Construct: ORF ccsbBroadEn_08799

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017962.1_s300c1, BRDN0000398217
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HPSE2 (60495)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 60495 HPSE2 heparanase 2 (inactive) NM_021828.4 99.8% 99.6% 1540G>A;1736A>T
2 human 60495 HPSE2 heparanase 2 (inactive) XM_017016495.1 91.7% 90.8% (many diffs)
3 human 60495 HPSE2 heparanase 2 (inactive) NM_001166246.1 91.7% 90.7% (many diffs)
4 human 60495 HPSE2 heparanase 2 (inactive) XM_011540029.1 91.6% 91.5% 1463_1464ins147;1589A>T
5 human 60495 HPSE2 heparanase 2 (inactive) XM_011540030.1 90.7% 90.5% 447_448ins162;1378G>A;1574A>T
6 human 60495 HPSE2 heparanase 2 (inactive) NM_001166244.1 90% 89.8% 609_610ins174;1366G>A;1562A>T
7 human 60495 HPSE2 heparanase 2 (inactive) NM_001166245.1 80.9% 80.7% 445_446ins336;1204G>A;1400A>T
8 human 60495 HPSE2 heparanase 2 (inactive) XM_017016496.2 71% 59.3% (many diffs)
9 human 60495 HPSE2 heparanase 2 (inactive) XM_006717937.2 70.8% 70.6% 0_1ins516;1024G>A;1220A>T
10 human 60495 HPSE2 heparanase 2 (inactive) XM_011540031.2 70.8% 70.6% 0_1ins516;1024G>A;1220A>T
11 human 60495 HPSE2 heparanase 2 (inactive) XM_017016497.1 70.8% 70.6% 0_1ins516;1024G>A;1220A>T
12 human 60495 HPSE2 heparanase 2 (inactive) XM_024448119.1 70.8% 70.6% 0_1ins516;1024G>A;1220A>T
13 human 60495 HPSE2 heparanase 2 (inactive) XM_017016498.1 54.6% 54.3% 0_1ins804;736G>A;932A>T
14 human 60495 HPSE2 heparanase 2 (inactive) XM_024448120.1 54.6% 54.3% 0_1ins804;736G>A;932A>T
15 human 60495 HPSE2 heparanase 2 (inactive) XR_001747170.1 40.7% (many diffs)
16 mouse 545291 Hpse2 heparanase 2 NM_001081257.2 93% 97.1% (many diffs)
17 mouse 545291 Hpse2 heparanase 2 XM_006527214.3 83.3% 87.3% (many diffs)
18 mouse 545291 Hpse2 heparanase 2 XM_011247294.2 71.1% 72% (many diffs)
19 mouse 545291 Hpse2 heparanase 2 XM_011247295.1 65.7% 69.7% (many diffs)
20 mouse 545291 Hpse2 heparanase 2 XM_011247296.2 53% 53.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1845
ORF length:
1776
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GAGGGTGCTT TGTGCCTTCC CTGAAGCCAT GCCCTCCAGC AACTCCCGCC
121CCCCCGCGTG CCTAGCCCCG GGGGCTCTCT ACTTGGCTCT GTTGCTCCAT CTCTCCCTTT
181CCTCCCAGGC TGGAGACAGG AGACCCTTGC CTGTAGACAG AGCTGCAGGT TTGAAGGAAA
241AGACCCTGAT TCTACTTGAT GTGAGCACCA AGAACCCAGT CAGGACAGTC AATGAGAACT
301TCCTCTCTCT GCAGCTGGAT CCGTCCATCA TTCATGATGG CTGGCTCGAT TTCCTAAGCT
361CCAAGCGCTT GGTGACCCTG GCCCGGGGAC TTTCGCCCGC CTTTCTGCGC TTCGGGGGCA
421AAAGGACCGA CTTCCTGCAG TTCCAGAACC TGAGGAACCC GGCGAAAAGC CGCGGGGGCC
481CGGGCCCGGA TTACTATCTC AAAAACTATG AGGATGACAT TGTTCGAAGT GATGTTGCCT
541TAGATAAACA GAAAGGCTGC AAGATTGCCC AGCACCCTGA TGTTATGCTG GAGCTCCAAA
601GGGAGAAGGC AGCTCAGATG CATCTGGTTC TTCTAAAGGA GCAATTCTCC AATACTTACA
661GTAATCTCAT ATTAACAGCC AGGTCTCTAG ACAAACTTTA TAACTTTGCT GATTGCTCTG
721GACTCCACCT GATATTTGCT CTAAATGCAC TGCGTCGTAA TCCCAATAAC TCCTGGAACA
781GTTCTAGTGC CCTGAGTCTG TTGAAGTACA GCGCCAGCAA AAAGTACAAC ATTTCTTGGG
841AACTGGGTAA TGAGCCAAAT AACTATCGGA CCATGCATGG CCGGGCAGTA AATGGCAGCC
901AGTTGGGAAA GGATTACATC CAGCTGAAGA GCCTGTTGCA GCCCATCCGG ATTTATTCCA
961GAGCCAGCTT ATATGGCCCT AATATTGGGC GGCCGAGGAA GAATGTCATC GCCCTCCTAG
1021ATGGATTCAT GAAGGTGGCA GGAAGTACAG TAGATGCAGT TACCTGGCAA CATTGCTACA
1081TTGATGGCCG GGTGGTCAAG GTGATGGACT TCCTGAAAAC TCGCCTGTTA GACACACTCT
1141CTGACCAGAT TAGGAAAATT CAGAAAGTGG TTAATACATA CACTCCAGGA AAGAAGATTT
1201GGCTTGAAGG TGTGGTGACC ACCTCAGCTG GAGGCACAAA CAATCTATCC GATTCCTATG
1261CTGCAGGATT CTTATGGTTG AACACTTTAG GAATGCTGGC CAATCAGGGC ATTGATGTCG
1321TGATACGGCA CTCATTTTTT GACCATGGAT ACAATCACCT CGTGGACCAG AATTTTAACC
1381CATTACCAGA CTACTGGCTC TCTCTCCTCT ACAAGCGCCT GATCGGCCCC AAAGTCTTGG
1441CTGTGCATGT GGCTGGGCTC CAGCGGAAGC CACGGCCTGG CCGAGTGATC CGGGACAAAC
1501TAAGGATTTA TGCTCACTGC ACAAACCACC ACAACCACAA CTACGTTCGT GGGTCCATTA
1561CACTTTTTAT CATCAACTTG CATCGATCAA GAAAGAAAAT CAAGCTGACT GGGACTCTCA
1621GAGACAAGCT GGTTCACCAG TACCTGCTGC AGCCCTATGG GCAGGAGGGC CTAAAGTCCA
1681AGTCAGTGCA ACTGAATGGC CAGCCCTTAG TGATGGTGGA CGACGGGACC CTCCCAGAAT
1741TGAAGCCCCG CCCCCTTCGG GCCGGCCGGA CATTGGTCAT CCCTCCAGTC ACCATGGGCT
1801TTTTTGTGGT CAAGAATGTC AATGCTTTGG CCTGCCGCTA CCGATTGCCA ACTTTCTTGT
1861ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)