Construct: ORF ccsbBroad304_15607
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014772.1_s304c1, BRDN0000408821
- Derived from:
- ccsbBroadEn_15607
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCF4 (6925)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15607
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6925 | TCF4 | transcription factor 4 | NM_001369571.1 | 99.9% | 100% | 1929A>G |
| 2 | human | 6925 | TCF4 | transcription factor 4 | NM_001369572.1 | 99.9% | 100% | 1929A>G |
| 3 | human | 6925 | TCF4 | transcription factor 4 | NM_003199.3 | 99.9% | 100% | 1929A>G |
| 4 | human | 6925 | TCF4 | transcription factor 4 | NM_001369573.1 | 99.8% | 99.8% | 366_367insCAG;1926A>G |
| 5 | human | 6925 | TCF4 | transcription factor 4 | NM_001369574.1 | 99.8% | 99.8% | 1066_1067insCAG;1926A>G |
| 6 | human | 6925 | TCF4 | transcription factor 4 | NM_001083962.2 | 99.3% | 99.4% | 1634_1645del;1941A>G |
| 7 | human | 6925 | TCF4 | transcription factor 4 | NM_001369567.1 | 99.3% | 99.4% | 1634_1645del;1941A>G |
| 8 | human | 6925 | TCF4 | transcription factor 4 | NM_001369568.1 | 99.3% | 99.4% | 1634_1645del;1941A>G |
| 9 | human | 6925 | TCF4 | transcription factor 4 | NM_001330604.3 | 99.2% | 99.2% | 1066_1067insCAG;1631_1642del;1938A>G |
| 10 | human | 6925 | TCF4 | transcription factor 4 | NM_001369569.1 | 99.2% | 99.2% | 366_367insCAG;1631_1642del;1938A>G |
| 11 | human | 6925 | TCF4 | transcription factor 4 | NM_001369570.1 | 99.2% | 99.2% | 366_367insCAG;1631_1642del;1938A>G |
| 12 | human | 6925 | TCF4 | transcription factor 4 | NM_001243228.2 | 98.4% | 98.5% | 550_567del;1652_1663del;1959A>G |
| 13 | human | 6925 | TCF4 | transcription factor 4 | NM_001243230.1 | 96.9% | 96.8% | (many diffs) |
| 14 | human | 6925 | TCF4 | transcription factor 4 | NM_001306207.1 | 96.3% | 96.4% | 0_1ins72;1857A>G |
| 15 | human | 6925 | TCF4 | transcription factor 4 | NM_001348219.2 | 96.3% | 96.4% | 0_1ins72;1857A>G |
| 16 | human | 6925 | TCF4 | transcription factor 4 | NM_001369582.1 | 96.3% | 96.4% | 0_1ins72;1857A>G |
| 17 | human | 6925 | TCF4 | transcription factor 4 | NM_001369583.1 | 96.3% | 96.4% | 0_1ins72;1857A>G |
| 18 | human | 6925 | TCF4 | transcription factor 4 | NM_001348220.1 | 96.2% | 96.2% | 0_1ins72;294_295insCAG;1854A>G |
| 19 | human | 6925 | TCF4 | transcription factor 4 | NM_001369584.1 | 96.2% | 96.2% | 0_1ins72;294_295insCAG;1854A>G |
| 20 | human | 6925 | TCF4 | transcription factor 4 | NM_001369585.1 | 96.2% | 96.2% | 0_1ins72;294_295insCAG;1854A>G |
| 21 | human | 6925 | TCF4 | transcription factor 4 | XM_017025950.2 | 96% | 96.1% | (many diffs) |
| 22 | human | 6925 | TCF4 | transcription factor 4 | NM_001243227.2 | 95.7% | 95.8% | 0_1ins72;1562_1573del;1869A>G |
| 23 | human | 6925 | TCF4 | transcription factor 4 | NM_001348217.1 | 95.7% | 95.8% | 0_1ins72;1562_1573del;1869A>G |
| 24 | human | 6925 | TCF4 | transcription factor 4 | NM_001348218.2 | 95.7% | 95.8% | 0_1ins72;1562_1573del;1869A>G |
| 25 | human | 6925 | TCF4 | transcription factor 4 | NM_001369575.1 | 95.7% | 95.8% | 0_1ins72;1562_1573del;1869A>G |
| 26 | human | 6925 | TCF4 | transcription factor 4 | NM_001369586.1 | 95.6% | 95.5% | (many diffs) |
| 27 | human | 6925 | TCF4 | transcription factor 4 | NM_001369576.1 | 95.6% | 95.6% | (many diffs) |
| 28 | human | 6925 | TCF4 | transcription factor 4 | NM_001369577.1 | 95.6% | 95.6% | (many diffs) |
| 29 | human | 6925 | TCF4 | transcription factor 4 | NM_001369578.1 | 95.6% | 95.6% | (many diffs) |
| 30 | human | 6925 | TCF4 | transcription factor 4 | NM_001369579.1 | 95.6% | 95.6% | (many diffs) |
| 31 | human | 6925 | TCF4 | transcription factor 4 | NM_001369580.1 | 95.6% | 95.6% | (many diffs) |
| 32 | human | 6925 | TCF4 | transcription factor 4 | NM_001369581.1 | 95.6% | 95.6% | (many diffs) |
| 33 | human | 6925 | TCF4 | transcription factor 4 | XM_006722538.3 | 95.6% | 95.6% | (many diffs) |
| 34 | human | 6925 | TCF4 | transcription factor 4 | NM_001243231.2 | 93.4% | 92.6% | (many diffs) |
| 35 | human | 6925 | TCF4 | transcription factor 4 | NM_001348211.2 | 92.8% | 92.1% | (many diffs) |
| 36 | human | 6925 | TCF4 | transcription factor 4 | XM_017025951.2 | 92.7% | 91.9% | (many diffs) |
| 37 | human | 6925 | TCF4 | transcription factor 4 | XM_024451241.1 | 87.5% | 87.6% | 0_1ins237;1397_1408del;1704A>G |
| 38 | human | 6925 | TCF4 | transcription factor 4 | XM_017025953.2 | 86.6% | 82.8% | (many diffs) |
| 39 | human | 6925 | TCF4 | transcription factor 4 | NM_001306208.1 | 86.5% | 82.7% | (many diffs) |
| 40 | human | 6925 | TCF4 | transcription factor 4 | NM_001243226.3 | 86.2% | 86.2% | 1_306del;1940_1951del;2247A>G |
| 41 | human | 6925 | TCF4 | transcription factor 4 | XM_005266749.4 | 86.1% | 82.4% | (many diffs) |
| 42 | human | 6925 | TCF4 | transcription factor 4 | NM_001243232.1 | 86% | 82.2% | (many diffs) |
| 43 | human | 6925 | TCF4 | transcription factor 4 | XM_017025952.2 | 85.8% | 82.1% | (many diffs) |
| 44 | human | 6925 | TCF4 | transcription factor 4 | NM_001243233.2 | 80.4% | 80.5% | 0_1ins390;1539A>G |
| 45 | human | 6925 | TCF4 | transcription factor 4 | NM_001348212.2 | 80.4% | 80.5% | 0_1ins390;1539A>G |
| 46 | human | 6925 | TCF4 | transcription factor 4 | XM_017025956.2 | 80.4% | 80.5% | 0_1ins390;1539A>G |
| 47 | human | 6925 | TCF4 | transcription factor 4 | NM_001330605.3 | 79.9% | 80% | 0_1ins390;1244_1255del;1551A>G |
| 48 | human | 6925 | TCF4 | transcription factor 4 | NM_001348213.2 | 79.9% | 80% | 0_1ins390;1244_1255del;1551A>G |
| 49 | human | 6925 | TCF4 | transcription factor 4 | XM_005266752.5 | 79.9% | 80% | 0_1ins390;1244_1255del;1551A>G |
| 50 | human | 6925 | TCF4 | transcription factor 4 | XM_005266755.5 | 79.9% | 80% | 0_1ins390;1244_1255del;1551A>G |
| 51 | human | 6925 | TCF4 | transcription factor 4 | XM_017025954.2 | 79.8% | 79.8% | (many diffs) |
| 52 | human | 6925 | TCF4 | transcription factor 4 | NM_001243236.2 | 75% | 72.4% | (many diffs) |
| 53 | human | 6925 | TCF4 | transcription factor 4 | NM_001348216.2 | 74.6% | 71.9% | (many diffs) |
| 54 | human | 6925 | TCF4 | transcription factor 4 | NM_001243235.2 | 74.3% | 71.7% | (many diffs) |
| 55 | human | 6925 | TCF4 | transcription factor 4 | NM_001348214.2 | 74.1% | 71.6% | (many diffs) |
| 56 | human | 6925 | TCF4 | transcription factor 4 | NM_001243234.2 | 73.8% | 71.3% | (many diffs) |
| 57 | human | 6925 | TCF4 | transcription factor 4 | XM_005266761.4 | 73.7% | 71.2% | (many diffs) |
| 58 | human | 6925 | TCF4 | transcription factor 4 | NM_001348215.2 | 67.1% | 67.2% | 0_1ins648;986_997del;1293A>G |
| 59 | mouse | 21413 | Tcf4 | transcription factor 4 | NM_001083967.1 | 92.4% | 98% | (many diffs) |
| 60 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525750.3 | 92.4% | 98% | (many diffs) |
| 61 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317857.1 | 92.4% | 98% | (many diffs) |
| 62 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525751.3 | 92.3% | 97.9% | (many diffs) |
| 63 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525752.3 | 92.3% | 97.9% | (many diffs) |
| 64 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317858.1 | 92.1% | 97.7% | (many diffs) |
| 65 | mouse | 21413 | Tcf4 | transcription factor 4 | NM_013685.2 | 91.9% | 97.4% | (many diffs) |
| 66 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525746.3 | 91.9% | 97.4% | (many diffs) |
| 67 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525747.3 | 91.9% | 97.4% | (many diffs) |
| 68 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525748.3 | 91.7% | 97.3% | (many diffs) |
| 69 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525749.3 | 91.7% | 97.3% | (many diffs) |
| 70 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317854.1 | 91.7% | 97.3% | (many diffs) |
| 71 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317855.1 | 91.7% | 97.3% | (many diffs) |
| 72 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317856.1 | 91.6% | 97.1% | (many diffs) |
| 73 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525753.3 | 88.3% | 93.8% | (many diffs) |
| 74 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317859.1 | 88.1% | 93.7% | (many diffs) |
| 75 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317861.1 | 73.7% | 79% | (many diffs) |
| 76 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525757.3 | 73.3% | 78.5% | (many diffs) |
| 77 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525759.3 | 73.3% | 78.5% | (many diffs) |
| 78 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317860.1 | 73.3% | 78.5% | (many diffs) |
| 79 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317863.1 | 73.3% | 78.5% | (many diffs) |
| 80 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525762.3 | 68.8% | 70.4% | (many diffs) |
| 81 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525760.3 | 68.4% | 70% | (many diffs) |
| 82 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525766.3 | 68.3% | 69.8% | (many diffs) |
| 83 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525763.3 | 68.2% | 71% | (many diffs) |
| 84 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525761.3 | 67.8% | 70.6% | (many diffs) |
| 85 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317862.1 | 61.6% | 66.4% | (many diffs) |
| 86 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525764.3 | 61.2% | 66% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2070
- ORF length:
- 2001
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcatcaccaa cagcgaatgg ctgccttagg gacggacaaa gagctgagtg 121 atttactgga tttcagtgcg atgttttcac ctcctgtgag cagtgggaaa aatggaccaa 181 cttctttggc aagtggacat tttactggct caaatgtaga agacagaagt agctcagggt 241 cctgggggaa tggaggacat ccaagcccgt ccaggaacta tggagatggg actccctatg 301 accacatgac cagcagggac cttgggtcac atgacaatct ctctccacct tttgtcaatt 361 ccagaataca aagtaaaaca gaaaggggct catactcatc ttatgggaga gaatcaaact 421 tacagggttg ccaccagcag agtctccttg gaggtgacat ggatatgggc aacccaggaa 481 ccctttcgcc caccaaacct ggttcccagt actatcagta ttctagcaat aatccccgaa 541 ggaggcctct tcacagtagt gccatggagg tacagacaaa gaaagttcga aaagttcctc 601 caggtttgcc atcttcagtc tatgctccat cagcaagcac tgccgactac aatagggact 661 cgccaggcta tccttcctcc aaaccagcaa ccagcacttt ccctagctcc ttcttcatgc 721 aagatggcca tcacagcagt gacccttgga gctcctccag tgggatgaat cagcctggct 781 atgcaggaat gttgggcaac tcttctcata ttccacagtc cagcagctac tgtagcctgc 841 atccacatga acgtttgagc tatccatcac actcctcagc agacatcaat tccagtcttc 901 ctccgatgtc cactttccat cgtagtggta caaaccatta cagcacctct tcctgtacgc 961 ctcctgccaa cgggacagac agtataatgg caaatagagg aagcggggca gccggcagct 1021 cccagactgg agatgctctg gggaaagcac ttgcttcgat ctattctcca gatcacacta 1081 acaacagctt ttcatcaaac ccttcaactc ctgttggctc tcctccatct ctctcagcag 1141 gcacagctgt ttggtctaga aatggaggac aggcctcatc gtctcctaat tatgaaggac 1201 ccttacactc tttgcaaagc cgaattgaag atcgtttaga aagactggat gatgctattc 1261 atgttctccg gaaccatgca gtgggcccat ccacagctat gcctggtggt catggggaca 1321 tgcatggaat cattggacct tctcataatg gagccatggg tggtctgggc tcagggtatg 1381 gaaccggcct tctttcagcc aacagacatt cactcatggt ggggacccat cgtgaagatg 1441 gcgtggccct gagaggcagc cattctcttc tgccaaacca ggttccggtt ccacagcttc 1501 ctgtccagtc tgcgacttcc cctgacctga acccacccca ggacccttac agaggcatgc 1561 caccaggact acaggggcag agtgtctcct ctggcagctc tgagatcaaa tccgatgacg 1621 agggtgatga gaacctgcaa gacacgaaat cttcggagga caagaaatta gatgacgaca 1681 agaaggatat caaatcaatt actagcaata atgacgatga ggacctgaca ccagagcaga 1741 aggcagagcg tgagaaggag cggaggatgg ccaacaatgc ccgagagcgt ctgcgggtcc 1801 gtgacatcaa cgaggctttc aaagagctcg gccgcatggt gcagctccac ctcaagagtg 1861 acaagcccca gaccaagctc ctgatcctcc accaggcggt ggccgtcatc ctcagtctgg 1921 agcagcaagt ccgagaaagg aatctgaatc cgaaagctgc gtgtctgaaa agaagggagg 1981 aagagaaggt gtcctcggag cctccccctc tctccttggc cggcccacac cctggaatgg 2041 gagacgcatc gaatcacatg ggacagatgt nnccaacttt cttgtacaaa gtggttggta 2101 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2161 ggtggcgcgt taagtcgaca atcaacctct ggatta