Construct: ORF ccsbBroad304_16175
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019063.1_s304c1, BRDN0000464784
- Derived from:
- ccsbBroadEn_16175
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRAF (673)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16175
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_004333.4:c.1799T>A | 100% | 100% | |
| 2 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_004333.6 | 99.9% | 99.8% | 1799T>A |
| 3 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001354609.2 | 99.5% | 99.2% | (many diffs) |
| 4 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001374244.1 | 94.9% | 94.9% | 1175_1294del;1919T>A |
| 5 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001374258.1 | 94.5% | 94.2% | (many diffs) |
| 6 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XM_017012559.1 | 89.2% | 87.6% | (many diffs) |
| 7 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XR_001744857.1 | 48.1% | (many diffs) | |
| 8 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XR_001744858.1 | 22.1% | (many diffs) | |
| 9 | mouse | 109880 | Braf | Braf transforming gene | XM_006505359.3 | 82.4% | 86.5% | (many diffs) |
| 10 | mouse | 109880 | Braf | Braf transforming gene | XM_006505358.3 | 82.1% | 86.1% | (many diffs) |
| 11 | mouse | 109880 | Braf | Braf transforming gene | XM_011241139.2 | 81.8% | 85.8% | (many diffs) |
| 12 | mouse | 109880 | Braf | Braf transforming gene | NM_139294.5 | 80.9% | 84.8% | (many diffs) |
| 13 | mouse | 109880 | Braf | Braf transforming gene | XM_011241138.2 | 80.7% | 84.6% | (many diffs) |
| 14 | mouse | 109880 | Braf | Braf transforming gene | XM_011241136.2 | 80.6% | 84.5% | (many diffs) |
| 15 | mouse | 109880 | Braf | Braf transforming gene | XM_011241137.1 | 80% | 83.9% | (many diffs) |
| 16 | mouse | 109880 | Braf | Braf transforming gene | XM_006505355.3 | 78.4% | 82.3% | (many diffs) |
| 17 | mouse | 109880 | Braf | Braf transforming gene | XM_011241135.2 | 78.2% | 82% | (many diffs) |
| 18 | mouse | 109880 | Braf | Braf transforming gene | XM_006505353.3 | 77.4% | 81.2% | (many diffs) |
| 19 | mouse | 109880 | Braf | Braf transforming gene | XM_011241134.2 | 77.2% | 80.9% | (many diffs) |
| 20 | mouse | 109880 | Braf | Braf transforming gene | XM_011241140.2 | 56.8% | 59.8% | (many diffs) |
| 21 | mouse | 109880 | Braf | Braf transforming gene | XR_001785085.1 | 43.4% | (many diffs) | |
| 22 | mouse | 109880 | Braf | Braf transforming gene | XR_001785086.1 | 39.9% | (many diffs) | |
| 23 | mouse | 109880 | Braf | Braf transforming gene | XR_001785087.1 | 38.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2367
- ORF length:
- 2298
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgctg agcggtggcg gtggtggcgg cgcggagccg ggccaggctc 121 tgttcaacgg ggacatggag cccgaggccg gcgccggcgc cggcgccgcg gcctcttcgg 181 ctgcggaccc tgccattccg gaggaggtgt ggaatatcaa acaaatgatt aagttgacac 241 aggaacatat agaggcccta ttggacaaat ttggtgggga gcataatcca ccatcaatat 301 atctggaggc ctatgaagaa tacaccagca agctagatgc actccaacaa agagaacaac 361 agttattgga atctctgggg aacggaactg atttttctgt ttctagctct gcatcaatgg 421 ataccgttac atcttcttcc tcttctagcc tttcagtgct accttcatct ctttcagttt 481 ttcaaaatcc cacagatgtg gcacggagca accccaagtc accacaaaaa cctatcgtta 541 gagtcttcct gcccaacaaa cagaggacag tggtacctgc aaggtgtgga gttacagtcc 601 gagacagtct aaagaaagca ctgatgatga gaggtctaat cccagagtgc tgtgctgttt 661 acagaattca ggatggagag aagaaaccaa ttggttggga cactgatatt tcctggctta 721 ctggagaaga attgcatgtg gaagtgttgg agaatgttcc acttacaaca cacaactttg 781 tacgaaaaac gtttttcacc ttagcatttt gtgacttttg tcgaaagctg cttttccagg 841 gtttccgctg tcaaacatgt ggttataaat ttcaccagcg ttgtagtaca gaagttccac 901 tgatgtgtgt taattatgac caacttgatt tgctgtttgt ctccaagttc tttgaacacc 961 acccaatacc acaggaagag gcgtccttag cagagactgc cctaacatct ggatcatccc 1021 cttccgcacc cgcctcggac tctattgggc cccaaattct caccagtccg tctccttcaa 1081 aatccattcc aattccacag cccttccgac cagcagatga agatcatcga aatcaatttg 1141 ggcaacgaga ccgatcctca tcagctccca atgtgcatat aaacacaata gaacctgtca 1201 atattgatga cttgattaga gaccaaggat ttcgtggtga tggaggatca accacaggtt 1261 tgtctgctac cccccctgcc tcattacctg gctcactaac taacgtgaaa gccttacaga 1321 aatctccagg acctcagcga gaaaggaagt catcttcatc ctcagaagac aggaatcgaa 1381 tgaaaacact tggtagacgg gactcgagtg atgattggga gattcctgat gggcagatta 1441 cagtgggaca aagaattgga tctggatcat ttggaacagt ctacaaggga aagtggcatg 1501 gtgatgtggc agtgaaaatg ttgaatgtga cagcacctac acctcagcag ttacaagcct 1561 tcaaaaatga agtaggagta ctcaggaaaa cacgacatgt gaatatccta ctcttcatgg 1621 gctattccac aaagccacaa ctggctattg ttacccagtg gtgtgagggc tccagcttgt 1681 atcaccatct ccatatcatt gagaccaaat ttgagatgat caaacttata gatattgcac 1741 gacagactgc acagggcatg gattacttac acgccaagtc aatcatccac agagacctca 1801 agagtaataa tatatttctt catgaagacc tcacagtaaa aataggtgat tttggtctag 1861 ctacagagaa atctcgatgg agtgggtccc atcagtttga acagttgtct ggatccattt 1921 tgtggatggc accagaagtc atcagaatgc aagataaaaa tccatacagc tttcagtcag 1981 atgtatatgc atttggaatt gttctgtatg aattgatgac tggacagtta ccttattcaa 2041 acatcaacaa cagggaccag ataattttta tggtgggacg aggatacctg tctccagatc 2101 tcagtaaggt acggagtaac tgtccaaaag ccatgaagag attaatggca gagtgcctca 2161 aaaagaaaag agatgagaga ccactctttc cccaaattct cgcctctatt gagctgctgg 2221 cccgctcatt gccaaaaatt caccgcagtg catcagaacc ctccttgaat cgggctggtt 2281 tccaaacaga ggattttagt ctatatgctt gtgcttctcc aaaaacaccc atccaggcag 2341 ggggatatgg tgcgtttcct gtccacttgc caactttctt gtacaaagtg gttggtaagc 2401 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2461 ggcgcgttaa gtcgacaatc aacctctgga tta