Construct: ORF ccsbBroad304_15055
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018958.1_s304c1, BRDN0000459442
- Derived from:
- ccsbBroadEn_15055
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MINK1 (50488)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15055
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_153827.5 | 99.5% | 18.4% | (many diffs) |
| 2 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_011523906.1 | 99.3% | 18.5% | (many diffs) |
| 3 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_001024937.4 | 98% | 18.7% | (many diffs) |
| 4 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_006721531.2 | 97.4% | 18% | (many diffs) |
| 5 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_006721532.2 | 97.3% | 18% | (many diffs) |
| 6 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024704.1 | 97.2% | 18.1% | (many diffs) |
| 7 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_015716.5 | 96.8% | 18.9% | (many diffs) |
| 8 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_170663.5 | 96.2% | 18.8% | (many diffs) |
| 9 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024705.1 | 95.9% | 18.3% | (many diffs) |
| 10 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_006721536.2 | 94.7% | 18.5% | (many diffs) |
| 11 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024706.1 | 94.6% | 18.5% | (many diffs) |
| 12 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024707.2 | 93.4% | 14% | (many diffs) |
| 13 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_001321236.2 | 92.6% | 14.7% | (many diffs) |
| 14 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024708.1 | 92.2% | 12.8% | (many diffs) |
| 15 | human | 50488 | MINK1 | misshapen like kinase 1 | XR_001752522.2 | 77.7% | (many diffs) | |
| 16 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_001045959.1 | 90.5% | 18.3% | (many diffs) |
| 17 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533681.3 | 90% | 18.2% | (many diffs) |
| 18 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_001045964.1 | 89.9% | 18.2% | (many diffs) |
| 19 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533679.2 | 88.6% | 17.9% | (many diffs) |
| 20 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533677.2 | 88.1% | 17.8% | (many diffs) |
| 21 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533678.2 | 88% | 17.8% | (many diffs) |
| 22 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_016713.2 | 87.9% | 18.8% | (many diffs) |
| 23 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_176893.2 | 87.4% | 18.7% | (many diffs) |
| 24 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_017314646.1 | 86% | 18.4% | (many diffs) |
| 25 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533682.3 | 85.5% | 18.3% | (many diffs) |
| 26 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_017314645.1 | 85.4% | 18.3% | (many diffs) |
| 27 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XR_388490.3 | 68.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 891
- ORF length:
- 822
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcgaccca gcccccgccc gcagcctgga cgacatcgac ctgtccgccc 121 tgcgggaccc tgctgggatc tttgagcttg tggaggtggt cggcaatgga acctacggac 181 aggtgtacaa gggtcggcat gtcaagacgg ggcagctggc tgccatcaag gtcatggatg 241 tcacggagga cgaggaggaa gagatcaaac aggagatcaa catgctgaaa aagtactctc 301 accaccgcaa catcgccacc tactacggag ccttcatcaa gaagagcccc ccgggaaacg 361 atgaccagct ctggctggtg atggagttct gtggtgctgg ttcagtgact gacctggtaa 421 agaacacaaa aggcaacgcc ctgaaggagg actgtatcgc ctatatctgc agggagatcc 481 tcaggggtct ggcccatctc catgcccaca aggtgatcca tcgagacatc aaggggcaga 541 atgtgctgct gacagagaat gctgaggtca agctagtgga ttttggggtg agtgctcagc 601 tggaccgcac cgtgggcaga cggaacactt tcattgggac tccctactgg atggctccag 661 aggtcatcgc ctgtgatgag aaccctgatg ccacctatga ttacaggagt gatatttggt 721 ctctaggaat cacagccatc gagatggcag agggagcccc ccctctgtgt gacatgcacc 781 catgcgagcc ctcttcctca ttcctcggaa ccctccgccc aggctcaagt cccaggagtg 841 gtctaagaag ttcattgact tcattgacac atgtctcatc aagacttacc tgagccgccc 901 acccacggag cagctactga agtttccctt catccgggac cagcccacgg agcggcaggt 961 ccgcatccag cttaaggacc acattgaccg atcccggaag aagcggggtg agaaagagga 1021 gacagaatat gagtacagcg gcagcgagga ggaagatgac agccatggag aggaaggaga 1081 gccaagctcc atcatgaacg tgcctggaga gtcgactcta cgccgggagt ttctccggct 1141 ccagcaggaa aataagagca actcagaggc tttaaaacag cagcagcagc tgcagcagca 1201 gcagcagcga gaccccgagg cacacatcaa acacctgctg caccagcggc agcggcgcat 1261 agaggagcag aaggaggagc ggcgccgcgt ggaggagcaa cagcggcggg agcgggagca 1321 gcggaagctg caggagaagg agcagcagcg gcggctggag gacatgcagg ctctgcggcg 1381 ggaggaggag cggcggcagg cggagcgcga gcaggaatac aagcggaagc agctggagga 1441 gcagcggcag tcagaacgtc tccagaggca gctgcagcag gagcatgcct acctcaagtc 1501 cctgcagcag cagcaacagc agcagcagct tcagaaacag cagcagcagc agctcctgcc 1561 tggggacagg aagcccctgt accattatgg tcggggcatg aatcccgctg acaaaccagc 1621 ctgggcccga gaggtagaag agagaacaag gatgaacaag cagcagaact ctcccttggc 1681 caagagcaag ccaggcagca cggggcctga gccccccatc ccccaggcct ccccagggcc 1741 cccaggaccc ctttcccaga ctcctcctat gcagaggccg gtggagcccc aggagggacc 1801 gcacaagagc ctggtggcac accgggtccc actgaagcca tatgcagcac ctgtaccccg 1861 atcccagtcc ctgcaggacc agcccacccg aaacctggct gccttcccag cctcccatga 1921 ccccgaccct gccatccccg cacccactgc cacgcccagt gcccgaggag ctgtcatccg 1981 ccagaattca gaccccacct ctgaaggacc tggccccagc ccgaatcccc cagcctgggt 2041 ccgcccagat aacgaggccc cacccaaggt gcctcagagg acctcatcta tcgccactgc 2101 ccttaacacc agtggggccg gagggtcccg gccagcccag gcagtccgtg ccagacctcg 2161 cagcaactcc gcctggcaaa tctatctgca aaggcgggca gagcggggca ccccaaagcc 2221 tccagggccc cctgctcagc cccctggccc gcccaacgcc tctagtaacc ccgacctcag 2281 gaggagcgac cctggctggg aacgctcgga cagcgtcctt ccagcctctc acgggcacct 2341 cccccaggct ggctcactgg agcggaaccg cgtgggagcc tcctccaaac tggacagctc 2401 ccctgtgctc tcccctggga ataaagccaa gcccgacgac caccgctcac ggccaggccg 2461 gcccgcagac tttgtgttgc tgaaagagcg gactctggac gaggcccctc ggcctcccaa 2521 gaaggccatg gactactcgt cgtccagcga ggaggtggaa agcagtgagg acgacgagga 2581 ggaaggcgaa ggcgggccag cagaggggag cagagatacc cctgggggcc gcagcgatgg 2641 ggatacagac agcgtcagca ccatggtggt ccacgacgtc gaggagatca ccgggaccca 2701 gcccccatac gggggcggca ccatggtggt ccagcgcacc cctgaagagg agcggaacct 2761 gctgcatgct gacagcaatg ggtacacaaa cctgcctgac gtggtccagc ccagccactc 2821 acccaccgag aacagcaaag gccaaagccc accctcgaag gatgggagtg gtgactacca 2881 gtctcgtggg ctggtaaagg cccctggcaa gagctcgttc acgatgtttg tggatctagg 2941 gatctaccag cctggaggca gtggggacag catccccatc acagccctag tgggtggaga 3001 gggcactcgg ctcgaccagc tgcagtacga cgtgaggaag ggttctgtgg tcaacgtgaa 3061 tcccaccaac acccgggccc acagtgagac ccctgagatc cggaagtaca agaagcgatt 3121 caactccgag atcctctgtg cagccctttg gggggtcaac ctgctggtgg gcacggagaa 3181 cgggctgatg ttgctggacc gaagtgggca gggcaaggtg tatggactca ttgggcggcg 3241 acgcttccag cagatggatg tgctggaggg gctcaacctg cttcatcacc attctcaggg 3301 aaaaaggaac aaactgcggg tgtattacct gtcctggctc cggaacagat tctgcacatg 3361 acccagagtg agagagcagg gctggaccac cgtggggaca tggagggctg cgggcactac 3421 cgtgttgtga aatacgagcg gattaagttc ctggtcatcg ccctcaagag ctccgtggag 3481 gtgtatgcct gggcccccaa accctaccac aaattcatgg ccttcaagtc ctttgccgac 3541 ctcccccacc gccctctgct ggtcgacctg acagtagagg aggggcagcg gctcaaggtc 3601 atctatggct ccagtgctgg cttccatgct gtggatgtcg actcggggaa cagctatgac 3661 atctacatcc ctgtgcacat ccagagccag atcacgcccc atgccatcat cttcctcccc 3721 aacaccgacg gcatggagat gctgctgtgc tacgaggacg agggtgtcta cgtcaacacg 3781 tacgggcgca tcattaagga tgtggtgctg cagtgggggg agatgcctac ttctgtggcc 3841 tacatctgct ccaaccagat aatgggctgg ggtgagaaag ccattgagat ccgctctgtg 3901 gagacgggcc acctcgacgg ggtcttcatg cacaaacgag ctcagaggct caagttcctg 3961 tgtgagcgga atgacaaggt gttttttgcc tcagtccgct ctgggggcag cagccaagtt 4021 tacttcatga ctctgaaccg taactgcatc atgaactggt tgccaacttt cttgtacaaa 4081 gtggttggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct 4141 agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggatta