aataacagtt cttctaattg cccaactttc ttgtacaaag
2401tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
2461gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)