Construct: ORF ccsbBroad304_15790

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017183.1_s304c1, BRDN0000398776
Derived from:
ccsbBroadEn_15790
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BBS9 (27241)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15790
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27241 BBS9 Bardet-Biedl syndrome 9 XM_017011994.2 52.2% 50% (many diffs)
2 human 27241 BBS9 Bardet-Biedl syndrome 9 XR_001744634.2 43.6% (many diffs)
3 human 27241 BBS9 Bardet-Biedl syndrome 9 XM_005249701.3 43% 41.2% (many diffs)
4 human 27241 BBS9 Bardet-Biedl syndrome 9 XM_011515270.3 41.9% 40.2% (many diffs)
5 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348042.2 36.3% 34.9% (many diffs)
6 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348040.3 36.2% 34.7% (many diffs)
7 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_014451.3 36.1% 34.7% (many diffs)
8 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001033604.1 35.9% 34.5% (many diffs)
9 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001362679.1 35.1% 33.7% (many diffs)
10 human 27241 BBS9 Bardet-Biedl syndrome 9 XM_011515267.3 35.1% 33.7% (many diffs)
11 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001033605.1 34.7% 33.3% (many diffs)
12 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348043.3 34.5% 33.2% (many diffs)
13 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348036.1 34.5% 33.1% (many diffs)
14 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_198428.3 34.5% 33.1% (many diffs)
15 human 27241 BBS9 Bardet-Biedl syndrome 9 XM_011515266.3 33.9% 32.5% (many diffs)
16 human 27241 BBS9 Bardet-Biedl syndrome 9 XM_017011990.1 33.9% 32.5% (many diffs)
17 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348041.4 33.7% 32.4% (many diffs)
18 human 27241 BBS9 Bardet-Biedl syndrome 9 XM_011515265.2 33.7% 32.4% (many diffs)
19 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348039.2 32.2% 30.7% (many diffs)
20 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348038.2 30.9% 29.5% (many diffs)
21 human 27241 BBS9 Bardet-Biedl syndrome 9 XM_011515269.2 30.1% 28.8% (many diffs)
22 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348037.2 29.3% 27.8% (many diffs)
23 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348044.2 28.4% 26.9% (many diffs)
24 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348045.2 27.2% 25.8% (many diffs)
25 human 27241 BBS9 Bardet-Biedl syndrome 9 NM_001348046.2 27.2% 25.8% (many diffs)
26 human 27241 BBS9 Bardet-Biedl syndrome 9 NR_145411.1 23.7% (many diffs)
27 human 27241 BBS9 Bardet-Biedl syndrome 9 NR_145412.1 23.1% (many diffs)
28 human 27241 BBS9 Bardet-Biedl syndrome 9 NR_145413.2 22% (many diffs)
29 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... XM_006510421.3 59.6% 60.5% (many diffs)
30 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... NM_178415.1 30% 30.7% (many diffs)
31 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... NM_181316.4 30% 30.7% (many diffs)
32 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... XM_006510419.3 30% 30.7% (many diffs)
33 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... XM_006510415.3 29.9% 30.5% (many diffs)
34 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... XM_006510416.3 29.9% 30.5% (many diffs)
35 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... XM_006510417.3 29.9% 30.5% (many diffs)
36 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... XM_006510418.3 29.9% 30.5% (many diffs)
37 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... XM_006510420.3 27% 27.4% (many diffs)
38 mouse 319845 Bbs9 Bardet-Biedl syndrome 9 (hu... XM_011242551.2 20.1% 20% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
996
ORF length:
930
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg atacctaagg atctttagcc cccatcctgc aaaaacagga gatggagctc
121aagccgaaga tttgcttcta gaagtggatc tacgagatcc agtacttcaa gtggaagtag
181gaaagtttgt ttcaggtacc gaaatgctac atttggctgt gttacattct agaaaacttt
241gtgtctactc tgtctcagga accttgggta atgtggaaca tgggaaccaa tgtcagatga
301aattgatgta tgaacataat cttcagagaa cagcctgcaa tatgacctat ggatcatttg
361gtggtgtaaa aggtcgagat ttaatttgca tccagtctat ggatgggatg ctgatggtat
421ttgagcagga gagctatgct tttggaagat ttctccctgg ctttcttctg cctggtcctc
481ttgcctacag ttcccgtaca gattccttcc ttactgtctc ttcctgccaa caagtggaaa
541gttataagta ccaggtactt gcttttgcaa cagatgcaga taaaaggcag gagactgaac
601agcaaaaact tggttctgga aaaagactag ttgtggattg gactctaaat attggagagc
661aagcccttga catatgtatt gtctctttca atcagtcggc atcctctgtt tttgttcttg
721gtgagagaaa ctttttttgc cttaaggata atggacaaat tcgattcatg aagaagcttg
781attggagccc aagttgtttt ctgccatatt gctcagtttc tgaaggaaca ataaatactt
841tgattggaaa tcataataac atgctgcata tttatcaaga tgtgacactg aagtgggcca
901cccaacttcc ccacattcct gtagcagtaa gagtgggctg tttgcaattt tctttatgga
961agcatttgtt gcctcgatct tccactttag aaaaatnncc aactttcttg tacaaagtgg
1021ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1081ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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