Transcript: Human XM_024446105.1

PREDICTED: Homo sapiens cyclin dependent kinase like 3 (CDKL3), transcript variant X31, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDKL3 (51265)
Length:
1792
CDS:
601..1539

Additional Resources:

NCBI RefSeq record:
XM_024446105.1
NBCI Gene record:
CDKL3 (51265)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024446105.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000367593 CCACCCATCAATCTAACTAAC pLKO_005 1006 CDS 100% 10.800 15.120 N CDKL3 n/a
2 TRCN0000002379 ACTAACTGTAATGGCTTGAAA pLKO.1 952 CDS 100% 5.625 7.875 N CDKL3 n/a
3 TRCN0000002380 CCATCAATCTAACTAACAGTA pLKO.1 1010 CDS 100% 4.950 6.930 N CDKL3 n/a
4 TRCN0000196738 GAGATCAAAGTCAGAGTTATT pLKO.1 778 CDS 100% 13.200 9.240 N CDKL3 n/a
5 TRCN0000196777 GCTGCAAATCTCAGTTCAAAT pLKO.1 1039 CDS 100% 13.200 9.240 N CDKL3 n/a
6 TRCN0000002377 GCCACCCATCAATCTAACTAA pLKO.1 1005 CDS 100% 5.625 3.938 N CDKL3 n/a
7 TRCN0000194700 CAGGATATCATCTAGTGATCT pLKO.1 534 5UTR 100% 4.950 3.465 N CDKL3 n/a
8 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 20 5UTR 100% 5.625 2.813 Y KLHL30 n/a
9 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 20 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024446105.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03262 pDONR223 100% 46.8% 44.7% None 0_1ins885;833_903del;936_937ins26 n/a
2 ccsbBroad304_03262 pLX_304 0% 46.8% 44.7% V5 0_1ins885;833_903del;936_937ins26 n/a
3 ccsbBroadEn_15062 pDONR223 0% 46.8% 44.7% None 0_1ins885;833_903del;936_937ins26 n/a
4 ccsbBroad304_15062 pLX_304 0% 46.8% 44.7% V5 0_1ins885;833_903del;936_937ins26 n/a
5 TRCN0000473541 CGACATTCATACACCTACACCTGC pLX_317 21.4% 46.8% 44.7% V5 0_1ins885;833_903del;936_937ins26 n/a
6 TRCN0000489746 AGCGACCGACGTAAGGTACTCGAG pLX_317 27.6% 26.3% 26.3% V5 0_1ins885;481_936delinsG n/a
7 TRCN0000489363 GTTGGTTGTTCTCCAAGACAACGT pLX_317 30.3% 26.3% 26.3% V5 (not translated due to prior stop codon) 0_1ins885;481_936del n/a
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