Transcript: Human XR_921997.3

PREDICTED: Homo sapiens pre-mRNA processing factor 3 (PRPF3), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PRPF3 (9129)
Length:
2274
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_921997.3
NBCI Gene record:
PRPF3 (9129)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_921997.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000074784 GCGCTGAGTGAATCTGTGTTA pLKO.1 2002 3UTR 100% 4.950 6.930 N PRPF3 n/a
2 TRCN0000286698 GCGCTGAGTGAATCTGTGTTA pLKO_005 2002 3UTR 100% 4.950 6.930 N PRPF3 n/a
3 TRCN0000074787 CGTCTTATGCTGCATCGGATA pLKO.1 1761 3UTR 100% 4.050 5.670 N PRPF3 n/a
4 TRCN0000074786 CCTACTCTGAAAGCCAATATT pLKO.1 929 3UTR 100% 15.000 10.500 N PRPF3 n/a
5 TRCN0000294112 TTCCATGACAAGGGCAAATTT pLKO_005 1079 3UTR 100% 15.000 10.500 N PRPF3 n/a
6 TRCN0000294056 CAGTTACTCTGGGAGTATATC pLKO_005 1392 3UTR 100% 13.200 9.240 N PRPF3 n/a
7 TRCN0000074785 GCCTACTCTGAAAGCCAATAT pLKO.1 928 3UTR 100% 13.200 9.240 N PRPF3 n/a
8 TRCN0000328789 TCCATGACAAGGGCAAATTTG pLKO_005 1080 3UTR 100% 13.200 9.240 N Prpf3 n/a
9 TRCN0000294059 TCTAATTTGATGCGAGTATTA pLKO_005 1529 3UTR 100% 13.200 9.240 N PRPF3 n/a
10 TRCN0000074783 CAGTCCTCTCACTTATTCTAT pLKO.1 2082 3UTR 100% 5.625 3.938 N PRPF3 n/a
11 TRCN0000294057 CACTTGTTTGTGTGATCTCAG pLKO_005 2118 3UTR 100% 4.050 2.835 N PRPF3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_921997.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02088 pDONR223 100% 80.6% None (many diffs) n/a
2 ccsbBroad304_02088 pLX_304 0% 80.6% V5 (many diffs) n/a
3 TRCN0000467879 TATACCCTGATCCCCTGATACGTG pLX_317 17.9% 80.6% V5 (many diffs) n/a
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