Transcript: Human XR_002957037.1

PREDICTED: Homo sapiens pre-mRNA processing factor 18 (PRPF18), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PRPF18 (8559)
Length:
13483
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002957037.1
NBCI Gene record:
PRPF18 (8559)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002957037.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000010512 GACGAAACTCAGCGGAAATAT pLKO.1 1198 3UTR 100% 15.000 12.000 N PRPF18 n/a
2 TRCN0000314724 GACGAAACTCAGCGGAAATAT pLKO_005 1198 3UTR 100% 15.000 12.000 N PRPF18 n/a
3 TRCN0000000016 GATCTGTGTATGGTGTGTTAA pLKO.1 1306 3UTR 100% 13.200 9.240 N PRPF18 n/a
4 TRCN0000314726 GATCTGTGTATGGTGTGTTAA pLKO_005 1306 3UTR 100% 13.200 9.240 N PRPF18 n/a
5 TRCN0000000019 GAATACGTGAAGGCAAATGAT pLKO.1 1057 3UTR 100% 5.625 3.938 N PRPF18 n/a
6 TRCN0000000018 GACTATTTGGAGAGACTGATT pLKO.1 563 3UTR 100% 4.950 3.465 N PRPF18 n/a
7 TRCN0000000017 GAGGAGAACCAATCAGACTAT pLKO.1 548 3UTR 100% 4.950 3.465 N PRPF18 n/a
8 TRCN0000314723 GAGGAGAACCAATCAGACTAT pLKO_005 548 3UTR 100% 4.950 3.465 N PRPF18 n/a
9 TRCN0000314652 GGAATCTTCCTGCTGATATTA pLKO_005 995 3UTR 100% 15.000 9.000 N PRPF18 n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3044 3UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000180418 GATCACTTGAGCTCAGGAGTT pLKO.1 7163 3UTR 100% 4.050 2.025 Y ERN2 n/a
12 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3045 3UTR 100% 13.200 6.600 Y LIAS n/a
13 TRCN0000148774 CCATGTGTTCTCATTGTTCAA pLKO.1 6063 3UTR 100% 4.950 2.475 Y GLIPR1L2 n/a
14 TRCN0000162188 CCATGTGTTCTCATTGTTCAA pLKO.1 6063 3UTR 100% 4.950 2.475 Y SPC25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002957037.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07263 pDONR223 100% 7.5% None (many diffs) n/a
2 ccsbBroad304_07263 pLX_304 0% 7.5% V5 (many diffs) n/a
3 TRCN0000480692 TTCAGCATCACCTTTAACGAACCA pLX_317 42.2% 7.5% V5 (many diffs) n/a
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