Construct: ORF ccsbBroad304_15560
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008309.1_s304c1, BRDN0000407120
- Derived from:
- ccsbBroadEn_15560
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAP1GDS1 (5910)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15560
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_021159.5 | 99.9% | 99.8% | 170G>C |
2 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100426.2 | 99.7% | 99.6% | 170G>C;1302_1304delAGC |
3 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454164.1 | 99.7% | 99.5% | 3_4insGCA;167G>C |
4 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100427.2 | 99.6% | 99.3% | 3_4insGCA;167G>C;1299_1301delAGC |
5 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454165.1 | 91.8% | 91.7% | 170G>C;364_365ins147 |
6 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100428.2 | 91.7% | 91.6% | 170G>C;364_365ins147;1155_1157delAGC |
7 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454166.1 | 91.7% | 91.4% | 3_4insGCA;167G>C;361_362ins147 |
8 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100429.2 | 91.5% | 91.2% | (many diffs) |
9 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454167.1 | 86.5% | 86.5% | 0_1ins243;1059_1061delAGC |
10 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454168.1 | 86.5% | 86.5% | 0_1ins243;1059_1061delAGC |
11 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100430.2 | 84.6% | 84.3% | 170G>C;364_365ins276;1026_1028delAGC |
12 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454172.1 | 78.5% | 78.5% | 0_1ins243;121_122ins147 |
13 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454173.1 | 78.5% | 78.5% | 0_1ins243;121_122ins147 |
14 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454169.1 | 78.4% | 78.4% | 0_1ins243;121_122ins147;912_914delAGC |
15 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454170.1 | 78.4% | 78.4% | 0_1ins243;121_122ins147;912_914delAGC |
16 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454171.1 | 78.4% | 78.4% | 0_1ins243;121_122ins147;912_914delAGC |
17 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454174.1 | 70.3% | 70.3% | 0_1ins537;765_767delAGC |
18 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454175.1 | 70.3% | 70.3% | 0_1ins537;765_767delAGC |
19 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454176.1 | 70.3% | 70.3% | 0_1ins537;765_767delAGC |
20 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XR_002959742.1 | 67.5% | (many diffs) | |
21 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XR_002959743.1 | 61.9% | (many diffs) | |
22 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XR_002959744.1 | 61.8% | (many diffs) | |
23 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | NM_001286759.1 | 89% | 95% | (many diffs) |
24 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | NM_001040690.2 | 88.9% | 94.9% | (many diffs) |
25 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | XM_017319566.1 | 81.3% | 87.6% | (many diffs) |
26 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | NM_145544.3 | 81.1% | 87.5% | (many diffs) |
27 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | XM_006501428.3 | 62.1% | 68% | (many diffs) |
28 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | XM_006501429.3 | 62.1% | 68% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1887
- ORF length:
- 1821
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agataatctc agtgatacct tgaagaagct gaagataaca gctgttgaca 121 agactgagga tagtttagaa ggatgcttgg attgtctgct tcaagccctg gctcaaaata 181 atacggaaac aagtgaaaaa atccaagcaa gtggaatact tcagctgttt gcaactctgt 241 tgactccaca gtcttcctgc aaagccaaag tagctaacat catagcagaa gtagccaaaa 301 atgagtttat gcgaattcca tgtgtggatg ctggattgat ttcaccactg gtgcagctgc 361 taaatagcaa agaccaggaa gtgctgcttc aaacgggcag ggctctagga aacatatgtt 421 acgatagcca tgagggcaga agtgcagttg accaagcagg tggtgcacag attgtaattg 481 accatttaag gtcactgtgc agtataacag atcccgccaa tgagaagctc ttgactgtct 541 tttgtggcat gctgatgaac tatagcaatg agaatgattc gcttcaagct cagcttatca 601 atatgggtgt tattcctacc ttagtgaaat tactgggcat ccactgccaa aatgcagctc 661 ttacagaaat gtgtcttgtt gcatttggta atttagcaga acttgagtca agtaaagaac 721 agtttgccag tacaaacatt gctgaagagc tagtaaaact cttcaagaaa caaatagaac 781 atgataagag agaaatgatt tttgaagttc ttgctccatt ggcagaaaat gatgctatta 841 aactacagct ggttgaagca ggcctagtag agtgtctact agagattgtt cagcaaaaag 901 tggatagtga caaagaagat gatattactg agctcaaaac tggttcagat ctcatggttt 961 tattacttct tggagatgaa tccatgcaga agttatttga aggaggaaaa ggtagtgtat 1021 ttcaaagggt actctcttgg atcccatcaa ataaccacca gctacagctt gctggagcat 1081 tggcaattgc aaattttgcc agaaatgatg caaattgtat tcatatggta gacaatggga 1141 ttgtagaaaa acttatggat ttactggaca gacatgtaga agatggaaat gtaacagtac 1201 agcatgcagc actaagtgcc ctcagaaacc tggccattcc agttataaat aaagcaaaga 1261 tgttatcagc tggggtcaca gaggcagttt tgaaatttct taaatctgaa atgcctcctg 1321 ttcagttcaa acttctggga acattaagaa tgttaataga tgcacaagaa gctgctgaac 1381 aattgggaaa gaatgttaag ttagtggagc gtttggtgga atggtgtgaa gccaaagatc 1441 atgctggtgt gatgggggag tcaaacagac tgctgtctgc ccttatacga cacagtaaat 1501 caaaagatgt aattaaaacc attgtgcaga gtggtggcat caagcatcta gttaccatgg 1561 caactagtga acatgtaata atgcagaatg aagctcttgt tgctttggca ttaatagcag 1621 ctttagaatt gggcactgct gagaaagatc tagaaagtgc taaacttgta cagattttac 1681 atagactgct agcagatgag agaagtgctc ctgaaatcaa atataattcc atggtcctga 1741 tatgtgctct tatgggatct gaatgtctac acaaggaagt acaggatttg gcttttctag 1801 atgtcgtatc caaacttcgc agtcatgaga acaaaagtgt tgcccagcag gcctctctca 1861 cagagcagag acttactgtg gaaagctnnc caactttctt gtacaaagtg gttggtaagc 1921 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1981 ggcgcgttaa gtcgacaatc aacctctgga tta