Construct: ORF ccsbBroad304_15681

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004991.1_s304c1, BRDN0000398761
Derived from:
ccsbBroadEn_15681
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SFI1 (9814)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15681
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9814 SFI1 SFI1 centrin binding protein XM_024452311.1 45.7% 42.1% (many diffs)
2 human 9814 SFI1 SFI1 centrin binding protein XM_024452310.1 43.6% 40.2% (many diffs)
3 human 9814 SFI1 SFI1 centrin binding protein XM_024452309.1 43.4% 40% (many diffs)
4 human 9814 SFI1 SFI1 centrin binding protein XM_011530587.3 38.1% 35.1% (many diffs)
5 human 9814 SFI1 SFI1 centrin binding protein XM_017029159.2 38.1% 35.1% (many diffs)
6 human 9814 SFI1 SFI1 centrin binding protein XM_017029157.1 25% 23.1% (many diffs)
7 human 9814 SFI1 SFI1 centrin binding protein XM_017029156.2 24.5% 22.6% (many diffs)
8 human 9814 SFI1 SFI1 centrin binding protein NM_001258327.2 24.1% 22.2% (many diffs)
9 human 9814 SFI1 SFI1 centrin binding protein XM_017029155.1 23.9% 22% (many diffs)
10 human 9814 SFI1 SFI1 centrin binding protein NM_001258326.2 23.8% 22% (many diffs)
11 human 9814 SFI1 SFI1 centrin binding protein XM_006724390.3 23.8% 22% (many diffs)
12 human 9814 SFI1 SFI1 centrin binding protein XM_017029152.1 23.5% 21.6% (many diffs)
13 human 9814 SFI1 SFI1 centrin binding protein NM_001258325.1 23.3% 21.5% (many diffs)
14 human 9814 SFI1 SFI1 centrin binding protein XM_017029154.2 23.3% 21.5% (many diffs)
15 human 9814 SFI1 SFI1 centrin binding protein XM_017029153.1 23.2% 21.4% (many diffs)
16 human 9814 SFI1 SFI1 centrin binding protein XM_017029146.2 23% 21.2% (many diffs)
17 human 9814 SFI1 SFI1 centrin binding protein XM_011530582.2 22.9% 21.1% (many diffs)
18 human 9814 SFI1 SFI1 centrin binding protein XM_011530583.2 22.9% 21.1% (many diffs)
19 human 9814 SFI1 SFI1 centrin binding protein NM_014775.4 22.8% 21.1% (many diffs)
20 human 9814 SFI1 SFI1 centrin binding protein XM_017029151.2 22.8% 21.1% (many diffs)
21 human 9814 SFI1 SFI1 centrin binding protein XM_024452308.1 22.8% 21% (many diffs)
22 human 9814 SFI1 SFI1 centrin binding protein XM_017029145.1 22.8% 21% (many diffs)
23 human 9814 SFI1 SFI1 centrin binding protein XM_017029149.2 22.7% 20.9% (many diffs)
24 human 9814 SFI1 SFI1 centrin binding protein XM_017029150.2 22.7% 20.9% (many diffs)
25 human 9814 SFI1 SFI1 centrin binding protein XM_017029147.1 22.7% 20.9% (many diffs)
26 human 9814 SFI1 SFI1 centrin binding protein XM_017029148.1 22.7% 20.9% (many diffs)
27 human 9814 SFI1 SFI1 centrin binding protein XM_017029141.2 22.5% 20.8% (many diffs)
28 human 9814 SFI1 SFI1 centrin binding protein XM_017029140.1 22.4% 20.6% (many diffs)
29 human 9814 SFI1 SFI1 centrin binding protein XM_017029144.1 22.3% 20.6% (many diffs)
30 human 9814 SFI1 SFI1 centrin binding protein NM_001007467.3 22.3% 20.5% (many diffs)
31 human 9814 SFI1 SFI1 centrin binding protein XM_017029139.2 22.3% 20.5% (many diffs)
32 human 9814 SFI1 SFI1 centrin binding protein XM_017029143.1 22.3% 20.5% (many diffs)
33 human 9814 SFI1 SFI1 centrin binding protein XM_017029142.1 22.1% 20.4% (many diffs)
34 human 9814 SFI1 SFI1 centrin binding protein XM_011530581.3 22% 20.3% (many diffs)
35 human 9814 SFI1 SFI1 centrin binding protein XM_011530580.2 22% 20.2% (many diffs)
36 human 9814 SFI1 SFI1 centrin binding protein XM_011530579.3 21.9% 20.2% (many diffs)
37 human 9814 SFI1 SFI1 centrin binding protein XM_011530576.2 21.8% 20.1% (many diffs)
38 human 9814 SFI1 SFI1 centrin binding protein XM_011530577.2 21.8% 20.1% (many diffs)
39 human 9814 SFI1 SFI1 centrin binding protein XM_005261868.2 21.8% 20.1% (many diffs)
40 human 9814 SFI1 SFI1 centrin binding protein XM_011530575.2 21.7% 20% (many diffs)
41 human 9814 SFI1 SFI1 centrin binding protein XM_011530574.3 21.5% 19.8% (many diffs)
42 human 9814 SFI1 SFI1 centrin binding protein XM_011530570.2 21.4% 19.7% (many diffs)
43 human 9814 SFI1 SFI1 centrin binding protein XM_011530571.2 21.4% 19.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
948
ORF length:
882
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccaggcccac cccagcttac ttgctgacct tggcctcgcc tttcctgact
121ttcagtctcc ctataaaatt ggagatccca cagagatctc agacccccct gcagcccctg
181gtcagcccag gggaacagac cccatgtttc tttccagcaa cactgcccac tcagcgagga
241agcagccgcg acgcccacac ttcctgttgg agcctgcgca gagccagagg cctcagaagc
301cacaggaaca tggcctaggc atggctcagc cagcagcccc ctccctgacg cggcccttcc
361tggcagaggc cccgacagca ctggtcccac acagccccct gcctggggcc ctgtcaagcg
421cccctggccc gaagcagccc ccgacggcaa gcacaggccc ggagctgctg ctgctgcctc
481tttcctcctt catgccctgc ggggcggctg caccagccag ggtgtcagca cagcgggcta
541ctcctaggga taagcccccg gtcccctcat ccctggccag tgtccctgac ccccatctac
601tccttcctgg ggacttctca gccaccaggg ctgggcctgg actttcaact gcaggcagcc
661tggaccttga ggctgaactt gaggagatcc agcagcaact actgcactac cagaccacca
721agcagaacct ctggtcctgt cggcggcaag cgagcagcct gcgcaggtgg ctggagctga
781acagagagga gccggggcct gaggaccagg aagtagagca gcaggtgcag aaagagctgg
841aacaggtgga aatgcagatc cagctgctgg cagaggagct ccaggctcag cgccagccca
901ttggcgcctg cgttgcccgc atccaggccc tgcggcaggc cctgtgctnn ccaactttct
961tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1021aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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