Transcript: Human XM_011530226.1

PREDICTED: Homo sapiens phosphatidylinositol 4-kinase alpha (PI4KA), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PI4KA (5297)
Length:
4269
CDS:
4..4254

Additional Resources:

NCBI RefSeq record:
XM_011530226.1
NBCI Gene record:
PI4KA (5297)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145268 ATAGTCTGTTATTACCTGTG pXPR_003 TGG 1673 39% 13 1.266 PI4KA PI4KA 76816
2 BRDN0001147752 GTAGGTGAAGAAGATCGTTG pXPR_003 AGG 1102 26% 9 0.6051 PI4KA PI4KA 76819
3 BRDN0001145914 GGTCCAGTTACCTATAGCCG pXPR_003 TGG 2186 51% 17 0.364 PI4KA PI4KA 76818
4 BRDN0001145538 CTGGCCAGAAGAATGGTACG pXPR_003 AGG 2542 60% 21 0.0988 PI4KA PI4KA 76817
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011530226.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199319 CGGAAGCAAGTCAACCCAAAC pLKO.1 3926 CDS 100% 6.000 3.000 Y PI4KAP2 n/a
2 TRCN0000195645 CTGACCAAGTGGAGATCTTCT pLKO.1 4028 CDS 100% 4.950 2.475 Y PI4KAP2 n/a
3 TRCN0000052625 CCACTACATCTGGATCGACTT pLKO.1 3969 CDS 100% 4.050 2.025 Y PI4KAP2 n/a
4 TRCN0000052627 CCGATGTGGTTCCAAATGCAA pLKO.1 4172 CDS 100% 3.000 1.500 Y PI4KAP2 n/a
5 TRCN0000174244 CCGATGTGGTTCCAAATGCAA pLKO.1 4172 CDS 100% 3.000 1.500 Y PI4KAP2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011530226.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488521 GAACTTAAACATACCTTACTTGCC pLX_317 6.4% 62.4% 62.3% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000488590 GTCGTTCACTGAGACAACATCATC pLX_317 5.7% 62.4% 62.3% V5 (many diffs) n/a
3 ccsbBroadEn_10407 pDONR223 100% 8.4% 8.4% None (many diffs) n/a
4 ccsbBroad304_10407 pLX_304 0% 8.4% 8.4% V5 (many diffs) n/a
5 TRCN0000468809 TGGCAGTTTGCGAAACGGTAAAAA pLX_317 50.2% 8.4% 8.4% V5 (many diffs) n/a
6 ccsbBroadEn_15314 pDONR223 0% 8.4% 8.4% None (many diffs) n/a
7 ccsbBroad304_15314 pLX_304 0% 8.4% 8.4% V5 (many diffs) n/a
8 TRCN0000466007 ACAACCTTGACCTCCTCTTCCTCT pLX_317 48.3% 8.4% 8.4% V5 (many diffs) n/a
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