Construct: ORF ccsbBroadEn_05198

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012349.1_s300c1, BRDN0000387055
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HACD2 (201562)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 201562 HACD2 3-hydroxyacyl-CoA dehydrata... NM_198402.5 100% 100%
2 human 201562 HACD2 3-hydroxyacyl-CoA dehydrata... NM_001329783.2 91.6% 91.6% 274_342del
3 human 201562 HACD2 3-hydroxyacyl-CoA dehydrata... XR_001740056.2 79.1% 1_52del;735_875del;949_950insTTTGAA
4 human 201562 HACD2 3-hydroxyacyl-CoA dehydrata... NM_001329784.2 63.1% 61.8% (many diffs)
5 human 201562 HACD2 3-hydroxyacyl-CoA dehydrata... XM_024453387.1 63.1% 61.8% (many diffs)
6 human 201562 HACD2 3-hydroxyacyl-CoA dehydrata... NM_001329786.2 56.2% 56.2% 0_1ins333
7 human 201562 HACD2 3-hydroxyacyl-CoA dehydrata... NM_001329787.1 56.2% 56.2% 0_1ins333
8 human 201562 HACD2 3-hydroxyacyl-CoA dehydrata... XM_017005856.2 51% 50.3% (many diffs)
9 mouse 70757 Hacd2 3-hydroxyacyl-CoA dehydrata... NM_023587.2 90.1% 94.8% (many diffs)
10 mouse 70757 Hacd2 3-hydroxyacyl-CoA dehydrata... XM_006522570.3 50.7% 54.3% (many diffs)
11 mouse 70757 Hacd2 3-hydroxyacyl-CoA dehydrata... XM_006522571.1 50.7% 54.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
828
ORF length:
762
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGCAGTGGCG GCGACTGCAG CAGCGAAGGG GAATGGGGGC GGCGGTGGCA
121GGGCCGGGGC CGGGGACGCC AGCGGCACGC GGAAGAAGAA GGGCCCGGGG CCCCTGGCCA
181CGGCGTACCT GGTCATCTAC AATGTGGTGA TGACAGCCGG GTGGCTGGTT ATAGCGGTTG
241GTCTGGTCCG AGCATACCTG GCTAAGGGTA GCTACCATAG CCTTTATTAT TCAATTGAAA
301AGCCTTTGAA ATTCTTTCAA ACTGGAGCCT TATTGGAGAT TTTACATTGT GCTATAGGAA
361TTGTTCCATC TTCTGTTGTC CTGACTTCTT TCCAGGTGAT GTCAAGAGTT TTTCTAATAT
421GGGCAGTAAC ACATAGCGTC AAAGAGGTAC AGAGTGAAGA CAGTGTCCTC CTGTTTGTTA
481TTGCATGGAC GATCACGGAA ATCATCCGTT ACTCCTTTTA TACATTCAGT CTATTAAACC
541ATCTGCCTTA CCTCATCAAA TGGGCCAGGT ACACACTTTT CATTGTGCTG TACCCAATGG
601GAGTGTCAGG AGAACTGCTC ACAATATATG CAGCTCTGCC CTTTGTCAGA CAAGCTGGCC
661TATATTCCAT CAGTTTACCC AACAAATACA ATTTCTCTTT TGACTACTAT GCATTCCTGA
721TTCTAATAAT GATCTCCTAC ATTCCAATTT TTCCCCAGTT ATACTTCCAC ATGATACACC
781AGAGAAGAAA GATCCTTTCT CATACTGAAG AACACAAGAA ATTTGAATAC CCAACTTTCT
841TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)