Transcript: Mouse XM_017314422.1

PREDICTED: Mus musculus cyclin-dependent kinase-like 3 (Cdkl3), transcript variant X40, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Cdkl3 (213084)
Length:
1803
CDS:
742..1803

Additional Resources:

NCBI RefSeq record:
XM_017314422.1
NBCI Gene record:
Cdkl3 (213084)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017314422.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000023138 CCTTCCTAGCAGTTCCGATTT pLKO.1 1341 CDS 100% 10.800 15.120 N Cdkl3 n/a
2 TRCN0000322368 CCTTCCTAGCAGTTCCGATTT pLKO_005 1341 CDS 100% 10.800 15.120 N Cdkl3 n/a
3 TRCN0000023136 CCGCACCTGCACAATATCTTT pLKO.1 1405 CDS 100% 5.625 7.875 N Cdkl3 n/a
4 TRCN0000023137 GCAACGAGAGAAATAAAGTTT pLKO.1 880 CDS 100% 5.625 7.875 N Cdkl3 n/a
5 TRCN0000322367 GCAACGAGAGAAATAAAGTTT pLKO_005 880 CDS 100% 5.625 7.875 N Cdkl3 n/a
6 TRCN0000023134 CGCCATTGAGTACCTGCATAA pLKO.1 1074 CDS 100% 10.800 7.560 N Cdkl3 n/a
7 TRCN0000195070 CTTTGGGCTGTATGATCATTG pLKO.1 1298 CDS 100% 10.800 7.560 N CDKL3 n/a
8 TRCN0000023135 CGGTCATGAAATGCAAACATA pLKO.1 791 CDS 100% 5.625 3.938 N Cdkl3 n/a
9 TRCN0000322366 CGGTCATGAAATGCAAACATA pLKO_005 791 CDS 100% 5.625 3.938 N Cdkl3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017314422.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489363 GTTGGTTGTTCTCCAAGACAACGT pLX_317 30.3% 66.1% 61% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000489746 AGCGACCGACGTAAGGTACTCGAG pLX_317 27.6% 66% 60.9% V5 (many diffs) n/a
3 ccsbBroadEn_03262 pDONR223 100% 50.9% 47.2% None (many diffs) n/a
4 ccsbBroad304_03262 pLX_304 0% 50.9% 47.2% V5 (many diffs) n/a
5 ccsbBroadEn_15062 pDONR223 0% 50.9% 47.2% None (many diffs) n/a
6 ccsbBroad304_15062 pLX_304 0% 50.9% 47.2% V5 (many diffs) n/a
7 TRCN0000473541 CGACATTCATACACCTACACCTGC pLX_317 21.4% 50.9% 47.2% V5 (many diffs) n/a
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