Construct: ORF ccsbBroadEn_13848

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007939.1_s300c1, BRDN0000457298
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EGFR (1956)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1956 EGFR epidermal growth factor rec... NM_001346897.2 99.8% 99.8% (many diffs)
2 human 1956 EGFR epidermal growth factor rec... NM_001346898.2 95.9% 95.9% (many diffs)
3 human 1956 EGFR epidermal growth factor rec... NM_001346899.1 92.2% 89.7% (many diffs)
4 human 1956 EGFR epidermal growth factor rec... NM_005228.5 88.8% 86.5% (many diffs)
5 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2239_2247del 88.6% 86.2% (many diffs)
6 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2240_2254del 88.5% 86.1% (many diffs)
7 human 1956 EGFR epidermal growth factor rec... NM_001346900.2 84.5% 82.1% (many diffs)
8 human 1956 EGFR epidermal growth factor rec... NM_001346941.2 73.3% 70.8% (many diffs)
9 human 1956 EGFR epidermal growth factor rec... NM_201284.2 56.2% 50.1% (many diffs)
10 human 1956 EGFR epidermal growth factor rec... NM_201282.2 51.2% 51.2% (many diffs)
11 human 1956 EGFR epidermal growth factor rec... NM_201283.1 31.6% 31.5% (many diffs)
12 mouse 13649 Egfr epidermal growth factor rec... NM_207655.2 76.3% 79.1% (many diffs)
13 mouse 13649 Egfr epidermal growth factor rec... NM_007912.4 44.8% 46.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3342
ORF length:
3273
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GCGACCCTCC GGGACGGCCG GGGCAGCGCT CCTGGCGCTG CTGGCTGCGC
121TCTGCCCGGC GAGTCGGGCT CTGGAGGAAA AGAAAGTTTG CCAAGGCACG AGTAACAAGC
181TCACGCAGTT GGGCACTTTT GAAGATCATT TTCTCAGCCT CCAGAGGATG TTCAATAACT
241GTGAGGTGGT CCTTGGGAAT TTGGAAATTA CCTATGTGCA GAGGAATTAT GATCTTTCCT
301TCTTAAAGAC CATCCAGGAG GTGGCTGGTT ATGTCCTCAT TGCCCTCAAC ACAGTGGAGC
361GAATTCCTTT GGAAAACCTG CAGATCATCA GAGGAAATAT GTACTACGAA AATTCCTATG
421CCTTAGCAGT CTTATCTAAC TATGATGCAA ATAAAACCGG ACTGAAGGAG CTGCCCATGA
481GAAATTTACA TGGACAAAAG TGTGATCCAA GCTGTCCCAA TGGGAGCTGC TGGGGTGCAG
541GAGAGGAGAA CTGCCAGAAA CTGACCAAAA TCATCTGTGC CCAGCAGTGC TCCGGGCGCT
601GCCGTGGCAA GTCCCCCAGT GACTGCTGCC ACAACCAGTG TGCTGCAGGC TGCACAGGCC
661CCCGGGAGAG CGACTGCCTG GTCTGCCGCA AATTCCGAGA CGAAGCCACG TGCAAGGACA
721CCTGCCCCCC ACTCATGCTC TACAACCCCA CCACGTACCA GATGGATGTG AACCCCGAGG
781GCAAATACAG CTTTGGTGCC ACCTGCGTGA AGAAGTGTCC CCGTAATTAT GTGGTGACAG
841ATCACGGCTC GTGCGTCCGA GCCTGTGGGG CCGACAGCTA TGAGATGGAG GAAGACGGCG
901TCCGCAAGTG TAAGAAGTGC GAAGGGCCTT GCCGCAAAGT GTGTAACGGA ATAGGTATTG
961GTGAATTTAA AGACTCACTC TCCATAAATG CTACGAATAT TAAACACTTC AAAAACTGCA
1021CCTCCATCAG TGGCGATCTC CACATCCTGC CGGTGGCATT TAGGGGTGAC TCCTTCACAC
1081ATACTCCTCC TCTGGATCCA CAGGAACTGG ATATTCTGAA AACCGTAAAG GAAATCACAG
1141GGTTTTTGCT GATTCAGGCT TGGCCTGAAA ACAGGACGGA CCTCCATGCC TTTGAGAACC
1201TAGAAATCAT ACGCGGCAGG ACCAAGCAAC ATGGTCAGTT TTCTCTTGCA GTCGTCAGCC
1261TGAACATAAC ATCCTTGGGA TTACGCTCCC TCAAGGAGAT AAGTGATGGA GATGTGATAA
1321TTTCAGGAAA CAAAAATTTG TGCTATGCAA ATACAATAAA CTGGAAAAAA CTGTTTGGGA
1381CCTCCGGTCA GAAAACCAAA ATTATAAGCA ACAGAGGTGA AAACAGCTGC AAGGCCACAG
1441GCCAGGTCTG CCATGCCTTG TGCTCCCCCG AGGGCTGCTG GGGCCCGGAG CCCAGGGACT
1501GCGTCTCTTG CCGGAATGTC AGCCGAGGCA GGGAATGCGT GGACAAGTGC AACCTTCTGG
1561AGGGTGAGCC AAGGGAGTTT GTGGAGAACT CTGAGTGCAT ACAGTGCCAC CCAGAGTGCC
1621TGCCTCAGGC CATGAACATC ACCTGCACAG GACGGGGACC AGACAACTGT ATCCAGTGTG
1681CCCACTACAT TGACGGCCCC CACTGCGTCA AGACCTGCCC GGCAGGAGTC ATGGGAGAAA
1741ACAACACCCT GGTCTGGAAG TACGCAGACG CCGGCCATGT GTGCCACCTG TGCCATCCAA
1801ACTGCACCTA CGGATGCACT GGGCCAGGTC TTGAAGGCTG TCCAACGAAT GGGCCTAAGA
1861TCCCGTCCAT CGCCACTGGG ATGGTGGGGG CCCTCCTCTT GCTGCTGGTG GTGGCCCTGG
1921GGATCGGCCT CTTCATGCGA AGGCGCCACA TCGTTCGGAA GCGCACGCTG CGGAGGCTGC
1981TGCAGGAGAG GGAGCTTGTG GAGCCTCTTA CACCCAGTGG AGAAGCTCCC AACCAAGCTC
2041TCTTGAGGAT CTTGAAGGAA ACTGAATTCA AAAAGATCAA AGTGCTGGGC TCCGGTGCGT
2101TCGGCACGGT GTATAAGGGA CTCTGGATCC CAGAAGGTGA GAAAGTTAAA ATTCCCGTCG
2161CTATCAAGGA ATTAAGAGAA GCAACATCTC CGAAAGCCAA CAAGGAAATC CTCGATGAAG
2221CCTACGTGAT GGCCAGCGTG GACAACCCCC ACGTGTGCCG CCTGCTGGGC ATCTGCCTCA
2281CCTCCACCGT GCAGCTCATC ACGCAGCTCA TGCCCTTCGG CTGCCTCCTG GACTATGTCC
2341GGGAACACAA AGACAATATT GGCTCCCAGT ACCTGCTCAA CTGGTGTGTG CAGATCGCAA
2401AGGGCATGAA CTACTTGGAG GACCGTCGCT TGGTGCACCG CGACCTGGCA GCCAGGAACG
2461TACTGGTGAA AACACCGCAG CATGTCAAGA TCACAGATTT TGGGCTGGCC AAACTGCTGG
2521GTGCGGAAGA GAAAGAATAC CATGCAGAAG GAGGCAAAGT GCCTATCAAG TGGATGGCAT
2581TGGAATCAAT TTTACACAGA ATCTATACCC ACCAGAGTGA TGTCTGGAGC TACGGGGTGA
2641CCGTTTGGGA GTTGATGACC TTTGGATCCA AGCCATATGA CGGAATCCCT GCCAGCGAGA
2701TCTCCTCCAT CCTGGAGAAA GGAGAACGCC TCCCTCAGCC ACCCATATGT ACCATCGATG
2761TCTACATGAT CATGGTCAAG TGCTGGATGA TAGACGCAGA TAGTCGCCCA AAGTTCCGTG
2821AGTTGATCAT CGAATTCTCC AAAATGGCCC GAGACCCCCA GCGCTACCTT GTCATTCAGG
2881GGGATGAAAG AATGCATTTG CCAAGTCCTA CAGACTCCAA CTTCTACCGT GCCCTGATGG
2941ATGAAGAAGA CATGGACGAC GTGGTGGATG CCGACGAGTA CCTCATCCCA CAGCAGGGCT
3001TCTTCAGCAG CCCCTCCACG TCACGGACTC CCCTCCTGAG CTCTCTGAGT GCAACCAGCA
3061ACAATTCCAC CGTGGCTTGC ATTGATAGAA ATGGGCTGCA AAGCTGTCCC ATCAAGGAAG
3121ACAGCTTCTT GCAGCGATAC AGCTCAGACC CCACAGGCGC CTTGACTGAG GACAGCATAG
3181ACGACACCTT CCTCCCAGTG CCTGGTGAGT GGCTTGTCTG GAAACAGTCC TGCTCCTCAA
3241CCTCCTCGAC CCACTCAGCA GCAGCCAGTC TCCAGTGTCC AAGCCAGGTG CTCCCTCCAG
3301CATCTCCAGA GGGGGAAACA GTGGCAGATT TCCAGACACA GTTGCCAACT TTCTTGTACA
3361AAGTTGGcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)