Construct: ORF ccsbBroad304_15448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013831.1_s304c1, BRDN0000402506
- Derived from:
- ccsbBroadEn_15448
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HNMT (3176)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15448
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3176 | HNMT | histamine N-methyltransferase | NM_001024074.3 | 100% | 100% | |
| 2 | human | 3176 | HNMT | histamine N-methyltransferase | NM_001024075.2 | 38.2% | 36.5% | (many diffs) |
| 3 | human | 3176 | HNMT | histamine N-methyltransferase | XM_017003949.2 | 29.9% | 28.5% | (many diffs) |
| 4 | human | 3176 | HNMT | histamine N-methyltransferase | NM_006895.3 | 16.5% | 15.7% | (many diffs) |
| 5 | human | 3176 | HNMT | histamine N-methyltransferase | XR_002959286.1 | 4.3% | 1_97del;251_3490del | |
| 6 | mouse | 140483 | Hnmt | histamine N-methyltransferase | NM_080462.2 | 15.1% | 13.8% | (many diffs) |
| 7 | mouse | 140483 | Hnmt | histamine N-methyltransferase | XM_006497671.3 | 15.1% | 13.8% | (many diffs) |
| 8 | mouse | 140483 | Hnmt | histamine N-methyltransferase | XM_006497670.3 | 14.5% | 13.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 219
- ORF length:
- 153
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc atcttccatg aggagcttgt tttctgacca cgggaaatat gttgaatctt 121 tccggaggtt tctcaaccat tccacggaac accagtgcat gcaggaattc atggacaaga 181 agctgccagg cataatagga agataccaga attgctgttn nccaactttc ttgtacaaag 241 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 301 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta