Construct: ORF ccsbBroadEn_07263

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017449.1_s300c1, BRDN0000456892
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPF18 (8559)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8559 PRPF18 pre-mRNA processing factor 18 NM_003675.4 99.8% 99.7% 8T>C;549A>G
2 human 8559 PRPF18 pre-mRNA processing factor 18 XM_011519754.3 94.9% 93.8% (many diffs)
3 human 8559 PRPF18 pre-mRNA processing factor 18 XM_017016860.2 86% 85.9% 8T>C;549A>G;578_579ins141
4 human 8559 PRPF18 pre-mRNA processing factor 18 XR_002957038.1 24.6% (many diffs)
5 human 8559 PRPF18 pre-mRNA processing factor 18 XR_001747251.2 10.5% (many diffs)
6 human 8559 PRPF18 pre-mRNA processing factor 18 XR_001747250.1 9.9% (many diffs)
7 human 8559 PRPF18 pre-mRNA processing factor 18 XR_002957037.1 7.5% (many diffs)
8 human 8559 PRPF18 pre-mRNA processing factor 18 XR_001747249.1 6.5% (many diffs)
9 mouse 67229 Prpf18 pre-mRNA processing factor 18 NM_026045.3 89.9% 98.8% (many diffs)
10 mouse 67229 Prpf18 pre-mRNA processing factor 18 XM_006497530.3 87.6% 96.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1092
ORF length:
1026
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG CACAAAAAAG
61TTGGCATGGA CACTCTGAAA TCAGAGATCC TTCGGAAGCG GCAGCTGGTG GAGGACAGGA
121ACCTGCTGGT GGAAAATAAA AAATATTTCA AGCGTAGTGA GCTCGCCAAA AAAGAAGAGG
181AAGCATATTT TGAAAGATGT GGCTACAAGA TACAGCCAAA AGAGGAGGAC CAGAAACCAT
241TAACTTCATC GAATCCAGTG TTAGAACTTG AACTGGCAGA GGAAAAATTA CCTATGACGC
301TTTCTAGGCA AGAGGTCATC AGAAGATTGA GAGAAAGAGG AGAACCAATC AGACTATTTG
361GAGAGACTGA TTATGATGCT TTTCAACGTT TAAGGAAAAT AGAGATCCTC ACACCAGAAG
421TTAACAAGGG ATTGAGGAAT GATTTGAAAG CAGCCTTGGA TAAGATTGAT CAGCAGTACC
481TCAATGAAAT CGTCGGCGGT CAGGAGCCTG GAGAGGAAGA CACACAGAAT GATCTGAAAG
541TTCATGAGGA AAACACCACA ATTGAAGAGT TAGAGGCGCT TGGAGAGTCC TTAGGGAAAG
601GCGATGATCA TAAGGACATG GACATCATCA CCAAATTCCT GAAGTTTCTT CTTGGCGTTT
661GGGCTAAAGA ATTGAATGCC AGAGAAGATT ATGTGAAACG CAGTGTGCAG GGTAAACTGA
721ACAGTGCGAC CCAGAAACAG ACCGAGTCCT ACCTAAGACC ACTTTTTAGA AAGCTACGGA
781AAAGGAATCT TCCTGCTGAT ATTAAAGAAT CAATAACGGA TATTATTAAA TTCATGTTGC
841AGAGAGAATA CGTGAAGGCA AATGATGCTT ATCTTCAGAT GGCCATTGGA AATGCGCCTT
901GGCCCATCGG TGTCACTATG GTTGGTATCC ATGCCAGAAC TGGCAGAGAA AAGATTTTTT
961CCAAGCATGT TGCACATGTT TTAAATGACG AAACTCAGCG GAAATATATT CAGGGATTGA
1021AGAGGTTAAT GACCATTTGC CAGAAACACT TTCCTACAGA CCCATCCAAA TGTGTGGAGT
1081ACAATGCACT GTGCCCAACT TTCTTGTACA AAGTTGGcat tataagaaag cattgcttat
1141caatttgttg caacgaac

Download FASTA (ORF) (Full)

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