Construct: ORF ccsbBroad304_15314

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017882.2_s304c1, BRDN0000459580
Derived from:
ccsbBroadEn_15314
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PI4KAP1 (728233)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15314
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 375133 PI4KAP2 phosphatidylinositol 4-kina... NR_003700.1 34.3% (many diffs)
2 human 728233 PI4KAP1 phosphatidylinositol 4-kina... NR_003563.1 33.3% 1_30del;817_2360del
3 human 5297 PI4KA phosphatidylinositol 4-kina... XM_017028830.1 17.4% 17.2% (many diffs)
4 human 5297 PI4KA phosphatidylinositol 4-kina... XM_005261635.1 13.8% 13.7% (many diffs)
5 human 5297 PI4KA phosphatidylinositol 4-kina... NM_001362862.1 12.3% 12.2% (many diffs)
6 human 5297 PI4KA phosphatidylinositol 4-kina... NM_001362863.1 12.3% 12.1% (many diffs)
7 human 5297 PI4KA phosphatidylinositol 4-kina... NM_058004.4 12.2% 12% (many diffs)
8 human 5297 PI4KA phosphatidylinositol 4-kina... XR_937868.1 11.6% (many diffs)
9 human 5297 PI4KA phosphatidylinositol 4-kina... XM_011530226.1 8.4% 8.4% (many diffs)
10 mouse 224020 Pi4ka phosphatidylinositol 4-kina... XM_006522037.2 19.7% 21.3% (many diffs)
11 mouse 224020 Pi4ka phosphatidylinositol 4-kina... NM_001001983.2 11.3% 12.2% (many diffs)
12 mouse 224020 Pi4ka phosphatidylinositol 4-kina... XM_006522034.1 10.9% 11.8% (many diffs)
13 mouse 224020 Pi4ka phosphatidylinositol 4-kina... XM_006522036.3 7.7% 8.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
852
ORF length:
786
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac agtggagcag aaatttggcc tgttttctgc tgagataaag gaagcagacc
121ccctggctgc ctcggaagca agtcaaccca aaccctgtcc ccccgaagtg accccccact
181acatctggat cgacttcctg gtgcagcggt ttgagatcgc caagtactgc agctctgacc
241aagtggagat cttctccagc ctgctgcagc gctccatgtc cctgaacatc ggtggggcca
301aggggagcat gaaccggcat gtggcggcca tcgggccccg cttcaagctg ctgaccctgg
361ggctgtccct cctgcatgcc gatgtggttc caaatgcaac catccgcaat gtgcttcgcg
421agaagatcta ctccactgcc tttgactact tcagctgtcc cccaaagttt cctactcaag
481gagagaagcg gctgcgtgaa gacataagca tcatgattaa attttggacc gccatgttct
541cagataagaa gtacctgacc gccagccagc ttgttccccc agctgacatc ggcgacctcc
601gggagcagtt agtagaggag aacacaggct ccttgtcggg cccagcgaag gacttttacc
661agcgggagtt tgatttcttt aacaagatca ccaacgtgtc ggctgtcatc aagccctacc
721ctaaaggcga ccagagaaag aaggcttgtc tgtcggccct gtctgaagtg acggtgcagc
781caggctgctc cctgcccagc aaccctgaag ccattgtgct ggacgtcgac tacaagtctg
841ggaccccgat gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
901tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
961caatcaacct ctggatta

Download FASTA (ORF) (Full)