Construct: ORF ccsbBroadEn_12587

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF017002.1_s300c1, BRDN0000387559
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: ICE2 (79664)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	79664	ICE2	interactor of little elonga...	NM_001018089.3	39.2%	39.2%	1_1539del;1932T>C
2	human	79664	ICE2	interactor of little elonga...	XM_005254662.2	39.2%	39.2%	1_1539del;1932T>C
3	human	79664	ICE2	interactor of little elonga...	XM_011522010.2	39.2%	39.2%	1_1539del;1932T>C
4	human	79664	ICE2	interactor of little elonga...	XM_011522011.2	39.2%	39.2%	1_1539del;1932T>C
5	human	79664	ICE2	interactor of little elonga...	XM_024450051.1	39.2%	39.2%	1_1539del;1932T>C
6	human	79664	ICE2	interactor of little elonga...	NM_024611.6	33.7%	33.8%	1_1950del;2343T>C
7	human	79664	ICE2	interactor of little elonga...	XM_005254661.2	33.7%	33.8%	1_1950del;2343T>C
8	human	79664	ICE2	interactor of little elonga...	XM_011522009.2	33.7%	33.8%	1_1950del;2343T>C
9	human	79664	ICE2	interactor of little elonga...	XM_017022569.2	33.7%	33.8%	1_1950del;2343T>C
10	human	79664	ICE2	interactor of little elonga...	XR_931903.3	30.2%		(many diffs)
11	human	79664	ICE2	interactor of little elonga...	XR_931902.3	25.8%		(many diffs)
12	human	79664	ICE2	interactor of little elonga...	XR_001751386.2	25.5%		(many diffs)
13	human	79664	ICE2	interactor of little elonga...	NR_147171.2	13.9%		(many diffs)
14	mouse	93697	Ice2	interactor of little elonga...	XM_006511575.3	33.3%	33.6%	(many diffs)
15	mouse	93697	Ice2	interactor of little elonga...	XM_006511574.3	29.9%	30.2%	(many diffs)
16	mouse	93697	Ice2	interactor of little elonga...	NM_145618.3	28.9%	29.1%	(many diffs)
17	mouse	93697	Ice2	interactor of little elonga...	XM_006511573.3	28.9%	29.1%	(many diffs)
18	mouse	93697	Ice2	interactor of little elonga...	XR_379471.3	16.8%		(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1062

ORF length:

996

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61	TTGGCATGCA GGATGAGCTC TTAAAGCCAA TTTCCAGAAA AGTACCAGAA TTGCCCTTAA
121	TGAATTTAGA AAATTCTAAA CAGCCTTCTG TTTCTGAGCA ATTGTCTGGT CCTTCAGACT
181	CCTCTAGTTG GCCGAAATCT GGATGGCCTT CTGCATTTCA GAAGCCAAAA GGACGATTGC
241	CATATGAACT TCAGGACTAT GTTGAAGATA CATCGGAATA CCTAGCTCCT CAGGAAGGAA
301	ATTTTGTTTA TAAGTTATTT AGCCTGCAAG ACCTGTTGTT ACTCGTACGC TGCAGTGTCC
361	AGAGGATAGA GACAAGACCA CGTTCTAAAA AACGGAAGAA AATCAGAAGA CAATTTCCAG
421	TTTATGTACT ACCAAAAGTA GAGTATCAAG CTTGTTACGG AGTTGAAGCT CTGACTGAAA
481	GTGAACTTTG TCGCTTATGG ACTGAAAGTT TATTGCATTC CAACAGCTCA TTTTATGTTG
541	GGCATATCGA TGCATTTACT TCAAAACTTT TTCTACTGGA AGAAATTACC TCAGAAGAAT
601	TAAAAGAAAA GCTTTCAGCA CTCAAGATTT CCAATTTATT TAACATCCTC CAACACATTC
661	TAAAGAAACT AAGTAGCTTG CAGGAGGGTT CCTACTTGTT ATCTCATGCA GCAGAAGATT
721	CTTCACTCCT GATTTATAAG GCCTCTGATG GAAAAGTTAC TAGGACAGCA TACAATTTGT
781	ATAAAACACA TTGCGGCCTT CCTGGTGTAC CTTCCAGTCT CTCAGTTCCC TGGGTCCCAT
841	TAGATCCCAG CCTGTTATTA CCATATCATA TCCATCATGG AAGAATACCT TGTACTTTTC
901	CACCGAAATC ACTGGATACC ACAACACAAC AAAAGATTGG TGGAACGAGA ATGCCTACAC
961	GCAGCCACAG GAATCCAGTT TCCATGGAAA CCAAAAGCAG TTGCTTGCCT GCTCAGCAAG
1021	TTGAAACTGA AGGAGTGGCT CCACATAAAA GAAAAATAAC TTGCCCAACT TTCTTGTACA
1081	AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

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