Transcript: Human XM_024446101.1

PREDICTED: Homo sapiens cyclin dependent kinase like 3 (CDKL3), transcript variant X24, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDKL3 (51265)
Length:
1922
CDS:
413..1669

Additional Resources:

NCBI RefSeq record:
XM_024446101.1
NBCI Gene record:
CDKL3 (51265)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146569 GAACTACCCCAGCAAAAATG pXPR_003 GGG 131 10% 3 0.4505 CDKL3 CDKL3 75605
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024446101.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000367593 CCACCCATCAATCTAACTAAC pLKO_005 1136 CDS 100% 10.800 15.120 N CDKL3 n/a
2 TRCN0000002379 ACTAACTGTAATGGCTTGAAA pLKO.1 1082 CDS 100% 5.625 7.875 N CDKL3 n/a
3 TRCN0000002380 CCATCAATCTAACTAACAGTA pLKO.1 1140 CDS 100% 4.950 6.930 N CDKL3 n/a
4 TRCN0000196738 GAGATCAAAGTCAGAGTTATT pLKO.1 908 CDS 100% 13.200 9.240 N CDKL3 n/a
5 TRCN0000314925 GATTTGGATTTACTCCATAAA pLKO_005 461 CDS 100% 13.200 9.240 N CDKL3 n/a
6 TRCN0000196777 GCTGCAAATCTCAGTTCAAAT pLKO.1 1169 CDS 100% 13.200 9.240 N CDKL3 n/a
7 TRCN0000195070 CTTTGGGCTGTATGATCATTG pLKO.1 402 5UTR 100% 10.800 7.560 N CDKL3 n/a
8 TRCN0000002377 GCCACCCATCAATCTAACTAA pLKO.1 1135 CDS 100% 5.625 3.938 N CDKL3 n/a
9 TRCN0000194700 CAGGATATCATCTAGTGATCT pLKO.1 664 CDS 100% 4.950 3.465 N CDKL3 n/a
10 TRCN0000314927 ATGGATTGTTGGCAGATATAG pLKO_005 612 CDS 100% 13.200 7.920 N CDKL3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024446101.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03262 pDONR223 100% 64% 61.6% None 0_1ins567;1151_1221del;1254_1255ins26 n/a
2 ccsbBroad304_03262 pLX_304 0% 64% 61.6% V5 0_1ins567;1151_1221del;1254_1255ins26 n/a
3 ccsbBroadEn_15062 pDONR223 0% 64% 61.6% None 0_1ins567;1151_1221del;1254_1255ins26 n/a
4 ccsbBroad304_15062 pLX_304 0% 64% 61.6% V5 0_1ins567;1151_1221del;1254_1255ins26 n/a
5 TRCN0000473541 CGACATTCATACACCTACACCTGC pLX_317 21.4% 64% 61.6% V5 0_1ins567;1151_1221del;1254_1255ins26 n/a
6 TRCN0000489746 AGCGACCGACGTAAGGTACTCGAG pLX_317 27.6% 43.8% 43.8% V5 0_1ins567;799_1254delinsG n/a
7 TRCN0000489363 GTTGGTTGTTCTCCAAGACAACGT pLX_317 30.3% 43.8% 43.8% V5 (not translated due to prior stop codon) 0_1ins567;799_1254del n/a
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