Transcript: Human NM_001329783.2

Homo sapiens 3-hydroxyacyl-CoA dehydratase 2 (HACD2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
HACD2 (201562)
Length:
4194
CDS:
27..860

Additional Resources:

NCBI RefSeq record:
NM_001329783.2
NBCI Gene record:
HACD2 (201562)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001329783.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000370969 TGATCTCCTACATTCCAATTT pLKO_005 760 CDS 100% 13.200 18.480 N HACD2 n/a
2 TRCN0000159059 GCTTGCAGTATAGTTATGTTT pLKO.1 1243 3UTR 100% 5.625 7.875 N HACD2 n/a
3 TRCN0000161939 GATCACGGAAATCATCCGTTA pLKO.1 521 CDS 100% 0.405 0.567 N HACD2 n/a
4 TRCN0000166515 CGGAAATCATCCGTTACTCCT pLKO.1 526 CDS 100% 2.640 2.112 N HACD2 n/a
5 TRCN0000370970 AGGAGAACTGCTCACAATATA pLKO_005 638 CDS 100% 15.000 10.500 N HACD2 n/a
6 TRCN0000030088 GCTACCATAGCCTTTATTATT pLKO.1 232 CDS 100% 15.000 10.500 N Hacd2 n/a
7 TRCN0000365688 CAGACAAGCTGGCCTATATTC pLKO_005 677 CDS 100% 13.200 9.240 N HACD2 n/a
8 TRCN0000365689 CTATGCATTCCTGATTCTAAT pLKO_005 737 CDS 100% 13.200 9.240 N HACD2 n/a
9 TRCN0000377614 ACACCCTGGCCAGTTCATTTC pLKO_005 1053 3UTR 100% 10.800 7.560 N HACD2 n/a
10 TRCN0000376663 GCATGGACGATCACGGAAATC pLKO_005 513 CDS 100% 10.800 7.560 N HACD2 n/a
11 TRCN0000030086 GCGTACCTGGTCATCTACAAT pLKO.1 144 CDS 100% 5.625 3.938 N Hacd2 n/a
12 TRCN0000160315 CCAGTTATACTTCCACATGAT pLKO.1 785 CDS 100% 4.950 3.465 N HACD2 n/a
13 TRCN0000160585 CTATAGGAATTGTTCCATCTT pLKO.1 382 CDS 100% 4.950 3.465 N HACD2 n/a
14 TRCN0000030085 CCTGACTTCTTTCCAGGTGAT pLKO.1 410 CDS 100% 4.050 2.835 N Hacd2 n/a
15 TRCN0000159607 GTCTATTAAACCATCTGCCTT pLKO.1 559 CDS 100% 2.640 1.848 N HACD2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001329783.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05198 pDONR223 100% 91.6% 91.6% None 274_342del n/a
2 ccsbBroad304_05198 pLX_304 0% 91.6% 91.6% V5 274_342del n/a
3 TRCN0000466043 TCTCGACATAGAGGAGGGGCTGAC pLX_317 47.3% 91.6% 91.6% V5 274_342del n/a
Download CSV