Construct: ORF ccsbBroad304_16137
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005735.1_s304c1, BRDN0000404831
- Derived from:
- ccsbBroadEn_16137
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRMD3 (257019)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16137
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244962.2 | 100% | 100% | |
| 2 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_017014591.2 | 83.8% | 82.2% | (many diffs) |
| 3 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244961.1 | 52.8% | 46% | (many diffs) |
| 4 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_017014589.1 | 47.5% | 40.5% | (many diffs) |
| 5 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_024447487.1 | 38.4% | 32.7% | (many diffs) |
| 6 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_024447488.1 | 38.4% | 32.7% | (many diffs) |
| 7 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_024447489.1 | 38.4% | 32.7% | (many diffs) |
| 8 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244960.1 | 34.8% | 29.7% | (many diffs) |
| 9 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244959.2 | 34.6% | 30.2% | (many diffs) |
| 10 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_017014588.1 | 34.6% | 29.5% | (many diffs) |
| 11 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_174938.6 | 32.3% | 27.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca ggccgaggag accaagggga ttggaataac cagtatggca aaatgcttgg 121 tcaagatcca gactcgacga agcctccaat tgcacatggt gaaccactgc aacagtaatg 181 tgtttgttcg tctgctgaga ctcggctcca aggtcacagc tcgtaacact ggtgttccat 241 tgcctaaaga ggagaacatt tctgctccct tgatctccag ctccccagtg aaggcagccc 301 gggagtatga agatccccct agtgaagagg aagataaaat aaaagaagaa cctttaacca 361 tctctgaact agtgtacaac ccaagtgcca gcctgctccc cacccctgtg gatgacgatg 421 agattgacat gctctttgac tgtccttcta ggcttgagtt ggaaagagaa gacacagatt 481 catttgagga tctggaagca gatgaaaacg cctttttgat tgctgaagaa gaggagctga 541 aggaggctcg ccgtgctttg tcgtggagct atgacattct gactggccat attcgggtga 601 acccactggt caagagtttt tccaggctcc ttgtggtggg cctgggactg ctgctctttg 661 tatttcccct gctcctcctc cttttggagt cagtctccat gcagtnncca actttcttgt 721 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 781 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a