Construct: ORF ccsbBroadEn_02827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016740.1_s300c1, BRDN0000456044
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEFF2 (23671)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23671 | TMEFF2 | transmembrane protein with ... | NM_016192.4 | 100% | 100% | |
| 2 | human | 23671 | TMEFF2 | transmembrane protein with ... | XM_011510890.3 | 97.5% | 97.5% | 410_411ins27 |
| 3 | human | 23671 | TMEFF2 | transmembrane protein with ... | NM_001305134.1 | 92.3% | 91.7% | (many diffs) |
| 4 | human | 23671 | TMEFF2 | transmembrane protein with ... | XM_017003739.2 | 89.9% | 89.3% | (many diffs) |
| 5 | human | 23671 | TMEFF2 | transmembrane protein with ... | XM_017003740.2 | 69.7% | 67.3% | (many diffs) |
| 6 | human | 23671 | TMEFF2 | transmembrane protein with ... | NM_001305145.1 | 44.2% | 39.8% | (many diffs) |
| 7 | mouse | 56363 | Tmeff2 | transmembrane protein with ... | NM_019790.4 | 93.4% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1188
- ORF length:
- 1122
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGT GCTGTGGGAG TCCCCGCGGC AGTGCAGCAG CTGGACACTT TGCGAGGGCT 121 TTTGCTGGCT GCTGCTGCTG CCCGTCATGC TACTCATCGT AGCCCGCCCG GTGAAGCTCG 181 CTGCTTTCCC TACCTCCTTA AGTGACTGCC AAACGCCCAC CGGCTGGAAT TGCTCTGGTT 241 ATGATGACAG AGAAAATGAT CTCTTCCTCT GTGACACCAA CACCTGTAAA TTTGATGGGG 301 AATGTTTAAG AATTGGAGAC ACTGTGACTT GCGTCTGTCA GTTCAAGTGC AACAATGACT 361 ATGTGCCTGT GTGTGGCTCC AATGGGGAGA GCTACCAGAA TGAGTGTTAC CTGCGACAGG 421 CTGCATGCAA ACAGCAGAGT GAGATACTTG TGGTGTCAGA AGGATCATGT GCCACAGATG 481 CAGGATCAGG ATCTGGAGAT GGAGTCCATG AAGGCTCTGG AGAAACTAGT CAAAAGGAGA 541 CATCCACCTG TGATATTTGC CAGTTTGGTG CAGAATGTGA CGAAGATGCC GAGGATGTCT 601 GGTGTGTGTG TAATATTGAC TGTTCTCAAA CCAACTTCAA TCCCCTCTGC GCTTCTGATG 661 GGAAATCTTA TGATAATGCA TGCCAAATCA AAGAAGCATC GTGTCAGAAA CAGGAGAAAA 721 TTGAAGTCAT GTCTTTGGGT CGATGTCAAG ATAACACAAC TACAACTACT AAGTCTGAAG 781 ATGGGCATTA TGCAAGAACA GATTATGCAG AGAATGCTAA CAAATTAGAA GAAAGTGCCA 841 GAGAACACCA CATACCTTGT CCGGAACATT ACAATGGCTT CTGCATGCAT GGGAAGTGTG 901 AGCATTCTAT CAATATGCAG GAGCCATCTT GCAGGTGTGA TGCTGGTTAT ACTGGACAAC 961 ACTGTGAAAA AAAGGACTAC AGTGTTCTAT ACGTTGTTCC CGGTCCTGTA CGATTTCAGT 1021 ATGTCTTAAT CGCAGCTGTG ATTGGAACAA TTCAGATTGC TGTCATCTGT GTGGTGGTCC 1081 TCTGCATCAC AAGGAAATGC CCCAGAAGCA ACAGAATTCA CAGACAGAAG CAAAATACAG 1141 GGCACTACAG TTCAGACAAT ACAACAAGAG CGTCCACGAG GTTAATCTAC CCAACTTTCT 1201 TGTACAAAGT TGGcattata agaaagcatt gcttatcaat ttgttgcaac gaac