Transcript: Human XM_017020555.1

PREDICTED: Homo sapiens NIMA related kinase 5 (NEK5), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NEK5 (341676)
Length:
2372
CDS:
22..2289

Additional Resources:

NCBI RefSeq record:
XM_017020555.1
NBCI Gene record:
NEK5 (341676)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017020555.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358267 ACAGATTTCTCTAGGACTAAA pLKO_005 567 CDS 100% 13.200 9.240 N NEK5 n/a
2 TRCN0000196614 GCATGAAGTTTAAGGAATATG pLKO.1 2000 CDS 100% 13.200 9.240 N NEK5 n/a
3 TRCN0000358266 CCTTCGGGAAAGCATACTTAG pLKO_005 278 CDS 100% 10.800 7.560 N NEK5 n/a
4 TRCN0000021411 GCCCACCAAGATCAAGGATAT pLKO.1 1166 CDS 100% 10.800 7.560 N NEK5 n/a
5 TRCN0000021412 CCAACAGTACCACAATGACAT pLKO.1 1659 CDS 100% 4.950 3.465 N NEK5 n/a
6 TRCN0000021413 GTAATGGAGAAGAGCCTAGAT pLKO.1 1568 CDS 100% 4.950 3.465 N NEK5 n/a
7 TRCN0000021410 GCCTTCTTCAATTCATTTCAA pLKO.1 430 CDS 100% 5.625 3.375 N NEK5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017020555.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15307 pDONR223 79.8% 89.7% 33.7% None (many diffs) n/a
2 ccsbBroad304_15307 pLX_304 0% 89.7% 33.7% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000473796 AACCCCACCAACCTATGGAACTGT pLX_317 20.5% 89.7% 33.7% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_10033 pDONR223 100% 87.5% 87.4% None 1_216del;233T>C;1785_1786ins75 n/a
5 ccsbBroad304_10033 pLX_304 0% 87.5% 87.4% V5 1_216del;233T>C;1785_1786ins75 n/a
6 TRCN0000479295 TGCCTTTTGATCCACTCGCCGGAC pLX_317 16.5% 87.5% 87.4% V5 1_216del;233T>C;1785_1786ins75 n/a
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