Construct: ORF ccsbBroadEn_15307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018869.1_s300c1, BRDN0000458165
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NEK5 (341676)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 341676 | NEK5 | NIMA related kinase 5 | NM_199289.3 | 95.6% | 36% | (many diffs) |
| 2 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_017020555.1 | 89.7% | 33.7% | (many diffs) |
| 3 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_017020557.1 | 86.8% | 32.6% | (many diffs) |
| 4 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_017020556.1 | 83.3% | 34.5% | (many diffs) |
| 5 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_011535066.2 | 81.6% | 36.5% | (many diffs) |
| 6 | human | 341676 | NEK5 | NIMA related kinase 5 | NM_001365552.1 | 80.6% | 30.6% | (many diffs) |
| 7 | human | 341676 | NEK5 | NIMA related kinase 5 | XR_941575.2 | 74.4% | (many diffs) | |
| 8 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_006719808.4 | 67.9% | 42.6% | (many diffs) |
| 9 | human | 341676 | NEK5 | NIMA related kinase 5 | XR_941574.2 | 64.4% | (many diffs) | |
| 10 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_011535068.2 | 53.8% | 52.7% | (many diffs) |
| 11 | human | 341676 | NEK5 | NIMA related kinase 5 | XR_001749542.1 | 48.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 890
- ORF length:
- 792
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GATAAGTACG ATGTGATTAA 121 GGCCATCGGG CAAGGTGCCT TCGGGAAAGC ATACTTAGCT AAAGGGAAAT CAGATAGCAA 181 GCACTGTGTC ATAAAAGAGA TCAATTTTGA AAAGATGCCC ATACAAGAAA AAGAAGCTTC 241 AAAGAAAGAA GTGATTCTTC TGGAAAAGAT GAAACATCCC AACATTGTAG CCTTCTTCAA 301 TTCATTTCAA GAGAATGGCA GGCTGTTTAT TGTAATGGAA TATTGTGATG GAGGGGATCT 361 CATGAAAAGG ATCAATAGAC AACGGGGTGT GTTATTTAGT GAAGATCAGA TCCTCGGTTG 421 GTTTGTACAG ATTTCTCTAG GACTAAAACA TATTCATGAC AGGAAGATAT TACACAGGGA 481 CATAAAAGCT CAGAACATTT TTCTTAGCAA GAACGGAATG GTGGCAAAGC TTGGGGACTT 541 TGGTATAGCA AGAGTCCTGA ATAATTCCAT GGAACTTGCT CGAACTTGTA TTGGAACACC 601 TTACTACCTG TCCCCAGAGA TCTGTCAGAA TAAACCCTAC AACAATAAAA CGGATATTTG 661 GTCTCTTGGC TGTGTCTTAT ATGAGCTCTG CACACTTAAA CATCCTTTTG AGGGTAACAA 721 CTTACAGCAG CTGGTTCTGA AGATTTGTCA AGCACATTTT GCCCCAATAT CTCCGGGGTT 781 TTCTCGTGAG CTCCATTCCT TGATATCTCA GCTCTTTCAA GTATCTCTCG AGACCGACAT 841 CATAAATTCC ATTTTGAAAG CCCTTTTAGA GAATCTTATT CCCAATATTT GACTCCTGGA 901 GGTCATTCAG GAGGaattca gtcacatgct tatatgcaga gcaggagcgc cagcttctcg 961 acatgctggg aaggtggtcc agaagtgtaa aatacaaaaa gtgagattcc agggaaagtg 1021 cccaccaaga tcaaggatat ctgtgccaat taaaaggaat gctatattgc atagaaatga 1081 atggagacca ccagctggag cccagaaggc cagatctata aaaatgatag aaagacccaa 1141 aattgctgct gtctgtggac attatgatta ttattatgct caacttgata tgctgaggag 1201 gagagcccac aaaccaagtt atcaccctat tcctcaagaa aatactggag ttgaggatta 1261 cggtcaggaa acgaggcatg gtccatcccc aagtcaatgg cctgctgagt accttcagag 1321 aaaatttgaa gGTCCACAAT ATAAGTTTGA AAGTGGAGAA GCAATTGGGT CTTCGTCCAT 1381 CTCTTGCCGA GCCAAATTAC AACCAAGAGA CAAGAGCTAG NAAGTAATGG AGAAGAGCCT 1441 AGATTCCAGG AGCTGCCATT TAGGAAAAAC GAAATGAAGG AACAGGAATA TTGGAAGCAG 1501 TTAGAGGAAA TACGCCAACA GTACCACAAT GACATGAAAG AAATTAGAAA GAAGATGGGG 1561 AGAGAACCAG AGGAGAACTC AAAAATAAGT CATAAAACCT ATTTGGTGAA GAAGAGTAAC 1621 CTGCCTGTCC ATCAAGATGC ATCTGAGGGA GAAGCACCTG TGCAGGACAT TGAAAAAGAC 1681 TTGAAACAAA TGAGGCTTCA GAACACAAAG GAAAGTAAAA ATCCAGAACA GAAATATAAA 1741 GCTAAGAAGG GGGTAAAATT TGAAATTAAT TTAGACAAAT GTATTTCTGA TGAAAACATC 1801 CTCCAAGAGG AAGAGGCAAT GGATATACCA AATGAAACTT TGACCTTTGA GGATGGCATG 1861 AAGTTTAAGG AATATGAATG TGTAAAGGAG CATGGAGATT ATACAGACAA AGCATTTGAA 1921 AAACTTCACT GCCCAGAAGC AGGGTTTTCC ACGCAGACTG TAGCTGCTGT GGGAAACAGG 1981 AGGCAGTGGG ATGGAGGAGC GCCTCAGACT CTGCTGCAGA TGATGGCAGT GGCCGACATC 2041 ACCTCCACCT GCCCCACGGG GCCTGACAGT GAGTCTGTGC TTAGCGTCAG TCGTCAGGAA 2101 GGGAAGACCA AGGACCCGTA CAGCCCAGTG CTCATCCTGA TGTTGCCAAC TTTCTTGTAC 2161 AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC 2221 AGTCAAAATA AAATCATTAT TGCCATCCCA GCCTGATAT