Construct: ORF ccsbBroadEn_04451

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002638.1_s300c1, BRDN0000393635
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATG4C (84938)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84938 ATG4C autophagy related 4C cystei... NM_032852.4 100% 100%
2 human 84938 ATG4C autophagy related 4C cystei... NM_178221.2 100% 100%
3 human 84938 ATG4C autophagy related 4C cystei... XM_005271288.2 100% 100%
4 human 84938 ATG4C autophagy related 4C cystei... XM_024450414.1 100% 100%
5 human 84938 ATG4C autophagy related 4C cystei... XM_017002600.1 91.2% 91.2% 1087_1088ins120
6 human 84938 ATG4C autophagy related 4C cystei... XM_017002601.2 91.2% 91.2% 1087_1088ins120
7 human 84938 ATG4C autophagy related 4C cystei... XM_011542313.2 89.9% 88.7% (many diffs)
8 human 84938 ATG4C autophagy related 4C cystei... XR_001737493.2 46.4% 1_178del;972_973ins137;1416_2526del
9 human 84938 ATG4C autophagy related 4C cystei... XR_001737492.1 45.8% 1_210del;1004_1005ins137;1448_2558del
10 human 84938 ATG4C autophagy related 4C cystei... XR_001737494.2 41.9% (many diffs)
11 mouse 242557 Atg4c autophagy related 4C, cyste... NM_001145967.1 88% 90.3% (many diffs)
12 mouse 242557 Atg4c autophagy related 4C, cyste... NM_175029.3 88% 90.3% (many diffs)
13 mouse 242557 Atg4c autophagy related 4C, cyste... XM_006503071.3 84.6% 86.8% (many diffs)
14 mouse 242557 Atg4c autophagy related 4C, cyste... XM_006503072.3 77.2% 79.2% (many diffs)
15 mouse 242557 Atg4c autophagy related 4C, cyste... XM_006503073.3 69.3% 70.7% (many diffs)
16 mouse 242557 Atg4c autophagy related 4C, cyste... XR_867864.2 13.6% (many diffs)
17 mouse 242557 Atg4c autophagy related 4C, cyste... XR_867862.2 13.3% (many diffs)
18 mouse 242557 Atg4c autophagy related 4C, cyste... XR_867860.2 13.1% (many diffs)
19 mouse 242557 Atg4c autophagy related 4C, cyste... XR_867865.2 10.7% (many diffs)
20 mouse 242557 Atg4c autophagy related 4C, cyste... XR_376316.3 10.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1440
ORF length:
1374
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA GGCTACAGGA ACAGATGAAG TTGACAAGCT AAAAACCAAA TTTATATCTG
121CTTGGAACAA CATGAAATAT AGTTGGGTGT TGAAAACAAA GACGTATTTT AGTAGAAATT
181CTCCTGTATT ATTGCTTGGA AAATGTTACC ATTTTAAATA TGAAGATGAA GATAAAACGT
241TACCTGCAGA GTCGGGATGT ACAATAGAGG ATCACGTAAT TGCAGGAAAT GTAGAAGAAT
301TTCGTAAAGA TTTCATTTCT AGAATATGGC TGACCTACAG GGAAGAATTC CCTCAAATAG
361AAGGCTCAGC TTTGACAACA GACTGTGGGT GGGGCTGCAC ATTGAGAACT GGCCAGATGC
421TCTTGGCTCA AGGACTCATA CTACACTTTC TTGGTAGAGC TTGGACCTGG CCTGATGCTT
481TGAATATTGA AAATTCAGAC TCTGAATCAT GGACTTCCCA CACTGTCAAA AAATTTACTG
541CATCATTTGA AGCATCACTT TCAGGGGAAA GAGAATTCAA AACCCCAACA ATTTCTCTGA
601AGGAAACAAT TGGGAAATAT TCTGATGATC ATGAAATGCG AAATGAAGTT TATCATAGGA
661AAATCATCTC TTGGTTTGGT GATTCCCCCT TGGCTCTTTT TGGCTTACAT CAACTAATAG
721AATATGGAAA GAAGTCTGGG AAAAAAGCAG GAGATTGGTA TGGACCAGCT GTGGTTGCTC
781ACATTTTAAG AAAAGCAGTT GAAGAAGCAA GGCATCCTGA TTTACAAGGA ATAACTATTT
841ATGTTGCACA AGATTGTACA GTTTACAATT CTGATGTAAT TGATAAACAG AGTGCTTCCA
901TGACTTCTGA TAATGCAGAT GACAAAGCTG TTATTATTCT AGTTCCTGTT AGACTTGGTG
961GAGAAAGAAC CAACACCGAC TACTTAGAAT TTGTGAAGGG TATTTTAAGC CTGGAATATT
1021GTGTGGGTAT TATTGGTGGC AAACCTAAAC AGTCATATTA CTTTGCTGGA TTTCAAGATG
1081ACAGTTTGAT TTACATGGAT CCTCATTACT GCCAATCTTT TGTAGATGTC AGCATAAAGG
1141ATTTCCCTCT TGAGACATTC CACTGCCCTT CTCCCAAAAA GATGTCTTTT CGAAAAATGG
1201ATCCCAGCTG TACAATAGGA TTTTACTGTC GAAATGTTCA GGACTTCAAA CGAGCTTCTG
1261AAGAAATCAC CAAGATGCTG AAATTTTCTT CTAAGGAGAA ATATCCCTTA TTTACTTTTG
1321TAAATGGTCA TTCCAGAGAC TATGATTTTA CATCTACTAC AACCAATGAA GAAGACCTTT
1381TTTCAGAGGA TGAAAAGAAA CAATTAAAAA GATTTAGCAC GGAAGAGTTT GTCTTGCTTT
1441ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
1501acgaac

Download FASTA (ORF) (Full)