Construct: ORF ccsbBroadEn_07799

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008490.1_s300c1, BRDN0000392794
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
U2AF2 (11338)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11338 U2AF2 U2 small nuclear RNA auxili... NM_001012478.1 99.9% 100% 1407C>T
2 human 11338 U2AF2 U2 small nuclear RNA auxili... NM_007279.2 99% 99.1% 1032_1043del;1419C>T
3 human 11338 U2AF2 U2 small nuclear RNA auxili... XM_011526410.1 77.1% 77.2% (many diffs)
4 human 11338 U2AF2 U2 small nuclear RNA auxili... XM_006722994.2 64.4% 64.6% (many diffs)
5 mouse 22185 U2af2 U2 small nuclear ribonucleo... NM_133671.3 91.2% 100% (many diffs)
6 mouse 22185 U2af2 U2 small nuclear ribonucleo... NM_001205231.1 90.4% 99.1% (many diffs)
7 mouse 22185 U2af2 U2 small nuclear ribonucleo... XM_006539728.3 88.1% 96.8% (many diffs)
8 mouse 22185 U2af2 U2 small nuclear ribonucleo... XM_006539727.3 87.3% 96% (many diffs)
9 mouse 22185 U2af2 U2 small nuclear ribonucleo... XM_017322124.1 70.4% 77.2% (many diffs)
10 mouse 22185 U2af2 U2 small nuclear ribonucleo... XM_006539730.2 58.8% 64.6% (many diffs)
11 mouse 102640468 LOC102640468 splicing factor U2AF 65 kDa... XR_001782861.1 30.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1479
ORF length:
1413
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC GGACTTCGAC GAGTTCGAGC GGCAGCTCAA CGAGAATAAA CAAGAGCGGG
121ACAAGGAGAA CCGGCATCGG AAGCGCAGCC ACAGCCGCTC TCGGAGCCGG GACCGCAAAC
181GCCGGAGCCG GAGCCGCGAC CGGCGCAACC GGGACCAGCG GAGCGCCTCC CGGGACAGGC
241GACGACGCAG CAAACCTTTG ACCAGAGGCG CTAAAGAGGA GCACGGTGGA CTGATTCGTT
301CCCCCCGCCA CGAGAAGAAG AAGAAGGTCC GTAAATACTG GGACGTGCCA CCCCCAGGCT
361TTGAGCACAT CACCCCAATG CAGTACAAGG CCATGCAAGC TGCGGGTCAG ATTCCAGCCA
421CTGCTCTTCT CCCCACCATG ACCCCTGACG GTCTGGCTGT GACCCCAACG CCGGTGCCCG
481TGGTCGGGAG CCAGATGACC AGACAAGCCC GGCGCCTCTA CGTGGGCAAC ATCCCCTTTG
541GCATCACTGA GGAGGCCATG ATGGATTTCT TCAACGCCCA GATGCGCCTG GGGGGGCTGA
601CCCAGGCCCC TGGCAACCCA GTGTTGGCTG TGCAGATTAA CCAGGACAAG AATTTTGCCT
661TTTTGGAGTT CCGCTCAGTG GACGAGACTA CCCAGGCTAT GGCCTTTGAT GGCATCATCT
721TCCAGGGCCA GTCACTAAAG ATCCGCAGGC CTCACGACTA CCAGCCGCTT CCTGGCATGT
781CAGAGAACCC CTCCGTCTAT GTGCCTGGGG TTGTGTCCAC TGTGGTCCCC GACTCTGCCC
841ACAAGCTGTT CATCGGGGGC TTACCCAACT ACCTGAACGA TGACCAGGTC AAAGAGCTGC
901TGACATCCTT TGGGCCCCTC AAGGCCTTCA ACCTGGTCAA GGACAGTGCC ACGGGGCTCT
961CCAAGGGCTA CGCCTTCTGT GAGTACGTGG ACATCAACGT CACGGATCAG GCCATTGCGG
1021GGCTGAACGG CATGCAGCTG GGGGATAAGA AGCTGCTGGT CCAGAGGGCG AGTGTGGGAG
1081CCAAGAATGC CACGCTGAGC ACCATCAATC AGACGCCTGT GACCCTGCAA GTGCCGGGCT
1141TGATGAGCTC CCAGGTGCAG ATGGGCGGCC ACCCGACTGA GGTCCTGTGC CTCATGAACA
1201TGGTGCTGCC TGAGGAGCTG CTGGACGACG AGGAGTATGA GGAGATCGTG GAGGATGTGC
1261GGGACGAGTG CAGCAAGTAC GGGCTTGTCA AGTCCATCGA GATCCCCCGG CCTGTGGACG
1321GCGTCGAGGT GCCCGGCTGC GGAAAGATCT TTGTGGAGTT CACCTCTGTG TTTGACTGCC
1381AGAAAGCCAT GCAGGGCCTG ACGGGCCGCA AGTTCGCCAA CAGAGTGGTT GTCACAAAAT
1441ACTGTGACCC CGACTCTTAT CACCGCCGGG ATTTCTGGTA CCCAACTTTC TTGTACAAAG
1501Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)