Construct: ORF ccsbBroadEn_01248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008681.1_s300c1, BRDN0000391535
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POU6F1 (5463)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019529.1 | 100% | 100% | |
| 2 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019518.1 | 52% | 49.9% | (many diffs) |
| 3 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019517.1 | 51.8% | 49.5% | (many diffs) |
| 4 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019516.1 | 49.3% | 49.3% | 1_927del |
| 5 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NM_001330422.2 | 49.2% | 49.2% | 1_930del |
| 6 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019514.1 | 49.2% | 49.2% | 1_930del |
| 7 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019513.1 | 49.1% | 49% | 1_927del;1319_1324delGCCTAG |
| 8 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019508.1 | 49.1% | 48.9% | 1_930del;1322_1327delGCCTAG |
| 9 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019510.2 | 49.1% | 48.9% | 1_930del;1322_1327delGCCTAG |
| 10 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019511.1 | 49.1% | 48.9% | 1_930del;1322_1327delGCCTAG |
| 11 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019512.2 | 49.1% | 48.9% | 1_930del;1322_1327delGCCTAG |
| 12 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_024449026.1 | 49.1% | 48.9% | 1_930del;1322_1327delGCCTAG |
| 13 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_024449027.1 | 22.1% | 20.3% | (many diffs) |
| 14 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XM_017019519.1 | 21.4% | 21.2% | 1_930del;1321_1328delAGTTCGAG;1332_1333ins509 |
| 15 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_160430.1 | 17.2% | 1_1015del;1919_5232del | |
| 16 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_147049.2 | 17% | 1_1092del;1996_5309del | |
| 17 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_147052.2 | 16.6% | 1_1214del;2118_5431del | |
| 18 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_147050.2 | 16.5% | 1_1233del;2137_5450del | |
| 19 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_147046.2 | 16.4% | (many diffs) | |
| 20 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_147048.2 | 16.4% | (many diffs) | |
| 21 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_147051.2 | 16.3% | 1_1301del;2205_5518del | |
| 22 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_147047.2 | 16.3% | 1_1306del;2210_5523del | |
| 23 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_026893.3 | 16.3% | 1_1309del;2213_5526del | |
| 24 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | NR_160429.1 | 15.6% | (many diffs) | |
| 25 | human | 5463 | POU6F1 | POU class 6 homeobox 1 | XR_001748774.1 | 14.2% | 1_2140del;3044_6357del | |
| 26 | mouse | 19009 | Pou6f1 | POU domain, class 6, transc... | XM_030248419.1 | 79.9% | 86.7% | (many diffs) |
| 27 | mouse | 19009 | Pou6f1 | POU domain, class 6, transc... | XM_030248418.1 | 46% | 49.9% | (many diffs) |
| 28 | mouse | 19009 | Pou6f1 | POU domain, class 6, transc... | NM_001304965.1 | 45.9% | 49.8% | (many diffs) |
| 29 | mouse | 19009 | Pou6f1 | POU domain, class 6, transc... | XM_030248417.1 | 44.8% | 48.5% | (many diffs) |
| 30 | mouse | 19009 | Pou6f1 | POU domain, class 6, transc... | XM_006520609.4 | 43.4% | 47.1% | (many diffs) |
| 31 | mouse | 19009 | Pou6f1 | POU domain, class 6, transc... | XM_006520608.4 | 43.4% | 47% | (many diffs) |
| 32 | mouse | 19009 | Pou6f1 | POU domain, class 6, transc... | XM_030248416.1 | 43.4% | 47% | (many diffs) |
| 33 | mouse | 19009 | Pou6f1 | POU domain, class 6, transc... | XM_017316509.2 | 20.6% | 19.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 972
- ORF length:
- 903
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GCCAGGGATC AGCAGTCAGA TCCTCACCAA TGCTCAGGGA CAGGTTATTG 121 GAACCCTTCC ATGGGTAGTG AACTCAGCTA GTGTGGCGGC CCCAGCACCA GCCCAAAGCC 181 TGCAGGTCCA GGCCGTGACC CCCCAGCTGT TGTTGAACGC CCAGGGCCAG GTGATTGCGA 241 CCCTGGCTAG CAGCCCCCTG CCTCCACCTG TGGCTGTCCG GAAGCCAAGC ACACCTGAGT 301 CCCCTGCTAA GAGTGAGGTG CAGCCCATCC AGCCCACACC AACCGTGCCC CAGCCTGCTG 361 TGGTCATTGC CAGCCCAGCT CCAGCCGCCA AGCCATCTGC CTCTGCTCCT ATCCCAATTA 421 CCTGCTCAGA GACCCCCACC GTCAGCCAGT TGGTGTCCAA GCCACATACT CCAAGTCTGG 481 ATGAGGATGG GATCAACTTA GAAGAGATCC GGGAGTTTGC CAAGAACTTT AAGATCCGGC 541 GGCTCTCGCT GGGCCTTACA CAGACCCAGG TGGGTCAGGC TCTGACTGCA ACGGAAGGTC 601 CAGCCTACAG CCAGTCAGCC ATCTGCCGGT TCGAGAAGCT AGACATCACA CCCAAGAGTG 661 CCCAGAAGCT AAAGCCGGTG CTGGAAAAGT GGCTAAACGA AGCTGAACTG CGGAACCAGG 721 AAGGCCAGCA GAACCTGATG GAGTTTGTGG GAGGCGAGCC CTCCAAGAAA CGCAAACGCC 781 GCACCTCCTT CACCCCCCAG GCCATAGAGG CTCTCAATGC CTATTTTGAG AAGAACCCAC 841 TGCCCACAGG CCAGGAGATC ACTGAAATTG CTAAGGAGCT CAACTACGAC CGTGAGGTAG 901 TGCGGGTCTG GTTCTGCAAT CGGCGCCAGA CGCTCAAGAA CACCAGCAAG CTGAACGTCT 961 TTCAGATCCC TTTGCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat 1021 caatttgttg caacgaac