Construct: ORF ccsbBroadEn_13887
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011708.1_s300c1, BRDN0000457065
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF5C (3800)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3800 | KIF5C | kinesin family member 5C | XM_011511157.2 | 99.9% | 99.8% | 1321G>T |
| 2 | human | 3800 | KIF5C | kinesin family member 5C | NM_004522.3 | 75.1% | 75.1% | (many diffs) |
| 3 | human | 3800 | KIF5C | kinesin family member 5C | XM_017004062.1 | 75.1% | 75.1% | (many diffs) |
| 4 | human | 3800 | KIF5C | kinesin family member 5C | NR_111932.2 | 35.3% | 1_68del;1389G>T;2244_6142del | |
| 5 | mouse | 16574 | Kif5c | kinesin family member 5C | NM_008449.2 | 67.1% | 73.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2244
- ORF length:
- 2175
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGCCACTTAC ATCCACGTCA GCAAAACTGG TGCCGAGGGA GCTGTTCTTG 121 ACGAAGCTAA AAATATCAAT AAGTCTTTGT CTGCTCTTGG AAATGTGATC TCTGCTTTGG 181 CAGAAGGGAC AAAAACACAT GTGCCATACC GGGACAGCAA GATGACTCGG ATTCTTCAGG 241 ACTCTTTGGG TGGGAACTGC AGAACCACCA TCGTCATTTG CTGTTCTCCT TCTGTCTTCA 301 ATGAGGCTGA GACCAAGTCC ACACTGATGT TCGGACAGAG AGCTAAGACC ATCAAGAATA 361 CAGTCTCTGT GAACCTAGAA CTGACAGCAG AAGAATGGAA GAAGAAATAT GAAAAAGAGA 421 AAGAGAAAAA CAAGACTTTG AAGAATGTTA TCCAGCATCT GGAGATGGAG CTAAACAGGT 481 GGAGGAATGG AGAAGCTGTG CCTGAGGATG AACAGATCAG TGCCAAGGAC CAGAAGAACC 541 TGGAGCCTTG TGATAACACC CCCATCATAG ACAATATTGC TCCTGTTGTT GCTGGCATCT 601 CTACAGAGGA GAAAGAGAAG TACGATGAGG AGATCTCCAG TCTCTACAGA CAACTGGATG 661 ACAAGGATGA TGAAATTAAC CAGCAGAGCC AGCTGGCTGA AAAGCTGAAG CAACAGATGT 721 TGGATCAGGA TGAGCTTTTA GCTTCCACAA GAAGAGACTA TGAGAAGATA CAGGAGGAGC 781 TGACACGTCT CCAGATTGAA AATGAGGCAG CCAAGGATGA GGTGAAAGAA GTTCTCCAGG 841 CCCTGGAGGA GCTGGCTGTC AATTATGACC AGAAATCACA GGAAGTGGAG GATAAGACCC 901 GGGCCAATGA GCAGCTGACA GACGAGCTGG CCCAGAAAAC GACTACATTG ACAACCACAC 961 AGAGAGAGCT GAGCCAGCTA CAAGAGCTTA GCAACCACCA GAAGAAAAGG GCAACTGAGA 1021 TCCTGAATTT GCTGTTGAAA GATCTGGGGG AGATAGGTGG AATTATTGGC ACCAATGATG 1081 TGAAAACTTT GGCAGATGTG AATGGAGTCA TTGAGGAGGA GTTTACCATG GCCCGCCTGT 1141 ACATCAGCAA GATGAAGTCA GAGGTCAAGT CCCTGGTGAA CCGCAGCAAA CAGCTCGAGA 1201 GCGCCCAGAT GGACTCCAAC AGGAAGATGA ATGCCAGCGA GCGGGAGCTG GCAGCCTGCC 1261 AGCTGCTCAT CTCCCAGCAC GAAGCCAAGA TCAAGTCTCT GACAGACTAC ATGCAGAACA 1321 TGGAACAGAA GAGGAGGCAG CTAGAAGAGT CCCAGGACTC GCTCAGCGAA GAGCTGGCAA 1381 AGCTCCGATC CCAGGAAAAA ATGCACGAAG TCAGCTTCCA GGATAAGGAG AAGGAACATC 1441 TGACGCGGTT GCAGGATGCT GAAGAAATGA AGAAGGCGCT GGAGCAGCAG ATGGAGAGCC 1501 ACCGGGAAGC TCACCAGAAG CAGCTGTCCA GACTCCGAGA CGAAATTGAG GAGAAGCAGA 1561 AAATCATTGA TGAGATTCGG GATTTGAATC AGAAACTGCA ACTGGAACAG GAGAAGCTTA 1621 GTTCTGATTA TAACAAGCTG AAAATAGAGG ACCAAGAGAG AGAAATGAAG CTGGAAAAGC 1681 TCTTATTGCT CAACGATAAA AGGGAACAAG CCAGAGAAGA CCTCAAAGGG CTGGAGGAGA 1741 CAGTGTCTAG AGAATTGCAG ACACTGCACA ACCTTCGGAA ACTCTTTGTC CAGGATCTGA 1801 CCACCCGAGT TAAAAAAAGT GTGGAGTTGG ACAACGATGA TGGAGGGGGC AGTGCTGCCC 1861 AGAAGCAGAA AATTTCCTTC TTGGAGAATA ACCTGGAGCA GCTCACCAAA GTTCACAAGC 1921 AGCTGGTCCG GGACAACGCA GACCTGCGCT GTGAACTGCC CAAGCTGGAG AAGCGGCTGC 1981 GTGCCACGGC GGAGCGCGTC AAGGCTCTGG AGAGCGCGCT GAAGGAGGCC AAGGAGAACG 2041 CCATGCGGGA CCGTAAGCGC TACCAGCAGG AGGTGGATCG TATCAAGGAG GCCGTGCGGG 2101 CCAAGAACAT GGCCAGAAGG GCCCATTCAG CCCAGATCGC CAAGCCCATC CGCCCCGGAC 2161 ACTACCCGGC CTCATCTCCA ACGGCCGTCC ATGCCATTCG AGGGGGAGGA GGCAGCTCTT 2221 CAAATTCCAC TCACTACCAG AAATTGCCAA CTTTCTTGTA CAAAGTTGGc attataagaa 2281 agcattgctt atcaatttgt tgcaacgaac