Construct: ORF ccsbBroadEn_01278

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008264.1_s300c1, BRDN0000385991
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKACB (5567)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5567 PRKACB protein kinase cAMP-activat... NM_207578.3 100% 100%
2 human 5567 PRKACB protein kinase cAMP-activat... XM_017001713.2 99.6% 99.6% 764_766delAGC
3 human 5567 PRKACB protein kinase cAMP-activat... XM_017001717.1 95.6% 95.7% (many diffs)
4 human 5567 PRKACB protein kinase cAMP-activat... NM_001300917.2 94.6% 93.7% (many diffs)
5 human 5567 PRKACB protein kinase cAMP-activat... XM_017001716.1 94.6% 96.1% (many diffs)
6 human 5567 PRKACB protein kinase cAMP-activat... XM_017001720.1 94.5% 94.5% 0_1ins40;2delT;6T>C
7 human 5567 PRKACB protein kinase cAMP-activat... XM_017001715.1 94.5% 94.5% (many diffs)
8 human 5567 PRKACB protein kinase cAMP-activat... XM_017001718.2 94.3% 93.4% (many diffs)
9 human 5567 PRKACB protein kinase cAMP-activat... XM_017001711.1 94.2% 94.2% (many diffs)
10 human 5567 PRKACB protein kinase cAMP-activat... XM_017001719.1 94.1% 94.1% (many diffs)
11 human 5567 PRKACB protein kinase cAMP-activat... XM_017001714.1 94.1% 94.2% (many diffs)
12 human 5567 PRKACB protein kinase cAMP-activat... XM_017001712.2 93.9% 94.6% (many diffs)
13 human 5567 PRKACB protein kinase cAMP-activat... XM_017001709.1 93.8% 93.9% (many diffs)
14 human 5567 PRKACB protein kinase cAMP-activat... XM_017001710.2 93.6% 94.2% (many diffs)
15 human 5567 PRKACB protein kinase cAMP-activat... XM_017001707.1 93.1% 93.1% (many diffs)
16 human 5567 PRKACB protein kinase cAMP-activat... XM_017001708.2 92.9% 93.5% (many diffs)
17 human 5567 PRKACB protein kinase cAMP-activat... XM_017001706.1 92.8% 92.8% (many diffs)
18 human 5567 PRKACB protein kinase cAMP-activat... NM_001300915.2 92.5% 93.1% (many diffs)
19 human 5567 PRKACB protein kinase cAMP-activat... NM_001300916.2 82.4% 80.9% (many diffs)
20 human 5567 PRKACB protein kinase cAMP-activat... XM_005271020.2 82.2% 80.6% (many diffs)
21 human 5567 PRKACB protein kinase cAMP-activat... NM_002731.3 73% 72.9% 766_781delinsA;786_1053delinsT
22 human 5567 PRKACB protein kinase cAMP-activat... XM_005271018.4 69.9% 69.8% (many diffs)
23 human 5567 PRKACB protein kinase cAMP-activat... XM_005271017.3 69.4% 70% (many diffs)
24 human 5567 PRKACB protein kinase cAMP-activat... XM_005271016.1 69.3% 69.1% (many diffs)
25 human 5567 PRKACB protein kinase cAMP-activat... NM_001242859.2 69.3% 69.1% (many diffs)
26 human 5567 PRKACB protein kinase cAMP-activat... XM_006710758.2 69.1% 69.2% (many diffs)
27 human 5567 PRKACB protein kinase cAMP-activat... NM_001242858.2 69.1% 68.3% (many diffs)
28 human 5567 PRKACB protein kinase cAMP-activat... XM_005271019.1 69% 68.9% (many diffs)
29 human 5567 PRKACB protein kinase cAMP-activat... XM_005271015.3 68.8% 69.5% (many diffs)
30 human 5567 PRKACB protein kinase cAMP-activat... NM_001242857.2 68.7% 68.4% (many diffs)
31 human 5567 PRKACB protein kinase cAMP-activat... NM_001242860.2 68.6% 68.6% (many diffs)
32 human 5567 PRKACB protein kinase cAMP-activat... NM_001242862.2 66.4% 63.2% (many diffs)
33 human 5567 PRKACB protein kinase cAMP-activat... NM_182948.4 62.8% 61.5% (many diffs)
34 human 5567 PRKACB protein kinase cAMP-activat... NM_001242861.2 59.9% 60% (many diffs)
35 human 5566 PRKACA protein kinase cAMP-activat... NM_002730.4 55.6% 68% (many diffs)
36 human 5566 PRKACA protein kinase cAMP-activat... NM_207518.3 53.4% 64.6% (many diffs)
37 mouse 18749 Prkacb protein kinase, cAMP depend... NM_011100.4 63.5% 70.6% (many diffs)
38 mouse 18749 Prkacb protein kinase, cAMP depend... NM_001164198.1 59.8% 66.9% (many diffs)
39 mouse 18749 Prkacb protein kinase, cAMP depend... NM_001164199.1 59.8% 66.6% (many diffs)
40 mouse 18749 Prkacb protein kinase, cAMP depend... NM_001164200.1 55.2% 59.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
837
ORF length:
771
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGG GAACGCGGCG ACCGCCAAGA AAGGCAGCGA GGTGGAGAGC GTGAAAGAGT
121TTCTAGCCAA AGCCAAAGAA GACTTTTTGA AAAAATGGGA GAATCCAACT CAGAATAATG
181CCGGACTTGA AGATTTTGAA AGGAAAAAAA CCCTTGGAAC AGGTTCATTT GGAAGAGTCA
241TGTTGGTAAA ACACAAAGCC ACTGAACAGT ATTATGCCAT GAAGATCTTA GATAAGCAGA
301AGGTTGTTAA ACTGAAGCAA ATAGAGCATA CTTTGAATGA GAAAAGAATA TTACAGGCAG
361TGAATTTTCC TTTCCTTGTT CGACTGGAGT ATGCTTTTAA GGATAATTCT AATTTATACA
421TGGTTATGGA ATATGTCCCT GGGGGTGAAA TGTTTTCACA TCTAAGAAGA ATTGGAAGGT
481TCAGTGAGCC CCATGCACGG TTCTATGCAG CTCAGATAGT GCTAACATTC GAGTACCTCC
541ATTCACTAGA CCTCATCTAC AGAGATCTAA AACCTGAAAA TCTCTTAATT GACCATCAAG
601GCTATATCCA GGTCACAGAC TTTGGGTTTG CCAAAAGAGT TAAAGGCAGA ACTTGGACAT
661TATGTGGAAC TCCAGAGTAT TTGGCTCCAG AAATAATTCT CAGCAAGGGC TACAATAAGG
721CAGTGGATTG GTGGGCATTA GGAGTGCTAA TCTATGAAAT GGCAGCTGGC TATCCCCCAT
781TCTTTGCAGA CCAACCAATT CAGATTTATG AAAAGATTGT TTCTGGAAAG AACTTTTGCC
841CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
901aac

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