Construct: ORF ccsbBroadEn_02088

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013883.1_s300c1, BRDN0000395678
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPF3 (9129)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9129 PRPF3 pre-mRNA processing factor 3 NM_004698.4 100% 100%
2 human 9129 PRPF3 pre-mRNA processing factor 3 XR_921998.3 86.4% 1_94del;1296_1297insTACAG;2139_2360del
3 human 9129 PRPF3 pre-mRNA processing factor 3 XR_002958012.1 86% (many diffs)
4 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737537.2 83.7% (many diffs)
5 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146768.1 81.9% 1_173del;2016_2025delGGTGAATGGG;2233_2501del
6 human 9129 PRPF3 pre-mRNA processing factor 3 XR_241103.3 81.6% 1_94del;1733_1734ins119;2025_2246del
7 human 9129 PRPF3 pre-mRNA processing factor 3 XR_921997.3 80.6% (many diffs)
8 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146766.1 80.3% 1_173del;1700_1757del;2281_2549del
9 human 9129 PRPF3 pre-mRNA processing factor 3 NM_001350529.1 80.2% 80.2% 0_1ins405
10 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146769.1 79.8% (many diffs)
11 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737541.2 79.5% 1_94del;1129_1130ins167;1977_2198del
12 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737536.2 79% (many diffs)
13 human 9129 PRPF3 pre-mRNA processing factor 3 XM_011510130.3 78.9% 78.9% 0_1ins432
14 human 9129 PRPF3 pre-mRNA processing factor 3 XM_017002790.1 78.9% 78.9% 0_1ins432
15 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146767.1 77.4% (many diffs)
16 human 9129 PRPF3 pre-mRNA processing factor 3 XR_002958009.1 69.5% 1_599del;2126_2183del;2707_2946del
17 human 9129 PRPF3 pre-mRNA processing factor 3 XM_011510131.3 62.8% 62.3% (many diffs)
18 human 9129 PRPF3 pre-mRNA processing factor 3 XM_011510132.3 60.9% 59% 1202_1203insTACAG;1248_1249ins796
19 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737540.2 59.8% (many diffs)
20 human 9129 PRPF3 pre-mRNA processing factor 3 XM_017002791.2 53% 51.2% 1035_1036ins167;1086_1087ins796
21 human 9129 PRPF3 pre-mRNA processing factor 3 XR_002958010.1 48.8% (many diffs)
22 mouse 70767 Prpf3 pre-mRNA processing factor 3 NM_001316751.1 92.1% 99.5% (many diffs)
23 mouse 70767 Prpf3 pre-mRNA processing factor 3 NM_027541.4 92.1% 99.5% (many diffs)
24 mouse 70767 Prpf3 pre-mRNA processing factor 3 XM_006502080.3 74.3% 79.5% (many diffs)
25 mouse 70767 Prpf3 pre-mRNA processing factor 3 XM_006502081.2 74% 79.7% (many diffs)
26 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_375580.2 56% (many diffs)
27 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_375581.2 55.2% (many diffs)
28 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783748.1 54.9% (many diffs)
29 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783747.1 54% (many diffs)
30 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783749.1 53.7% (many diffs)
31 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783750.1 53.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
67
ORF end:
2116
ORF length:
2049
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG GTACAAAAAA
61GTTGGCATGG CACTGTCAAA GAGGGAGCTG GATGAGCTGA AACCATGGAT AGAGAAGACA
121GTGAAGAGGG TCCTGGGTTT CTCAGAGCCT ACGGTGGTCA CAGCAGCATT GAACTGTGTG
181GGGAAGGGCA TGGACAAGAA GAAGGCAGCC GATCATCTGA AACCTTTTCT TGATGATTCT
241ACTCTCCGAT TTGTGGACAA ACTGTTTGAG GCTGTGGAGG AAGGCCGAAG CTCTAGGCAT
301TCCAAGTCTA GCAGTGACAG GAGCAGAAAA CGAGAGCTAA AGGAGGTGTT TGGTGATGAC
361TCTGAGATCT CTAAAGAATC ATCAGGAGTA AAGAAGCGAC GAATACCCCG TTTTGAGGAG
421GTGGAAGAAG AGCCAGAGGT GATCCCTGGG CCTCCATCAG AGAGCCCTGG CATGCTGACT
481AAGCTCCAGA TCAAACAGAT GATGGAGGCA GCAACACGAC AAATCGAGGA GAGGAAAAAA
541CAGCTGAGCT TCATTAGCCC CCCTACACCT CAGCCAAAGA CTCCTTCTTC CTCCCAACCA
601GAACGACTTC CTATTGGCAA CACTATTCAG CCCTCCCAGG CTGCCACTTT CATGAATGAT
661GCCATTGAGA AGGCAAGGAA AGCAGCTGAA CTGCAAGCTC GAATCCAAGC CCAGCTGGCA
721CTGAAGCCAG GACTCATCGG CAATGCCAAC ATGGTGGGCC TGGCTAATCT CCATGCCATG
781GGCATTGCTC CCCCGAAGGT GGAGTTAAAA GACCAAACGA AACCTACACC ACTGATCCTG
841GATGAGCAAG GGCGCACTGT AGATGCAACA GGCAAGGAGA TTGAGCTGAC ACACCGCATG
901CCTACTCTGA AAGCCAATAT TCGTGCTGTG AAGAGGGAAC AATTCAAGCA ACAACTAAAG
961GAAAAGCCAT CAGAAGACAT GGAATCCAAT ACCTTTTTTG ACCCCCGAGT CTCCATTGCC
1021CCTTCCCAGC GCCAGAGACG CACTTTTAAA TTCCATGACA AGGGCAAATT TGAGAAGATT
1081GCTCAGCGAT TACGGACAAA GGCTCAACTG GAGAAGCTAC AGGCAGAGAT TTCACAAGCA
1141GCTCGAAAAA CAGGCATCCA TACTTCGACT AGGCTTGCCC TCATTGCTCC TAAGAAGGAG
1201CTAAAGGAAG GAGATATTCC TGAAATTGAG TGGTGGGACT CTTACATAAT CCCCAATGGC
1261TTTGATCTTA CAGAGGAAAA TCCCAAGAGA GAAGATTATT TTGGAATCAC AAATCTTGTT
1321GAACATCCAG CCCAGCTCAA TCCTCCAGTT GACAATGACA CACCAGTTAC TCTGGGAGTA
1381TATCTTACCA AGAAGGAACA GAAAAAACTT CGGAGACAAA CAAGGAGGGA AGCACAGAAG
1441GAACTACAAG AAAAAGTCAG GCTGGGCCTG ATGCCTCCTC CAGAACCCAA AGTGAGAATT
1501TCTAATTTGA TGCGAGTATT AGGAACAGAA GCTGTTCAAG ACCCCACGAA GGTAGAAGCC
1561CACGTCAGAG CTCAGATGGC AAAAAGACAG AAAGCGCATG AAGAGGCCAA CGCTGCCCGA
1621AAACTCACAG CAGAACAGAG AAAGGTCAAG AAAATTAAAA AGCTTAAAGA AGACATTTCA
1681CAGGGGGTAC ACATATCTGT ATATAGAGTT CGAAATTTGA GCAACCCAGC CAAGAAGTTC
1741AAGATTGAAG CCAATGCTGG GCAACTGTAC CTGACAGGGG TGGTGGTACT GCACAAGGAT
1801GTCAACGTGG TAGTAGTGGA AGGGGGCCCC AAGGCCCAGA AGAAATTTAA GCGTCTTATG
1861CTGCATCGGA TAAAGTGGGA TGAACAGACA TCTAACACAA AGGGAGATGA TGATGAGGAG
1921TCTGATGAGG AAGCTGTGAA GAAAACCAAC AAATGTGTAC TAGTCTGGGA GGGTACAGCC
1981AAAGACCGGA GCTTTGGAGA GATGAAGTTT AAACAGTGTC CTACAGAGAA CATGGCTCGT
2041GAGCATTTCA AAAAGCATGG GGCTGAACAC TACTGGGACC TTGCGCTGAG TGAATCTGTG
2101TTAGAGTCCA CTGATTGCCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc
2161ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)