Construct: ORF ccsbBroadEn_13166

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003083.1_s300c1, BRDN0000390174
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
METTL6 (131965)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 131965 METTL6 methyltransferase like 6 NM_152396.3 89.7% 89.7% 766_852del
2 human 131965 METTL6 methyltransferase like 6 XM_005264867.4 89.7% 89.7% 766_852del
3 human 131965 METTL6 methyltransferase like 6 XM_006712970.4 89.7% 89.7% 766_852del
4 human 131965 METTL6 methyltransferase like 6 XM_017005718.2 89.7% 89.7% 766_852del
5 human 131965 METTL6 methyltransferase like 6 XM_011533357.3 78.4% 64.5% (many diffs)
6 human 131965 METTL6 methyltransferase like 6 NM_001301791.1 78.4% 65.9% (many diffs)
7 human 131965 METTL6 methyltransferase like 6 XM_006712972.4 78.3% 72.6% (many diffs)
8 human 131965 METTL6 methyltransferase like 6 NM_001330662.2 77.9% 69.1% (many diffs)
9 human 131965 METTL6 methyltransferase like 6 NM_001301792.1 75.2% 67.5% 530_531ins142;576_577ins47
10 human 131965 METTL6 methyltransferase like 6 XM_017005722.2 75.2% 67.5% 530_531ins142;576_577ins47
11 human 131965 METTL6 methyltransferase like 6 NM_001301790.1 73.9% 73.9% 223_224ins135;631_717del
12 human 131965 METTL6 methyltransferase like 6 XM_017005719.1 73.9% 73.9% 223_224ins135;631_717del
13 human 131965 METTL6 methyltransferase like 6 XM_017005724.2 71.7% 70.5% (many diffs)
14 human 131965 METTL6 methyltransferase like 6 XM_017005723.1 61.5% 55.3% (many diffs)
15 human 131965 METTL6 methyltransferase like 6 XR_002959491.1 40.9% 1_246del;776_1613del;1850_1867del
16 human 131965 METTL6 methyltransferase like 6 XR_001740019.2 18.6% 1_246del;775_860del;1098_4111del
17 human 131965 METTL6 methyltransferase like 6 XR_001740018.2 16.6% (many diffs)
18 human 131965 METTL6 methyltransferase like 6 XR_940377.3 12% 1_246del;775_860del;1098_6351del
19 mouse 67011 Mettl6 methyltransferase like 6 NM_025907.3 78.8% 80.1% (many diffs)
20 mouse 67011 Mettl6 methyltransferase like 6 XM_006519432.3 78.8% 80.1% (many diffs)
21 mouse 67011 Mettl6 methyltransferase like 6 XM_006519434.2 78.7% 75.9% (many diffs)
22 mouse 67011 Mettl6 methyltransferase like 6 XM_011245154.1 78.6% 78% (many diffs)
23 mouse 67011 Mettl6 methyltransferase like 6 XM_011245155.1 76.3% 77.6% (many diffs)
24 mouse 67011 Mettl6 methyltransferase like 6 XM_017316161.1 67.1% 68.2% (many diffs)
25 mouse 67011 Mettl6 methyltransferase like 6 XM_006519436.3 65.2% 66.6% (many diffs)
26 mouse 67011 Mettl6 methyltransferase like 6 XM_006519437.3 64.8% 58.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
831
ORF length:
765
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC TTCTTTGCAA AGGAAAGGGC TGCAGGCAAG GATTCTCACC TCTGAAGAAG
121AGGAGAAACT GAAAAGAGAC CAAACTTTGG TGTCTGATTT TAAACAGCAG AAATTGGAAC
181AAGAGGCTCA GAAAAATTGG GATCTTTTTT ACAAAAGAAA TAGCACTAAT TTCTTCAAAG
241ACAGACACTG GACCACCAGA GAGTTTGAGG AGCTAAGATC ATGTAGAGAG TTTGAAGATC
301AAAAGTTAAC AATGCTTGAA GCTGGCTGTG GGGTTGGAAA CTGTTTATTC CCACTTTTAG
361AAGAAGATCC GAATATCTTT GCCTATGCCT GTGATTTTTC TCCAAGAGCC ATTGAATATG
421TTAAGCAAAA TCCTTTATAT GATACAGAAA GATGCAAGGT ATTCCAGTGT GATCTGACTA
481AAGATGATCT TCTGGATCAT GTACCGCCAG AGTCTGTGGA TGTTGTTATG TTGATATTTG
541TGCTGTCAGC TGTTCATCCT GATAAGATGC ACCTTGTCTT ACAAAACATT TACAAGGTAT
601TAAAACCAGG CAAAAGTGTC TTGTTTCGTG ACTACGGACT GTATGATCAT GCCATGCTTA
661GGTTTAAAGC CAGCAGCAAA CTTGGAGAAA ACTTTTATGT TAGACAAGAT GGGACCAGAT
721CATATTTTTT TACTGATGAC TTCCTGGCTC AGCTCTTTAT GGACACAGGT TATGAAGAAG
781TGGTAAACGA GTATGTGTTT CGAGAGACGG TGAATAAAAA AGAAGGCCTG TGCCCAACTT
841TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)