Construct: ORF ccsbBroadEn_15431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018511.1_s300c1, BRDN0000388415
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNAS (2778)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 2778 | GNAS | GNAS complex locus | NM_000516.6 | 99.9% | 100% | 393C>T |
2 | human | 2778 | GNAS | GNAS complex locus | NM_001077488.4 | 99.6% | 99.7% | 257_259delGCA;396C>T |
3 | human | 2778 | GNAS | GNAS complex locus | NM_080426.4 | 96.3% | 96.1% | 212_213insGGGCGGCGAAGAGGACCCG;214_215ins23;351C>T |
4 | human | 2778 | GNAS | GNAS complex locus | NM_001077489.4 | 96.1% | 95.9% | 212_213ins45;348C>T |
5 | human | 2778 | GNAS | GNAS complex locus | XM_024451872.1 | 88.6% | 88.4% | (many diffs) |
6 | human | 2778 | GNAS | GNAS complex locus | NM_001309840.2 | 84.9% | 85% | 0_1ins177;216C>T |
7 | human | 2778 | GNAS | GNAS complex locus | NM_001309861.2 | 84.9% | 85% | 0_1ins177;216C>T |
8 | human | 2778 | GNAS | GNAS complex locus | XM_024451873.1 | 84.9% | 85% | 0_1ins177;216C>T |
9 | human | 2778 | GNAS | GNAS complex locus | XM_024451874.1 | 84.9% | 85% | 0_1ins177;216C>T |
10 | human | 2778 | GNAS | GNAS complex locus | XM_024451875.1 | 84.9% | 85% | 0_1ins177;216C>T |
11 | human | 2778 | GNAS | GNAS complex locus | XM_017027815.1 | 84.8% | 84.3% | (many diffs) |
12 | human | 2778 | GNAS | GNAS complex locus | XM_017027817.1 | 81.1% | 80.9% | 0_1ins177;35_36ins45;171C>T |
13 | human | 2778 | GNAS | GNAS complex locus | XM_017027818.2 | 81.1% | 80.9% | 0_1ins177;35_36ins45;171C>T |
14 | human | 2778 | GNAS | GNAS complex locus | XM_017027819.1 | 81.1% | 80.9% | 0_1ins177;35_36ins45;171C>T |
15 | human | 2778 | GNAS | GNAS complex locus | XM_017027816.1 | 80.3% | 79.1% | (many diffs) |
16 | human | 2778 | GNAS | GNAS complex locus | XM_017027820.1 | 80.3% | 79.1% | (many diffs) |
17 | human | 2778 | GNAS | GNAS complex locus | XR_002958471.1 | 52.3% | (many diffs) | |
18 | human | 2778 | GNAS | GNAS complex locus | NM_080425.3 | 36.8% | 36% | (many diffs) |
19 | human | 2778 | GNAS | GNAS complex locus | XM_017027812.2 | 36.7% | 36% | (many diffs) |
20 | human | 2778 | GNAS | GNAS complex locus | XM_017027813.2 | 35.4% | 34.6% | (many diffs) |
21 | human | 2778 | GNAS | GNAS complex locus | XM_017027814.2 | 35.3% | 34.5% | (many diffs) |
22 | human | 2778 | GNAS | GNAS complex locus | NM_001309842.2 | 21.9% | 21.8% | 258_258delAinsTG;260T>G;261_262ins920 |
23 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_001310083.1 | 95% | 99.7% | (many diffs) |
24 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_201616.2 | 95% | 99.7% | (many diffs) |
25 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498774.1 | 94.8% | 99.4% | (many diffs) |
26 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_001077510.4 | 91.4% | 95.9% | (many diffs) |
27 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498776.1 | 91.2% | 95.6% | (many diffs) |
28 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498777.2 | 83% | 87.4% | (many diffs) |
29 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_017315658.1 | 80.4% | 84.7% | (many diffs) |
30 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498778.2 | 79.5% | 83.6% | (many diffs) |
31 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NR_003258.1 | 64.8% | (many diffs) | |
32 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_010309.4 | 31.9% | 32% | (many diffs) |
33 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_001077507.2 | 30.7% | 30.6% | (many diffs) |
34 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498780.1 | 30.6% | 30.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1248
- ORF length:
- 1182
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatggg ctgcctcggg aacagtaaga ccgaggacca gcgcaacgag gagaaggcgc 121 agcgtgaggc caacaaaaag atcgagaagc agctgcagaa ggacaagcag gtctaccggg 181 ccacgcaccg cctgctgctg ctgggtgctg gagaatctgg taaaagcacc attgtgaagc 241 agatgaggat cctgcatgtt aatgggttta atggagaggg cggcgaagag gacccgcagg 301 ctgcaaggag caacagcgat ggtgagaagg caaccaaagt gcaggacatc aaaaacaacc 361 tgaaagaggc gattgaaacc attgtggccg ccatgagcaa cctggtgccc cccgtggagc 421 tggccaaccc cgagaaccag ttcagagtgg actacattct gagtgtgatg aacgtgcctg 481 actttgactt ccctcccgaa ttctatgagc atgccaaggc tctgtgggag gatgaaggag 541 tgcgtgcctg ctacgaacgc tccaacgagt accagctgat tgactgtgcc cagtacttcc 601 tggacaagat cgacgtgatc aagcaggctg actatgtgcc gagcgatcag gacctgcttc 661 gctgccgtgt cctgacttct ggaatctttg agaccaagtt ccaggtggac aaagtcaact 721 tccacatgtt tgacgtgggt ggccagcgcg atgaacgccg caagtggatc cagtgcttca 781 acgatgtgac tgccatcatc ttcgtggtgg ccagcagcag ctacaacatg gtcatccggg 841 aggacaacca gaccaaccgc ctgcaggagg ctctgaacct cttcaagagc atctggaaca 901 acagatggct gcgcaccatc tctgtgatcc tgttcctcaa caagcaagat ctgctcgctg 961 agaaagtcct tgctgggaaa tcgaagattg aggactactt tccagaattt gctcgctaca 1021 ctactcctga ggatgctact cccgagcccg gagaggaccc acgcgtgacc cgggccaagt 1081 acttcattcg agatgagttt ctgaggatca gcactgccag tggagatggg cgtcactact 1141 gctaccctca tttcacctgc gctgtggaca ctgagaacat ccgccgtgtg ttcaacgact 1201 gccgtgacat cattcagcgc atgcaccttc gtcagtacga gctgctctnn ccaactttct 1261 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac