Construct: ORF ccsbBroad304_15062

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000647.2_s304c1, BRDN0000459545
Derived from:
ccsbBroadEn_15062
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDKL3 (51265)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15062
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001113575.2 100% 100%
2 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009533.1 98% 98% 540_575del
3 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009534.1 95.6% 95.3% (many diffs)
4 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446090.1 95.6% 95.3% (many diffs)
5 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446091.1 95.6% 94.8% (many diffs)
6 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446092.1 94.7% 95% (many diffs)
7 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009532.1 94.7% 91.9% 1718_1788del;1821_1822ins26
8 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446088.1 94.7% 91.9% 1718_1788del;1821_1822ins26
9 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446089.1 94.7% 91.9% 1718_1788del;1821_1822ins26
10 human 51265 CDKL3 cyclin dependent kinase like 3 XM_011543429.2 92.9% 90.2% 540_575del;1754_1824del;1857_1858ins26
11 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446086.1 92.9% 90.2% 540_575del;1754_1824del;1857_1858ins26
12 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446087.1 92.9% 90.2% 540_575del;1754_1824del;1857_1858ins26
13 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009536.1 80% 77.5% (many diffs)
14 human 51265 CDKL3 cyclin dependent kinase like 3 NM_016508.3 76.8% 76.8% 1365_1366ins411
15 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446093.1 69.2% 65.3% (many diffs)
16 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446094.1 68.4% 62.4% (many diffs)
17 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446103.1 68% 68% 0_1ins567
18 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446095.1 67.2% 64.9% (many diffs)
19 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001300853.1 65.8% 65.4% (many diffs)
20 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001349363.1 64% 61.6% 0_1ins567;1151_1221del;1254_1255ins26
21 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446096.1 64% 61.6% 0_1ins567;1151_1221del;1254_1255ins26
22 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446097.1 64% 61.6% 0_1ins567;1151_1221del;1254_1255ins26
23 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446098.1 64% 61.6% 0_1ins567;1151_1221del;1254_1255ins26
24 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446099.1 64% 61.6% 0_1ins567;1151_1221del;1254_1255ins26
25 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446100.1 64% 61.6% 0_1ins567;1151_1221del;1254_1255ins26
26 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446101.1 64% 61.6% 0_1ins567;1151_1221del;1254_1255ins26
27 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446102.1 64% 61.6% 0_1ins567;1151_1221del;1254_1255ins26
28 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446104.1 64% 61.6% 0_1ins567;1151_1221del;1254_1255ins26
29 human 51265 CDKL3 cyclin dependent kinase like 3 XR_002956161.1 61.3% (many diffs)
30 human 51265 CDKL3 cyclin dependent kinase like 3 XR_002956159.1 60.9% (many diffs)
31 human 51265 CDKL3 cyclin dependent kinase like 3 XR_002956160.1 57.8% (many diffs)
32 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001349364.1 46.8% 44.7% 0_1ins885;833_903del;936_937ins26
33 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001349365.1 46.8% 44.7% 0_1ins885;833_903del;936_937ins26
34 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001349366.1 46.8% 44.7% 0_1ins885;833_903del;936_937ins26
35 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009544.2 46.8% 44.7% 0_1ins885;833_903del;936_937ins26
36 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009545.2 46.8% 44.7% 0_1ins885;833_903del;936_937ins26
37 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009546.1 46.8% 44.7% 0_1ins885;833_903del;936_937ins26
38 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009547.1 46.8% 44.7% 0_1ins885;833_903del;936_937ins26
39 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446105.1 46.8% 44.7% 0_1ins885;833_903del;936_937ins26
40 mouse 213084 Cdkl3 cyclin-dependent kinase-like 3 NM_001166657.1 50.9% 47.2% (many diffs)
41 mouse 213084 Cdkl3 cyclin-dependent kinase-like 3 XM_017314422.1 50.9% 47.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1842
ORF length:
1776
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gatgtatgaa acccttggaa aagtgggaga gggaagttac ggaacagtca
121tgaaatgtaa acataagaat actgggcaga tagtggccat taagatattt tatgagagac
181cagaacaatc tgtcaacaaa attgcgatga gagaaataaa gtttctaaag caatttcatc
241acgaaaacct ggtcaatctg attgaagttt ttagacagaa aaagaaaatt catttggtat
301ttgaatttat tgaccacaca gtattagatg agttacaaca ttattgtcat ggactagaga
361gtaagcgact tagaaaatac ctcttccaga tccttcgagc aattgactat cttcacagta
421ataatatcat tcatcgagat ataaaacctg agaatatttt agtatcccag tcaggaatta
481ctaagctctg tgattttggt tttgcacgaa cactagcagc tcctggggac atttatacgg
541actatgtggc cacacgctgg tatagagctc ccgaattagt attaaaagat acttcttatg
601gaaaacctgt ggatatctgg gctttgggct gtatgatcat tgagatggcc actggaaatc
661cctatcttcc tagtagttct gatttggatt tactccataa aattgttttg aaagtgggca
721atttgtcacc tcacttgcag aatatctttt ccaagagccc catttttgct ggggtagttc
781ttcctcaagt tcaacacccc aaaaatgcaa gaaaaaaata tccaaagctt aatggattgt
841tggcagatat agttcatgct tgtttacaaa ttgatcctgc tgacaggata tcatctagtg
901atcttttgca tcatgagtat tttactagag atggatttat tgaaaaattc atgccagaac
961tgaaagctaa attactgcag gaagcaaaag tcaattcatt aataaagcca aaagagagtt
1021ctaaagaaaa tgaactcagg aaagatgaaa gaaaaacagt ttataccaat acactgctaa
1081gtagttcagt tttgggaaag gaaatagaaa aagagaaaaa gcccaaggag atcaaagtca
1141gagttattaa agtcaaagga ggaagaggag atatctcaga accaaaaaag aaagagtatg
1201aaggtggact tggtcaacag gatgcaaatg aaaatgttca tcctatgtct ccagatacaa
1261aacttgtaac cattgaacca ccaaacccta tcaatcccag cactaactgt aatggcttga
1321aagaaaatcc acattgcgga ggttctgtga caatgccacc catcaatcta actaacagta
1381atttgatggc tgcaaatctc agttcaaatc tctttcaccc cagtgtgagg ttaactgaaa
1441gagcaaaaaa gagacgcact tcttcacaat ctattggaca agttatgcct aatagcaggc
1501aagaggatcc aggtcctatt caaagccaaa tggagaaggg tatatttaat gagcgaacag
1561gtcacagtga ccaaatggca aatgagaaca aaaggaagct gaatttttcc agatctgaca
1621ggaaagaatt ccattttcca gaattgcctg tcacaataca gtcaaaagat acaaaaggaa
1681tggaagttaa acagataaaa atgctgaaga gggagtcaaa gaaaacagag tcatctaaga
1741taccaacttt acttaacgtg gatcaaaatc aagaaaaaca agagggtgga gatggccatt
1801gcgaggggaa gaatttgaag agaaacaggt tttttttctg gtacccaact ttcttgtaca
1861aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1921ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)