Construct: ORF ccsbBroadEn_06863

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000543.1_s300c1, BRDN0000396560
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RGS7 (6000)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6000 RGS7 regulator of G protein sign... NM_002924.6 99.9% 99.7% 1227G>T
2 human 6000 RGS7 regulator of G protein sign... NM_001282778.2 96.2% 96% 1227G>T;1358_1359ins54
3 human 6000 RGS7 regulator of G protein sign... NM_001364886.1 95.5% 95.1% (many diffs)
4 human 6000 RGS7 regulator of G protein sign... NM_001282775.2 94.6% 93.8% (many diffs)
5 human 6000 RGS7 regulator of G protein sign... XM_017002004.2 92.7% 92.6% 174_175ins51;1176G>T;1307_1308ins54
6 human 6000 RGS7 regulator of G protein sign... XM_017002001.2 92.1% 91.7% (many diffs)
7 human 6000 RGS7 regulator of G protein sign... XM_017002003.2 91.2% 90.3% (many diffs)
8 human 6000 RGS7 regulator of G protein sign... NM_001350115.1 90.8% 90.7% 0_1ins78;1149G>T;1280_1281ins54
9 human 6000 RGS7 regulator of G protein sign... NM_001350116.1 90.8% 90.7% 0_1ins78;1149G>T;1280_1281ins54
10 human 6000 RGS7 regulator of G protein sign... XM_017002009.1 90.8% 90.7% 0_1ins78;1149G>T;1280_1281ins54
11 human 6000 RGS7 regulator of G protein sign... XM_006711800.4 90.3% 89.9% (many diffs)
12 human 6000 RGS7 regulator of G protein sign... XM_011544246.3 90.3% 89.9% (many diffs)
13 human 6000 RGS7 regulator of G protein sign... XM_011544247.3 90.3% 89.9% (many diffs)
14 human 6000 RGS7 regulator of G protein sign... XM_017002002.2 90.3% 89.9% (many diffs)
15 human 6000 RGS7 regulator of G protein sign... NM_001350113.1 89.3% 88.5% (many diffs)
16 human 6000 RGS7 regulator of G protein sign... NM_001350114.1 89.3% 88.5% (many diffs)
17 human 6000 RGS7 regulator of G protein sign... XM_017002005.2 89.3% 88.5% (many diffs)
18 human 6000 RGS7 regulator of G protein sign... NM_001282773.2 83.8% 82.9% (many diffs)
19 human 6000 RGS7 regulator of G protein sign... XM_017002013.1 78.9% 78.8% 0_1ins252;975G>T;1106_1107ins54
20 human 6000 RGS7 regulator of G protein sign... XM_017002011.2 78.7% 78.3% (many diffs)
21 human 6000 RGS7 regulator of G protein sign... XM_017002012.2 77.6% 76.5% (many diffs)
22 mouse 24012 Rgs7 regulator of G protein sign... XM_006496830.4 90.8% 97.9% (many diffs)
23 mouse 24012 Rgs7 regulator of G protein sign... XM_011238808.3 88.3% 94.9% (many diffs)
24 mouse 24012 Rgs7 regulator of G protein sign... XM_030253948.1 87.4% 94.4% (many diffs)
25 mouse 24012 Rgs7 regulator of G protein sign... NM_011880.3 87.3% 94.4% (many diffs)
26 mouse 24012 Rgs7 regulator of G protein sign... XM_006496828.4 87% 93.9% (many diffs)
27 mouse 24012 Rgs7 regulator of G protein sign... XM_006496829.4 87% 93.9% (many diffs)
28 mouse 24012 Rgs7 regulator of G protein sign... NM_001199003.1 85.9% 91.6% (many diffs)
29 mouse 24012 Rgs7 regulator of G protein sign... NM_001347195.1 85.9% 92.6% (many diffs)
30 mouse 24012 Rgs7 regulator of G protein sign... XM_017320465.2 83.9% 90.9% (many diffs)
31 mouse 24012 Rgs7 regulator of G protein sign... XM_006496831.4 83.8% 90.5% (many diffs)
32 mouse 24012 Rgs7 regulator of G protein sign... XM_030253949.1 82.6% 88.1% (many diffs)
33 mouse 24012 Rgs7 regulator of G protein sign... XM_006496834.4 77.2% 83.3% (many diffs)
34 mouse 24012 Rgs7 regulator of G protein sign... XM_017320468.2 77.2% 83.5% (many diffs)
35 mouse 24012 Rgs7 regulator of G protein sign... XM_011238809.3 75.9% 81.7% (many diffs)
36 mouse 24012 Rgs7 regulator of G protein sign... NM_001357129.1 75.9% 80.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1527
ORF length:
1461
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC CCAGGGGAAT AATTATGGGC AGACCAGCAA CGGGGTGGCC GATGAATCAC
121CCAACATGCT GGTGTACAGA AAGATGGAAG ACGTCATAGC ACGGATGCAA GATGAAAAAA
181ATGGAATTCC TATTCGTACG GTCAAAAGCT TTCTTTCCAA GATACCTAGC GTCTTCTCTG
241GTTCAGACAT TGTTCAATGG TTGATAAAGA ACTTAACTAT AGAAGATCCA GTGGAGGCGC
301TCCATTTGGG AACATTAATG GCTGCCCACG GCTACTTCTT TCCAATCTCA GATCATGTCC
361TCACACTCAA GGATGATGGC ACCTTTTACC GGTTTCAAAC CCCCTATTTT TGGCCATCAA
421ATTGTTGGGA GCCGGAAAAC ACAGATTATG CCGTTTACCT CTGCAAGAGA ACAATGCAAA
481ACAAGGCACG ACTGGAGCTC GCAGACTATG AGGCTGAGAG CCTGGCCAGG CTGCAGAGAG
541CATTTGCCCG GAAGTGGGAG TTCATTTTCA TGCAAGCAGA AGCACAAGCA AAAGTGGACA
601AGAAGAGAGA CAAGATTGAA AGGAAGATCC TTGACAGCCA AGAGAGAGCG TTCTGGGACG
661TGCACAGGCC CGTGCCTGGA TGTGTAAATA CAACTGAAGT GGACATTAAG AAGTCATCCA
721GAATGAGAAA CCCCCACAAA ACACGGAAGT CTGTCTATGG TTTACAAAAT GATATTAGAA
781GTCACAGTCC TACCCACACA CCCACACCAG AAACTAAACC TCCAACAGAA GATGAGTTAC
841AACAACAGAT AAAATATTGG CAAATACAGT TAGATAGACA TCGGTTAAAA ATGTCAAAAG
901TCGCTGACAG TCTACTAAGT TACACGGAAC AGTATTTAGA ATACGACCCG TTTCTTTTGC
961CACCTGACCC TTCTAACCCA TGGCTGTCCG ATGACACCAC TTTCTGGGAA CTTGAGGCAA
1021GCAAAGAACC GAGCCAGCAG AGGGTAAAAC GATGGGGTTT TGGCATGGAC GAGGCATTGA
1081AAGACCCAGT TGGGAGAGAA CAGTTCCTTA AATTTCTAGA GTCAGAATTC AGCTCGGAAA
1141ATTTAAGATT CTGGCTGGCA GTGGAGGACC TGAAAAAGAG GCCTATTAAA GAAGTACCCT
1201CAAGAGTTCA GGAAATATGG CAAGAGTTTC TGGCTCCCGG AGCCCCCAGT GCTATTAACT
1261TGGATTCCAA GAGTTATGAC AAAACCACAC ATAACGTGAA GGAACCTGGA CGATACACAT
1321TTGAAGATGC TCAGGAGCAC ATTTACAAAC TGATGAAAAG TGATTCATAC CCACGTTTTA
1381TAAGATCCAG TGCCTATCAG GAGCTTCTAC AGGCAAAGAA AAAGTCTGGA AACTCAATGG
1441ATCGCAGAAC ATCTTTTGAA AAATTTGCAC AGAATGTGGG GAAATCTCTC ACGTCCAAGA
1501GGTTAACAAG CCTTGCTCAG TCTTACTACC CAACTTTCTT GTACAAAGTt ggcattataa
1561gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)