Construct: ORF ccsbBroad304_15560

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008309.1_s304c1, BRDN0000407120
Derived from:
ccsbBroadEn_15560
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RAP1GDS1 (5910)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15560
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_021159.5 99.9% 99.8% 170G>C
2 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100426.2 99.7% 99.6% 170G>C;1302_1304delAGC
3 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454164.1 99.7% 99.5% 3_4insGCA;167G>C
4 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100427.2 99.6% 99.3% 3_4insGCA;167G>C;1299_1301delAGC
5 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454165.1 91.8% 91.7% 170G>C;364_365ins147
6 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100428.2 91.7% 91.6% 170G>C;364_365ins147;1155_1157delAGC
7 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454166.1 91.7% 91.4% 3_4insGCA;167G>C;361_362ins147
8 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100429.2 91.5% 91.2% (many diffs)
9 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454167.1 86.5% 86.5% 0_1ins243;1059_1061delAGC
10 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454168.1 86.5% 86.5% 0_1ins243;1059_1061delAGC
11 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100430.2 84.6% 84.3% 170G>C;364_365ins276;1026_1028delAGC
12 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454172.1 78.5% 78.5% 0_1ins243;121_122ins147
13 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454173.1 78.5% 78.5% 0_1ins243;121_122ins147
14 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454169.1 78.4% 78.4% 0_1ins243;121_122ins147;912_914delAGC
15 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454170.1 78.4% 78.4% 0_1ins243;121_122ins147;912_914delAGC
16 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454171.1 78.4% 78.4% 0_1ins243;121_122ins147;912_914delAGC
17 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454174.1 70.3% 70.3% 0_1ins537;765_767delAGC
18 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454175.1 70.3% 70.3% 0_1ins537;765_767delAGC
19 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454176.1 70.3% 70.3% 0_1ins537;765_767delAGC
20 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XR_002959742.1 67.5% (many diffs)
21 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XR_002959743.1 61.9% (many diffs)
22 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XR_002959744.1 61.8% (many diffs)
23 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... NM_001286759.1 89% 95% (many diffs)
24 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... NM_001040690.2 88.9% 94.9% (many diffs)
25 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... XM_017319566.1 81.3% 87.6% (many diffs)
26 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... NM_145544.3 81.1% 87.5% (many diffs)
27 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... XM_006501428.3 62.1% 68% (many diffs)
28 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... XM_006501429.3 62.1% 68% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1887
ORF length:
1821
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agataatctc agtgatacct tgaagaagct gaagataaca gctgttgaca
121agactgagga tagtttagaa ggatgcttgg attgtctgct tcaagccctg gctcaaaata
181atacggaaac aagtgaaaaa atccaagcaa gtggaatact tcagctgttt gcaactctgt
241tgactccaca gtcttcctgc aaagccaaag tagctaacat catagcagaa gtagccaaaa
301atgagtttat gcgaattcca tgtgtggatg ctggattgat ttcaccactg gtgcagctgc
361taaatagcaa agaccaggaa gtgctgcttc aaacgggcag ggctctagga aacatatgtt
421acgatagcca tgagggcaga agtgcagttg accaagcagg tggtgcacag attgtaattg
481accatttaag gtcactgtgc agtataacag atcccgccaa tgagaagctc ttgactgtct
541tttgtggcat gctgatgaac tatagcaatg agaatgattc gcttcaagct cagcttatca
601atatgggtgt tattcctacc ttagtgaaat tactgggcat ccactgccaa aatgcagctc
661ttacagaaat gtgtcttgtt gcatttggta atttagcaga acttgagtca agtaaagaac
721agtttgccag tacaaacatt gctgaagagc tagtaaaact cttcaagaaa caaatagaac
781atgataagag agaaatgatt tttgaagttc ttgctccatt ggcagaaaat gatgctatta
841aactacagct ggttgaagca ggcctagtag agtgtctact agagattgtt cagcaaaaag
901tggatagtga caaagaagat gatattactg agctcaaaac tggttcagat ctcatggttt
961tattacttct tggagatgaa tccatgcaga agttatttga aggaggaaaa ggtagtgtat
1021ttcaaagggt actctcttgg atcccatcaa ataaccacca gctacagctt gctggagcat
1081tggcaattgc aaattttgcc agaaatgatg caaattgtat tcatatggta gacaatggga
1141ttgtagaaaa acttatggat ttactggaca gacatgtaga agatggaaat gtaacagtac
1201agcatgcagc actaagtgcc ctcagaaacc tggccattcc agttataaat aaagcaaaga
1261tgttatcagc tggggtcaca gaggcagttt tgaaatttct taaatctgaa atgcctcctg
1321ttcagttcaa acttctggga acattaagaa tgttaataga tgcacaagaa gctgctgaac
1381aattgggaaa gaatgttaag ttagtggagc gtttggtgga atggtgtgaa gccaaagatc
1441atgctggtgt gatgggggag tcaaacagac tgctgtctgc ccttatacga cacagtaaat
1501caaaagatgt aattaaaacc attgtgcaga gtggtggcat caagcatcta gttaccatgg
1561caactagtga acatgtaata atgcagaatg aagctcttgt tgctttggca ttaatagcag
1621ctttagaatt gggcactgct gagaaagatc tagaaagtgc taaacttgta cagattttac
1681atagactgct agcagatgag agaagtgctc ctgaaatcaa atataattcc atggtcctga
1741tatgtgctct tatgggatct gaatgtctac acaaggaagt acaggatttg gcttttctag
1801atgtcgtatc caaacttcgc agtcatgaga acaaaagtgt tgcccagcag gcctctctca
1861cagagcagag acttactgtg gaaagctnnc caactttctt gtacaaagtg gttggtaagc
1921ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
1981ggcgcgttaa gtcgacaatc aacctctgga tta

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