Construct: ORF ccsbBroadEn_14919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018901.1_s300c1, BRDN0000458197
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRPF4B (8899)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NM_003913.5 | 99.5% | 25.9% | (many diffs) |
| 2 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011410.2 | 99.5% | 25.9% | (many diffs) |
| 3 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011411.2 | 99.5% | 25.9% | (many diffs) |
| 4 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011413.2 | 99.5% | 25.9% | (many diffs) |
| 5 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011414.2 | 99.5% | 25.9% | (many diffs) |
| 6 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011415.2 | 99.5% | 25.9% | (many diffs) |
| 7 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011416.2 | 99.5% | 25.9% | (many diffs) |
| 8 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011417.2 | 99.5% | 25.9% | (many diffs) |
| 9 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011418.2 | 99.5% | 25.9% | (many diffs) |
| 10 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011420.2 | 99.5% | 25.9% | (many diffs) |
| 11 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011421.2 | 99.5% | 25.9% | (many diffs) |
| 12 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011422.2 | 99.5% | 25.9% | (many diffs) |
| 13 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011423.2 | 99.5% | 25.9% | (many diffs) |
| 14 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011424.2 | 99.5% | 25.9% | (many diffs) |
| 15 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011425.2 | 99.5% | 25.9% | (many diffs) |
| 16 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011426.2 | 99.5% | 25.9% | (many diffs) |
| 17 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011427.2 | 99.5% | 25.9% | (many diffs) |
| 18 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011428.2 | 99.5% | 25.9% | (many diffs) |
| 19 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011430.2 | 99.5% | 25.9% | (many diffs) |
| 20 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011432.2 | 85.2% | 30.1% | (many diffs) |
| 21 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011433.2 | 85.2% | 30.1% | (many diffs) |
| 22 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011434.2 | 85.2% | 30.1% | (many diffs) |
| 23 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011435.2 | 85.2% | 30.1% | (many diffs) |
| 24 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146784.2 | 83.9% | (many diffs) | |
| 25 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_011514970.3 | 81.4% | 31.4% | (many diffs) |
| 26 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146783.2 | 80.9% | (many diffs) | |
| 27 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146785.2 | 79.3% | (many diffs) | |
| 28 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | NM_013830.2 | 88.6% | 23.1% | (many diffs) |
| 29 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315425.1 | 88.6% | 23.1% | (many diffs) |
| 30 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315426.1 | 88.6% | 23.1% | (many diffs) |
| 31 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315427.1 | 88.6% | 23.1% | (many diffs) |
| 32 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315428.1 | 88.6% | 23.1% | (many diffs) |
| 33 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315429.1 | 88.6% | 23.1% | (many diffs) |
| 34 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315430.1 | 88.6% | 23.1% | (many diffs) |
| 35 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315431.1 | 88.6% | 23.1% | (many diffs) |
| 36 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315432.1 | 88.6% | 23.1% | (many diffs) |
| 37 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315433.1 | 88.6% | 23.1% | (many diffs) |
| 38 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315434.1 | 88.6% | 23.1% | (many diffs) |
| 39 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315435.1 | 88.6% | 23.1% | (many diffs) |
| 40 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315436.1 | 88.6% | 23.1% | (many diffs) |
| 41 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315437.1 | 88.6% | 23.1% | (many diffs) |
| 42 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_006516582.3 | 77.2% | 26% | (many diffs) |
| 43 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_006516583.3 | 77.1% | 26% | (many diffs) |
| 44 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_006516584.3 | 76.8% | 26.2% | (many diffs) |
| 45 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315438.1 | 76.8% | 26.2% | (many diffs) |
| 46 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315439.1 | 56.1% | 33.2% | (many diffs) |
| 47 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315440.1 | 56.1% | 33.2% | (many diffs) |
| 48 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315441.1 | 54.7% | 37.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 888
- ORF length:
- 789
- Sequence:
-
1 ACCGGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GGCCGCCGCG GAGACCCAGT 121 CGCTACGGGA GCAGCCAGAG ATGGAAGATG CTAATTCTGA AAAGAGTATA AATGAAGAAA 181 ATGGAGAAGT ATCAGAAGAC CAGTCTCAAA ATAAGCACAG TCGTCACAAA AAAAAGAAGC 241 ATAAACACAG AAGTAAACAT AAGAAACATA AACATTCCTC AGAAGAAGAC AAGGATAAAA 301 AACATAAACA TAAGCATAAA CATAAGAAAC ACAAAAGAAA AGAGGTTATT GATGCTTCTG 361 ATAAAGAGGG TATGTCTCCA GCAAAAAGAA CTAAACTTGA TGATTTAGCT TTGCTAGAAG 421 ACTTGGAAAA ACAGAGAGCC TTGATTAAGG CCGAACTTGA TAATGAGTTA ATGGAAGGAA 481 AGGTCCAGTC TGGTATGGGG CTCATTTTGC AAGGTTATGA GTCTGGCTCT GAAGAAGAGG 541 GGGAAATTCA TGAAAAGGCA AGAAATGGAA ATAGGTCTAG TACTAGATCT TCAAGTACAA 601 AGGGGAAACT TGAACTTGTG GACAATAAAA TTACTACAAA GAAACGAAGT AAAAGCAGAT 661 CCAAAGAACG GACTAGACAT AGGTCTGATA AAAAGAAAAG TAAGGGGGGT ATTGAAATCG 721 TTAAAGAGAA AACAACTAGG AGCAAGTCAA AGGAGAGGAA AAAATCTAAA AGCCCATCCA 781 AAAGAAGTAA GTCTCAAGAT CAAGCAAGGA AATCAAAATC CCCTACCCTT AGAAGGCGAT 841 CTCAAGAGAA AATTGGTAAG GCCAGATCTC CTACTGATGA TAAGGGTTAA AATTgaagat 901 aaaagtaaat caaaagatag gaaaaaatcc ccaattataa atgaaagtag aagtcgcgat 961 cgaggtaaaa aatccagatc cccagttgat ttaagaggta aatccaaaga cagaaggtca 1021 cggtccaaag agagaaaatc aaaacggtct gaaactgata aagaaaagaa gccaattaaa 1081 tctccctcta aagatgcttc atctgggaaa gaaaataggt cacccagcag aagacctggt 1141 cgtagtccta aaagaagaag tttgtctcca aaaccacgtg ataaatcaag aagaagcagg 1201 tctccacttt tgaatgatag aagatctaag cagagcaaat ccccctcgcg gacactgtct 1261 cctgggagaa gagccaagag ccgatcctta gaaagaaaac gacgagaacc agagaggaga 1321 cgactttctt ctccaagaac acgacctcga gatgatatcc tcagtagacg tgaaagatca 1381 aaagatgcca gccccatcaa tagatggtct ccaacccgaa gaagaagtag atctcccatt 1441 agaaggaggt ctcgttcccc actcagacgt agcaggtctc caagaagaag aagcagatct 1501 actcggagaa gggacagagg tcggaggagc agatcacgct tgcgaaggcg gtctcgatca 1561 cgcggtggtc gtagacgaag gagcagaagc aaagtaaagg aagataaatt taaaggaagt 1621 ctttctgaag gaatgaaagt tgagcaggaa tcttcgtctg atgataacct tgaagacttt 1681 gatgtagagg aagaagatga agaagcccta atagaacaga gaagaatcca aaggcaggca 1741 attgttcaga aatataaata ccttgctgaa gatagcaaca tgtctgtgcc atctgaacca 1801 agcagccccc agagcagtac gagaacacga tcaccatctc cagatgacat tctggagcga 1861 gtagctgctg atgttaaaga gtatgaacgg gaaaatgttg atacatttga ggcctcagtg 1921 aaagccaagc ataatctaat gacagttgaa cagaataatg gttcatctca gaagaagttg 1981 ttggcacctg atatgtttac agaatctgat gatatgtttg ctgcgtattt tgatagtgct 2041 cgtcttcggg ccgctggcat tggaaaagat ttcaaagaga atcccaacct cagagataac 2101 tggaccgatg cagaaggcta ttatcgtgtg aacataggtg aagtcctaga taaacgttac 2161 aatgtgtatg gctacactgg gcaaggtgta ttcagtaatg ttgtacgagc cagagataat 2221 gcaagagcca accaagaagt ggctgtaaag atcatcagaa acaatgagct catgcaaaag 2281 actggtttaa aagaattaga gttcttgaaa aaacttaatg atgctgatcc tgatgacaaa 2341 ATTCATTGTC TGAGATCTTC AGGCACTCTA TCACCAGCAG CATCTTGTCT GGTATTCGAG 2401 CCTCTCAGCA TGAACTTACG AGAGGTGTAA AAAAATATGT NAAAGATGTT GGTCTTCATA 2461 TTAAGNCTGT AAGATCCTAT AGTCAGCAGT TGTTCCTGGC ATTGAAACTC CTTAAAAGAT 2521 GCAATATCNT ACATGCAGAT ATCAAGCCAG ACAATATCCT GGTTAATGAA TCCAAAACTA 2581 TTTTAAAGCT TTGCGATTTT GGGTCGGCTT CACATGTTGC GGATAATGAC ATAACACCTT 2641 ATCTTGTCAG TAGATTTTAT CGTGCTCCTG AAATCATTAT AGGTAAAAGC TATGACTATG 2701 GTATAGATAT GTGGTCTGTA GGTTGCACCT TATACGAACT CTATACTGGA AAAATTTTAT 2761 TCCCTGGCAA AACCAATAAC CATATGCTGA AGCTTGCAAT GGATCTCAAA GGAAAGATGC 2821 CAAATAAGAT GATTCGAAAA GGTGTGTTCA AAGATCAGCA TTTTGATCAA AATCTCAACT 2881 TCATGTACAT AGAAGTTGAT AAAGTAACAG AGAGGGAGAA AGTTACTGTT ATGAGCACCA 2941 TTAATCCAAC TAAGGACCTG TTGGCTGACT TGATTGGGTG CCAGAGACTT CCTGAAGACC 3001 AACGTAAGAA AGTACACCAG CTAAAGGACT TGTTGGACCA GATTCTGATG TTGGACCCAG 3061 CTAAACGAAT TAGCATCAAC CAGGCCCTAC AGCACGCCTT CATCCAGGAA AAAATTTTGC 3121 CAACTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG 3181 AACAGGTCAC TATCAGTCAA AATAAAATCC ATTATGTGCC CATTCGC