Construct: ORF ccsbBroad304_15539
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001605.1_s304c1, BRDN0000401959
- Derived from:
- ccsbBroadEn_15539
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PKN2 (5586)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15539
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5586 | PKN2 | protein kinase N2 | NM_001320707.1 | 99.8% | 99.8% | 421T>C;1533T>C;2239T>C |
| 2 | human | 5586 | PKN2 | protein kinase N2 | XM_017001783.2 | 98.1% | 98.1% | (many diffs) |
| 3 | human | 5586 | PKN2 | protein kinase N2 | NM_006256.4 | 95% | 95% | (many diffs) |
| 4 | human | 5586 | PKN2 | protein kinase N2 | NM_001320709.2 | 93.3% | 93.3% | (many diffs) |
| 5 | human | 5586 | PKN2 | protein kinase N2 | XM_017001782.2 | 88.7% | 88.6% | (many diffs) |
| 6 | human | 5586 | PKN2 | protein kinase N2 | NM_001320708.2 | 79% | 79% | (many diffs) |
| 7 | human | 5586 | PKN2 | protein kinase N2 | XM_011541772.2 | 61.8% | 61.5% | (many diffs) |
| 8 | mouse | 109333 | Pkn2 | protein kinase N2 | NM_178654.4 | 85.5% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2877
- ORF length:
- 2808
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtccaac cccgaacggg gggagattct gctcacggaa ctgcaggggg 121 attcccgaag tcttccgttt tctgagaatg tgagtgctgt tcaaaaatta gacttttcag 181 atacaatggt gcagcagaaa ttggatgata tcaaggatcg aattaagaga gaaataagga 241 aagaactgaa aatcaaagaa ggagctgaaa atctgaggaa agtcacaaca gataaaaaaa 301 gtttggctta tgtagacaac attttgaaaa aatcaaataa aaaattagaa gaactacatc 361 acaagctgca ggaattaaat gcacatattg ttgtatcaga tccagaagat attacagatt 421 gcccaaggac tccagatact ccaaataatg accctcgttg ttctactagc aacaatagat 481 tgaaggccct acaaaaacaa ttggatatag aacttaaagt aaaacaaggt gcagagaata 541 tgatacagat gtattcaaat ggatcttcaa aggatcggaa actccatggt acagctcagc 601 aactgctcca ggacagcaag acaaaaatag aagtcatacg aatgcagatt cttcaggcag 661 tccagactaa tgaattggct tttgataatg caaaacctgt gataagtcct cttgaacttc 721 ggatggaaga attaaggcat cattttagga tagagtttgc agtagcagaa ggtgcaaaga 781 atgtaatgaa attacttggc tcaggaaaag taacagacag aaaagcactt tcagaagctc 841 aagcaagatt taatgaatca agtcagaagt tggacctttt aaagtattca ttagagcaaa 901 gattaaacga agtccccaag aatcatccca aaagcaggat tattattgaa gaactttcac 961 ttgttgctgc atcaccaaca ctaagtccac gtcaaagtat gatatctacg caaaatcaat 1021 atagtacact atccaaacca gcagcactaa caggtacttt ggaagttcgt cttatgggct 1081 gccaagatat cctagagaat gtccctggac ggtcaaaagc aacatcagtt gcactgcctg 1141 gttggagtcc aagtgaaacc agatcatctt tcatgagcag aacgagtaaa agtaaaagcg 1201 gaagtagtcg aaatcttcta aaaaccgatg acttgtccaa tgatgtctgt gctgttttga 1261 agctcgataa tactgtggtt ggccaaacta gctggaaacc catttccaat cagtcatggg 1321 accagaagtt tacactggaa ctggacaggg ttaccttttt taatccagtt attgaaagaa 1381 gaccaaaact tcaaagacaa aagaaaattt tttcaaagca acaaggcaaa acatttctca 1441 gagctcctca aatgaatatt aatattgcca cttggggaag gctagtaaga agagctattc 1501 ctacagtaaa tcattctggc accttcagcc ctcaagctcc tgtgcctact acagtgccag 1561 tggttgatgt acgcatccct caactagcac ctccagctag cgattctaca gtaaccaaat 1621 tggactttga tcttgagcct gaacctcctc cagccccacc acgagcttct tctcttggag 1681 aaatagatga atcttctgaa ttaagagttt tggatatacc aggacaggat tcagagactg 1741 tttttgatat tcagaatgac agaaatagta tacttccaaa atctcaatct gaatacaagc 1801 ctgatactcc tcagtcaggc ctagaatata gtggtattca agaacttgag gacagaagat 1861 ctcagcaaag gtttcagttt aatctacaag atttcaggtg ttgtgctgtc ttgggaagag 1921 gacattttgg aaaggtgctt ttagctgaat ataaaaacac aaatgagatg tttgctataa 1981 aagccttaaa gaaaggagat attgtggctc gagatgaagt agacagcctg atgtgtgaaa 2041 aaagaatttt tgaaactgtg aatagtgtaa ggcatccctt tttggtgaac ctttttgcat 2101 gtttccaaac caaagagcat gtttgctttg taatggaata tgctgccggt ggggacctaa 2161 tgatgcacat tcatactgat gtcttttctg aaccaagagc tgtattttat gctgcttgtg 2221 tagttcttgg gttgcagtat ttacatgaac acaaaattgt ttatagagat ttgaaattgg 2281 ataacttatt gctagataca gagggccttg tgaaaattgc tgattttggt ctttgcaaag 2341 aaggaatggg atatggagat agaacaagca cattttgtgg cactcctgaa tttcttgccc 2401 cagaagtatt aacagaaact tcttatacaa gggctgtaga ttggtggggc cttggcgtgc 2461 ttatatatga aatgcttgtt ggtgagtctc cctttcctgg tgatgatgaa gaggaagttt 2521 ttgacagtat tgtaaatgat gaagtaaggt atccaaggtt cttatctaca gaagccattt 2581 ctataatgag aaggctgtta agaagaaatc ctgaacggcg ccttggggct agcgagaaag 2641 atgcagagga tgtaaaaaag cacccatttt tccggctaat tgattggagc gctctgatgg 2701 acaaaaaagt aaagccacca tttataccta ccataagagg acgagaagat gttagtaatt 2761 ttgatgatga atttacctca gaagcaccta ttctgactcc acctcgagaa ccaaggatac 2821 tttcggaaga ggagcaggaa atgttcagag attttgacta cattgctgat tggtgttnnc 2881 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2941 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 3001 tta