Construct: ORF ccsbBroadEn_15937
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005373.1_s300c1, BRDN0000395025
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTN4 (57142)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57142 | RTN4 | reticulon 4 | NM_007008.3 | 100% | 100% | |
| 2 | human | 57142 | RTN4 | reticulon 4 | NM_153828.3 | 50.4% | 50.4% | (many diffs) |
| 3 | human | 57142 | RTN4 | reticulon 4 | NM_207520.2 | 49.1% | 47.9% | (many diffs) |
| 4 | human | 57142 | RTN4 | reticulon 4 | NR_135830.2 | 36.4% | (many diffs) | |
| 5 | human | 57142 | RTN4 | reticulon 4 | NR_135829.1 | 34.4% | (many diffs) | |
| 6 | human | 57142 | RTN4 | reticulon 4 | NM_001321859.2 | 19.7% | 19.3% | (many diffs) |
| 7 | human | 57142 | RTN4 | reticulon 4 | NM_001321860.1 | 19.7% | 19.3% | (many diffs) |
| 8 | human | 57142 | RTN4 | reticulon 4 | NM_001321861.1 | 19.7% | 19.3% | (many diffs) |
| 9 | human | 57142 | RTN4 | reticulon 4 | NM_001321862.1 | 19.7% | 19.3% | (many diffs) |
| 10 | human | 57142 | RTN4 | reticulon 4 | NM_001321863.2 | 19.7% | 19.3% | (many diffs) |
| 11 | human | 57142 | RTN4 | reticulon 4 | NM_001321904.2 | 19.7% | 19.3% | (many diffs) |
| 12 | human | 57142 | RTN4 | reticulon 4 | NM_207521.2 | 19.7% | 19.3% | (many diffs) |
| 13 | human | 57142 | RTN4 | reticulon 4 | NM_020532.5 | 16.3% | 16% | (many diffs) |
| 14 | mouse | 68585 | Rtn4 | reticulon 4 | NM_024226.4 | 92.7% | 97.4% | (many diffs) |
| 15 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194052.3 | 49.1% | 51.9% | (many diffs) |
| 16 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194053.3 | 47.8% | 49.3% | (many diffs) |
| 17 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194051.3 | 17.2% | 17.9% | (many diffs) |
| 18 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194054.3 | 15.5% | 16.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatgga cggtcagaag aaaaattgga aggacaaggt tgttgacctc ctgtactgga 121 gagacattaa gaagactgga gtggtgtttg gtgccagcct attcctgctg ctttcattga 181 cagtattcag cattgtgagc gtaacagcct acattgcctt ggccctgctc tctgtgacca 241 tcagctttag gatatacaag ggtgtgatcc aagctatcca gaaatcagat gaaggccacc 301 cattcagggc atatctggaa tctgaagttg ctatatctga ggagttggtt cagaagtaca 361 gtaattctgc tcttggtcat gtgaactgca cgataaagga actcaggcgc ctcttcttag 421 ttgatgattt agttgattct ctgaagtttg cagtgttgat gtgggtattt acctatgttg 481 gtgccttgtt taatggtctg acactactga ttttggctct catttcactc ttcagtgttc 541 ctgttattta tgaacggcat caggcacaga tagatcatta tctaggactt gcaaataaga 601 atgttaaaga tgctatggct aaaatccaag caaaaatccc tggattgaag cgcaaagctg 661 aatgcccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt 721 gcaacgaac