Construct: ORF ccsbBroadEn_06169
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001051.1_s300c1, BRDN0000391294
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA7 (2045)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 2045 | EPHA7 | EPH receptor A7 | NM_004440.4 | 99.9% | 100% | 981G>A;1341T>C |
2 | human | 2045 | EPHA7 | EPH receptor A7 | XM_005248669.3 | 99.5% | 99.4% | 981G>A;1341T>C;1798_1799insTTAAATTTCCAG |
3 | human | 2045 | EPHA7 | EPH receptor A7 | NM_001288629.2 | 99.4% | 99.4% | 981G>A;1341T>C;1616_1617insAGGTAAAATGTTTGA |
4 | human | 2045 | EPHA7 | EPH receptor A7 | XM_017010365.2 | 99% | 98.9% | (many diffs) |
5 | human | 2045 | EPHA7 | EPH receptor A7 | XM_005248671.3 | 60.7% | 60% | (many diffs) |
6 | human | 2045 | EPHA7 | EPH receptor A7 | XM_017010366.2 | 44.2% | 44.1% | 981G>A;1324_1325insCTCCCTCGCAAGT;1326_1327ins1655 |
7 | human | 2045 | EPHA7 | EPH receptor A7 | XR_001743218.2 | 37.4% | (many diffs) | |
8 | human | 2045 | EPHA7 | EPH receptor A7 | NM_001288630.1 | 27.9% | 27.7% | 832_833insCCT;834_835insGGCCGTGGGTTCTAC;837_838ins2139 |
9 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_010141.4 | 91.2% | 98% | (many diffs) |
10 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_001290434.1 | 90.8% | 97.5% | (many diffs) |
11 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_006537606.3 | 90.7% | 97.5% | (many diffs) |
12 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_006537607.3 | 56.4% | 59.3% | (many diffs) |
13 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_001122889.1 | 55.6% | 58.7% | (many diffs) |
14 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_017319954.1 | 55.1% | 58.2% | (many diffs) |
15 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_017319955.1 | 48.6% | 51.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3063
- ORF length:
- 2994
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 tNNGCACCAT GGTTTTTCAA ACTCGGTACC CTTCATGGAT TATTTTATGC TACATCTGGC 121 TGCTCCGCTT TGCACACACA GGGGAGGCGC AGGCTGCGAA GGAAGTACTA CTGCTGGATT 181 CTAAAGCACA ACAAACAGAG TTGGAGTGGA TTTCCTCTCC ACCCAATGGG TGGGAAGAAA 241 TTAGTGGTTT GGATGAGAAC TATACCCCGA TACGAACATA CCAGGTGTGC CAAGTCATGG 301 AGCCCAACCA AAACAACTGG CTGCGGACTA ACTGGATTTC CAAAGGCAAT GCACAAAGGA 361 TTTTTGTAGA ATTGAAATTC ACCCTGAGGG ATTGTAACAG TCTTCCTGGA GTACTGGGAA 421 CTTGCAAGGA AACATTTAAT TTGTACTATT ATGAAACAGA CTATGACACT GGCAGGAATA 481 TAAGAGAAAA CCTCTATGTA AAAATAGACA CCATTGCTGC AGATGAAAGT TTTACCCAAG 541 GTGACCTTGG TGAAAGAAAG ATGAAGCTTA ACACTGAGGT GAGAGAGATT GGACCTTTGT 601 CCAAAAAGGG ATTCTATCTT GCCTTTCAGG ATGTAGGGGC TTGCATAGCT TTGGTTTCTG 661 TCAAAGTGTA CTACAAGAAG TGCTGGTCCA TTATTGAGAA CTTAGCTATC TTTCCAGATA 721 CAGTGACTGG TTCAGAATTT TCCTCTTTAG TCGAGGTTCG AGGGACATGT GTCAGCAGTG 781 CAGAGGAAGA AGCGGAAAAC GCCCCCAGGA TGCACTGCAG TGCAGAAGGA GAATGGTTAG 841 TGCCCATTGG AAAATGTATC TGCAAAGCAG GCTACCAGCA AAAAGGAGAC ACTTGTGAAC 901 CCTGTGGCCG TGGGTTCTAC AAGTCTTCCT CTCAAGATCT TCAGTGCTCT CGTTGTCCAA 961 CTCACAGTTT TTCTGATAAA GAAGGCTCCT CCAGATGTGA ATGTGAAGAT GGGTATTACA 1021 GGGCTCCATC TGACCCACCA TACGTTGCAT GCACAAGGCC TCCATCTGCA CCACAGAACC 1081 TCATTTTCAA CATCAACCAA ACCACAGTAA GTTTGGAATG GAGTCCTCCT GCAGACAATG 1141 GGGGAAGAAA CGATGTGACC TACAGAATAT TGTGTAAGCG GTGCAGTTGG GAGCAGGGCG 1201 AATGTGTTCC CTGTGGGAGT AACATTGGAT ACATGCCCCA GCAGACTGGA TTAGAGGATA 1261 ACTATGTCAC TGTCATGGAC CTGCTAGCCC ACGCTAATTA TACTTTTGAA GTTGAAGCTG 1321 TAAATGGAGT TTCTGACTTA AGCCGATCCC AGAGGCTCTT TGCTGCTGTC AGTATCACCA 1381 CTGGTCAAGC AGCTCCCTCG CAAGTGAGCG GAGTAATGAA GGAGAGAGTA CTGCAGCGGA 1441 GTGTCGAGCT TTCCTGGCAG GAACCAGAGC ATCCCAATGG AGTCATCACA GAATATGAAA 1501 TCAAGTATTA CGAGAAAGAT CAAAGGGAAC GGACCTACTC AACAGTAAAA ACCAAGTCTA 1561 CTTCAGCCTC CATTAATAAT CTGAAACCAG GAACAGTGTA TGTTTTCCAG ATTCGGGCTT 1621 TTACTGCTGC TGGTTATGGA AATTACAGTC CCAGACTTGA TGTTGCTACA CTAGAGGAAG 1681 CTACAGGTAA AATGTTTGAA GCTACAGCTG TCTCCAGTGA ACAGAATCCT GTTATTATCA 1741 TTGCTGTGGT TGCTGTAGCT GGGACCATCA TTTTGGTGTT CATGGTCTTT GGCTTCATCA 1801 TTGGGAGAAG GCACTGTGGT TATAGCAAAG CTGACCAAGA AGGCGATGAA GAGCTTTACT 1861 TTCATTTTAA ATTTCCAGGC ACCAAAACCT ACATTGACCC TGAAACCTAT GAGGACCCAA 1921 ATAGAGCTGT CCATCAATTC GCCAAGGAGC TAGATGCCTC CTGTATTAAA ATTGAGCGTG 1981 TGATTGGTGC AGGAGAATTC GGTGAAGTCT GCAGTGGCCG TTTGAAACTT CCAGGGAAAA 2041 GAGATGTTGC AGTAGCCATA AAAACCCTGA AAGTTGGTTA CACAGAAAAA CAAAGGAGAG 2101 ACTTTTTGTG TGAAGCAAGC ATCATGGGGC AGTTTGACCA CCCGAATGTT GTCCATTTGG 2161 AAGGGGTTGT TACAAGAGGG AAACCAGTCA TGATAGTAAT AGAGTTCATG GAAAATGGAG 2221 CCCTAGATGC ATTTCTCAGG AAACATGATG GGCAATTTAC AGTCATTCAG TTAGTAGGAA 2281 TGCTGAGAGG AATTGCTGCT GGAATGAGAT ATTTGGCTGA TATGGGATAT GTTCACAGGG 2341 ACCTTGCAGC TCGCAATATT CTTGTCAACA GCAATCTCGT TTGTAAAGTG TCAGATTTTG 2401 GCCTGTCCCG AGTTATAGAG GATGATCCAG AAGCTGTCTA TACAACTACT GGTGGAAAAA 2461 TTCCAGTAAG GTGGACAGCA CCCGAAGCCA TCCAGTACCG GAAATTCACA TCAGCCAGTG 2521 ATGTATGGAG CTATGGAATA GTCATGTGGG AAGTTATGTC TTATGGAGAA AGACCTTATT 2581 GGGACATGTC AAATCAAGAT GTTATAAAAG CAATAGAAGA AGGTTATCGT TTACCAGCAC 2641 CCATGGACTG CCCAGCTGGC CTTCACCAGC TAATGTTGGA TTGTTGGCAA AAGGAGCGTG 2701 CTGAAAGGCC AAAATTTGAA CAGATAGTTG GAATTCTAGA CAAAATGATT CGAAACCCAA 2761 ATAGTCTGAA AACTCCCCTG GGAACTTGTA GTAGGCCAAT AAGCCCTCTT CTGGATCAAA 2821 ACACTCCTGA TTTCACTACC TTTTGTTCAG TTGGAGAATG GCTACAAGCT ATTAAGATGG 2881 AAAGATATAA AGATAATTTC ACGGCAGCTG GCTACAATTC CCTTGAATCA GTAGCCAGGA 2941 TGACTATTGA GGATGTGATG AGTTTAGGGA TCACACTGGT TGGTCATCAA AAGAAAATCA 3001 TGAGCAGCAT TCAGACTATG AGAGCACAAA TGCTACATTT ACATGGAACT GGCATTCAAG 3061 TGTTGCCAAC TTTTTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt 3121 gcaacgaac